Rat liver carbonic anhydrase I is not sexually dimorphic but is estrogen repressible

1985 ◽  
Vol 5 (9) ◽  
pp. 735-738 ◽  
Author(s):  
S. Jeffery ◽  
N. D. Carter ◽  
C. A. Wilson
Keyword(s):  
Sexual Dimorphism ◽  
Carbonic Anhydrase ◽  
Sex Hormones ◽  
Rat Liver ◽  
Female Rats ◽  
Sexually Dimorphic ◽  
Male And Female ◽  
Carbonic Anhydrase I ◽  
Male And Female Rats ◽  
Low Levels

Carbonic anhydrase (CA) isozymes CAII and CAIII are known to exhibit sexual dimorphism in rat liver, and the levels of these isozymes are affected by sex hormones. In this paper we show that the isozyme CAI is present at low levels in rat liver, with no difference in concentration between male and female rats. Estrogen and diethylstilbestrol reduce CAI levels in both sexes.

scholarly journals Sex Hormones Regulate Hepatic Fetuin Expression in Male and Female Rats

2014 ◽  
Vol 34 (2) ◽  
pp. 554-564 ◽  
Author(s):  
Sang Woo Kim ◽  
Jung-Won Choi ◽  
Dong Seok Lee ◽  
Jong Won Yun
Keyword(s):  
Sex Hormones ◽  
Female Rats ◽  
Male And Female ◽  
Male And Female Rats

scholarly journals Expression profiling of key pathways in rat liver after a one-year feeding trial with transgenic maize MON810

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Torsten Stein ◽  
Guangyao Ran ◽  
Marc Bohmer ◽  
Soroush Sharbati ◽  
Ralf Einspanier
Keyword(s):  
Rat Liver ◽  
Tissue Level ◽  
Female Rats ◽  
Rna Expression ◽  
Consistent Difference ◽  
Male And Female ◽  
Feeding Groups ◽  
Male And Female Rats ◽  
Maize Mon810 ◽  
One Year

AbstractIn a recent one-year feeding study, we observed no adverse effects on tissue level in organs of rats fed with the genetically-modified maize MON810. Here, we assessed RNA expression levels of 86 key genes of the apoptosis-, NF-кB-, DNA-damage response (DDR)-, and unfolded-protein response (UPR) pathways by RT-qPCR in the rat liver. Male and female rats were fed either with 33% MON810 (GMO), isogenic- (ISO), or conventional maize (CONV) and RNAs were quantified from eight rats from each of the six feeding groups. Only Birc2 transcript showed a significant (p ≤ 0.05) consistent difference of ≥1.5-fold between the GMO and ISO groups in both sexes. Unsupervised cluster analysis showed a strong separation of male and female rats, but no clustering of the feeding groups. Individual analysis of the pathways did not show any clustering of the male or female feeding groups either, though transcript levels of UPR pathway-associated genes caused some clustering of the male GMO and CONV feeding group samples. These differences were not seen between the GMO and ISO control or within the female cohort. Our data therefore does not support an adverse effect on rat liver RNA expression through the long-term feeding of MON810 compared to isogenic control maize.

scholarly journals Differential inhibition of indirect immunofluorescence in rat liver sections incubated with antibodies against microsomal cytochromes P-450a, b, and c and epoxide hydrolase.

1988 ◽  
Vol 36 (3) ◽  
pp. 291-295 ◽  
Author(s):  
D E Moody ◽  
L A Taylor ◽  
E A Smuckler
Keyword(s):  
Rat Liver ◽  
Indirect Immunofluorescence ◽  
Epoxide Hydrolase ◽  
Specific Antigen ◽  
Female Rats ◽  
Frozen Sections ◽  
Male And Female ◽  
No Inhibition ◽  
Male And Female Rats ◽  
Unique Method

Rat liver sections were incubated with antibodies (100-1000 micrograms IgG/ml) against microsomal cytochromes P-450a, P-450b, and P-450c, and epoxide hydrolase. Inhibition of indirect immunofluorescence, which progressed with higher concentrations of primary antibody, corresponded with antigen-enriched tissue in frozen liver sections from male and female rats. It was found in liver sections from phenobarbital-treated rats incubated with anti-P-450b and anti-epoxide hydrolase and from 3-methylcholanthrene-treated rats incubated with anti-P-450c. No inhibition was found in sections from untreated rats or rats receiving treatments that did not induce the specific antigen. No inhibition was found in sections incubated with anti-P-450a. Inhibition of immunofluorescence was abolished in frozen sections subjected to dehydration-rehydration protocols known to extract antigens, and was prevented by certain solvents and detergent-wash. Inhibition of immunofluorescence provides a unique method for confirming the antigen-rich regions of the liver lobules specific for microsomal expoxide hydrolase and the cytochrome P-450s.

PRECOCIOUS 'FEMINIZATION' OF RAT LIVER ENZYMES IN THE PRESENCE OF ECTOPIC PITUITARY TISSUE

1978 ◽  
Vol 76 (2) ◽  
pp. 187-191 ◽  
Author(s):  
JAN-ÅKE GUSTAFSSON ◽  
PAUL SKETT
Keyword(s):  
Rat Liver ◽  
Liver Enzymes ◽  
Female Rats ◽  
Steroid Metabolism ◽  
Male And Female ◽  
Adult Rat ◽  
Female Type ◽  
Pituitary Tissue ◽  
Male And Female Rats

The presence of ectopic pituitary tissue (derived from an adult rat) in prepubertal male and female rats caused the immature, masculine-type hepatic steroid metabolism to develop into female-type metabolism. It is concluded that the hypothalamus–pituitary system controls the ontogenesis of sex-dependent steroid metabolism in rat liver.

scholarly journals The effects of aging on carbonic anhydrase concentrations in rat liver and skeletal muscle

1988 ◽  
Vol 250 (1) ◽  
pp. 303-305 ◽  
Author(s):  
S Jeffery ◽  
B J Merry ◽  
A M Holehan ◽  
N D Carter
Keyword(s):  
Sexual Dimorphism ◽  
Carbonic Anhydrase ◽  
Slow Muscle ◽  
Female Rats ◽  
Carbonic Anhydrase Ii ◽  
Slow Fibre ◽  
Fast Fibre ◽  
Male And Female Rats ◽  
Effects Of Aging ◽  
Fast Muscles

The isoenzymes carbonic anhydrase II (CAII) and III (CAIII) have been measured by radioimmunoassay in the livers of male and female rats aged from 21 to 800 days. No sexual dimorphism at 21 days was found, but from 50 to 400 days both isoenzymes show sexual differences. From 600 days onwards, these differences are less apparent. CAIII concentrations in two ‘fast’ fibre muscles and one ‘slow’ fibre muscle have been determined. There is no sexual dimorphism in muscle, but a wide variation between individuals was observed. Fast muscles show maximal CAIII levels at 800 days, whereas in slow muscle the concentration of the isoenzyme is declining at this time.

scholarly journals Blockage of the Neonatal Leptin Surge Affects the Gene Expression of Growth Factors, Glial Proteins, and Neuropeptides Involved in the Control of Metabolism and Reproduction in Peripubertal Male and Female Rats

Endocrinology ◽  
2015 ◽  
Vol 156 (7) ◽  
pp. 2571-2581 ◽  
Author(s):  
Virginia Mela ◽  
Francisca Díaz ◽  
Ana Belen Lopez-Rodriguez ◽  
María Jesús Vázquez ◽  
Arieh Gertler ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Pubertal Development ◽  
Trophic Factors ◽  
Female Rats ◽  
Sexually Dimorphic ◽  
Mrna Levels ◽  
Long Term Effects ◽  
Male And Female ◽  
Male And Female Rats ◽  
Subcutaneous Adipose

Leptin (Lep) is important in the development of neuroendocrine circuits involved in metabolic control. Because both Lep and metabolism influence pubertal development, we hypothesized that early changes in Lep signaling could also modulate hypothalamic (HT) systems involved in reproduction. We previously demonstrated that a single injection of a Lep antagonist (Antag) on postnatal day (PND)9, coincident with the neonatal Lep peak, induced sexually dimorphic modifications in trophic factors and markers of cell turnover and neuronal maturation in the HT on PND13. Here, our aim was to investigate whether the alterations induced by Lep antagonism persist into puberty. Accordingly, male and female rats were treated with a pegylated super Lep Antag from PND5 to PND9 and killed just before the normal appearance of external signs of puberty (PND33 in females and PND43 in males). There was no effect on body weight, but in males food intake increased, subcutaneous adipose tissue decreased and HT neuropeptide Y and Agouti-related peptide mRNA levels were reduced, with no effect in females. In both sexes, the Antag increased HT mRNA levels of the kisspeptin receptor, G protein-coupled recepter 54 (Gpr54). Expression of the Lep receptor, trophic factors, and glial markers were differently affected in the HT of peripubertal males and females. Lep production in adipose tissue was decreased in Antag-treated rats of both sexes, with production of other cytokines being differentially regulated between sexes. In conclusion, in addition to the long-term effects on metabolism, changes in neonatal Lep levels modifies factors involved in reproduction that could possibly affect sexual maturation.

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