High-mannose oligosaccharides from human Tamm-Horsfall glycoprotein

1984 ◽  
Vol 4 (3) ◽  
pp. 269-274 ◽  
Author(s):  
Franca Serafini-Cessi ◽  
Fabio Dall'olio ◽  
Nadia Malagolini
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Gel Filtration ◽  
Chromatographic Mobility ◽  
Present Communication ◽  
Complex Type ◽  
High Mannose ◽  
Layer Chromatography

The present communication reports the occurrence of high-mannose oligosaccharides on Tamm-Horsfall glycoprotein prepared from human pooled urine. The Pronase digest of the glycoprotein was fractionated by gel filtration and a high-mannose glycopeptide species was separated from complex-type glycopeptides. When high-mannose glycopeptides were digested with endo-β-N-acetylglucosaminidase H, followed by reduction with [3H]KBH4, three oligosaccharides were resolved by thin-layer chromatography. On the basis of chromatographic mobility and exoglycosidase digestions the composition Man7-, Man6-, and Man5-GlcNAc was assigned to the three oligosaccharides. Man6GlcNAc is by far the major component.

Alkylresorcinol Homologs in Pisum sativum L. Varieties

1999 ◽  
Vol 54 (1-2) ◽  
pp. 44-48 ◽  
Author(s):  
Robert Zarnowski ◽  
Arkadiusz Kozubek
Keyword(s):  
Pisum Sativum ◽  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Chain Length ◽  
Homologous Series ◽  
Chromatographic Mobility ◽  
Fast Blue ◽  
Pisum Sativum L ◽  
Acetone Extracts ◽  
Layer Chromatography

Acetone extracts from the seeds of Pisum sativum L. sensu lato (Leguminoseae) separated by thin layer chromatography revealed the occurrence of hands with chromatographic mobility and color reaction with Fast Blue B characteristic for 1,3-dihydroxy-5-alkyIbenzenes. These polyketide metabolites have been isolated and identified by Spectroscopic means. The occurrence of homologous series of saturated (approximately 70%) and enoic (mono and diunsaturated) homologs with chain length of C15 to C25 has been revealed with C17 as the main homolog

Screening Method for the Detection of Aflatoxins, Ochratoxin, Patulin, Sterigmatocystin, and Zearalenone in Cereals

1977 ◽  
Vol 60 (6) ◽  
pp. 1369-1371 ◽  
Author(s):  
B G Egon Josefsson ◽  
Tord E Möller
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Ochratoxin A ◽  
Screening Method ◽  
Gel Filtration ◽  
Limits Of Detection ◽  
Layer Chromatography

Abstract A screening method has been developed for the detection of aflatoxins, ochratoxin A, patulin, sterigmatocystin, and zearalenone in cereals. After extraction, the sample is cleaned up by gel filtration. The mycotoxins are separated by thin layer chromatography. The limits of detection are about 5 μg aflatoxins, 10 ochratoxin A, 50 μg patulin, 10 μg sterigmatocystin, and 35 μg zearalenone/kg.

Studies of the Chemical and Biological Properties of the Skeletal Imaging Agent 99mTc-Methylene Diphosphonate

1982 ◽  
Vol 21 (04) ◽  
pp. 150-156 ◽  
Author(s):  
D. Djokić ◽  
N. Paunković ◽  
D. Gorkić ◽  
N. Vanlić-Razumenić
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Clinical Application ◽  
Paper Chromatography ◽  
Gel Filtration ◽  
Biological Properties ◽  
Clinical Applications ◽  
Molar Ratio ◽  
Kidney Imaging ◽  
Layer Chromatography

Studies of the chemical and biological properties as well as of the optimization of a 99mTc-MDP kit for clinical application have been performed. During the preparation of a Sn-MDP complex for labelling with 99mTc, the molar ratio of the reactants [MDP: Sn (II)], the concentration of the Sn-MDP complex and the pH of the 99mTc-MDP solution have been varied and their effects on biodistribution examined. The radiochemical composition of the labelled solutions was checked by paper chromatography, thin-layer chromatography and gel-filtration. The results of clinical applications for bone and early kidney imaging are also reported.

Simple laboratory determination of excess oligosacchariduria.

1981 ◽  
Vol 27 (2) ◽  
pp. 243-245 ◽  
Author(s):  
A C Sewell
Keyword(s):  
Thin Layer ◽  
Thin Layer Chromatography ◽  
Storage Disease ◽  
Gel Filtration ◽  
Glycogen Storage ◽  
Small Sample ◽  
Simple Set ◽  
Layer Chromatography ◽  
Beta Galactosidase

Abstract I describe a simple set of procedures for the screening of patients' urine to detect oligosaccharide-storage diseases. Urines from patients with mucolipidosis I, mannosidosis, fucosidosis, aspartylglycosaminuria, and type VI glycogen-storage disease can be distinguished by thin-layer chromatography. Patients with beta-galactosidase deficiency can be detected by use of a combination of ion-exchange and thin-layer chromatography. Excess sialyloligosaccharide excretion is detected by using gel filtration and a quantitative assay for neuraminic acid. The advantages of the system are detection of virtually all known disorders in which oligosaccharides are over-excreted, production of characteristic patterns, and small sample requirement.

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