The effect of osmotic pressure of aqueous PEG solutions on red blood cells

1985 ◽  
Vol 5 (8) ◽  
pp. 689-696 ◽  
Author(s):  
Andreas Herrmann ◽  
Klaus Arnold ◽  
Lothar Pratsch
Keyword(s):  
Electron Spin Resonance ◽  
Polyethylene Glycol ◽  
Red Blood Cells ◽  
Osmotic Pressure ◽  
Electron Spin ◽  
Blood Cells ◽  
Spin Resonance ◽  
Rotational Correlation Times ◽  
Drastic Increase ◽  
Label Molecule

A drastic increase of the intracellular microviscosity of red blood cells in the presence of polyethylene glycol (PEG) was established by electron spin resonance using the small spin label molecule 2,2,6,6-tetramethylpiperidine-N-oxyl-4-one (TEMPONE). The effective osmotic pressure of PEG solutions stressing the cells was estimated by comparison with those cytoplasmic rotational correlation times of TEMPONE measured in NaCl or sucrose containing media of known osmotic pressure.

scholarly journals Associations between High-Sensitivity C-Reactive Protein and Membrane Fluidity of Red Blood Cells in Hypertensive Elderly Men: An Electron Spin Resonance Study

2012 ◽  
Vol 2012 ◽  
pp. 1-5 ◽  
Author(s):  
Kazushi Tsuda
Keyword(s):  
Electron Spin Resonance ◽  
Membrane Fluidity ◽  
Electron Spin ◽  
Blood Cells ◽  
High Sensitivity ◽  
C Reactive Protein ◽  
Elderly Men ◽  
Reactive Protein ◽  
Spin Resonance ◽  
Hs Crp

Recent evidence indicates that high-sensitivity C-reactive protein (hs-CRP), an acute phase of an inflammatory marker, might be associated with atherosclerosis, hypertension, and other cardiovascular diseases. The present study was performed to assess the possible link between plasma hs-CRP and membrane fluidity (a reciprocal value of membrane microviscosity) in hypertensive elderly men. We measured the membrane fluidity of red blood cells (RBCs) in hypertensive and normotensive elderly men using an electron spin resonance and spin-labeling method. Membrane fluidity of RBCs was decreased in hypertensive elderly men compared with normotensive elderly men. Plasma hs-CRP levels were significantly higher in hypertensive elderly men than in normotensive elderly men. In contrast, plasma nitric-oxide- (NO-) metabolite levels were lower in hypertensive elderly men than in normotensive elderly men. The reduced membrane fluidity of RBCs was associated with increased plasma hs-CRP and decreased plasma NO-metabolite levels. In a multivariate regression analysis, plasma hs-CRP was an independent determinant of membrane fluidity of RBCs after adjustment for general risk factors. The results suggest that CRP might have a close correlation with the rheologic behavior of RBCs and the microcirculation and would contribute, at least in part, to the circulatory dysfunction and vascular complications in hypertensive elderly men.

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