Influence of the intracellular and extracellular cation concentration on monovalent cation efflux of resealed human erythrocyte ghosts

1985 ◽  
Vol 5 (5) ◽  
pp. 425-432 ◽  
Author(s):  
Peter Müller ◽  
Andreas Herrmann ◽  
Ingolf Bernhardt ◽  
Roland Glaser
Keyword(s):  
Ion Transport ◽  
Rate Constants ◽  
Human Erythrocyte ◽  
Permeability Coefficient ◽  
Membrane Surface ◽  
Biological Membranes ◽  
Monovalent Cation ◽  
Erythrocyte Ghosts ◽  
Surface Potentials ◽  
Goldman Equation

Tracer efflux measurements (86Rb+ and2NaNa+) were performed on resealed human erythrocyte ghosts at different intra- and extracellular NaCI concentrations. Using a modified Goldman equation the observed alterations of the rate constants could be explained by taking into account the transmembrane and surface potentials, at constant permeability coefficient. These results emphasize the importance of membrane surface potentials in triggering ion transport across biological membranes.

Role of spectrin in membrane fusion and in shape change of human erythrocyte ghost

1978 ◽  
Vol 36 (2) ◽  
pp. 328-329
Author(s):  
Hideo Hayashi ◽  
Yoshikazu Hirai ◽  
John T. Penniston
Keyword(s):  
Conformational Changes ◽  
Human Erythrocyte ◽  
Shape Change ◽  
Membrane Surface ◽  
Slight Modification ◽  
Active Role ◽  
Main Role ◽  
Bank Blood ◽  
Human Erythrocyte Ghost

Spectrin is a membrane associated protein most of which properties have been tentatively elucidated. A main role of the protein has been assumed to give a supporting structure to inside of the membrane. As reported previously, however, the isolated spectrin molecule underwent self assemble to form such as fibrous, meshwork, dispersed or aggregated arrangements depending upon the buffer suspended and was suggested to play an active role in the membrane conformational changes. In this study, the role of spectrin and actin was examined in terms of the molecular arrangements on the erythrocyte membrane surface with correlation to the functional states of the ghosts.Human erythrocyte ghosts were prepared from either freshly drawn or stocked bank blood by the method of Dodge et al with a slight modification as described before. Anti-spectrin antibody was raised against rabbit by injection of purified spectrin and partially purified.

Lead and calcium transport in human erythrocyte

1999 ◽  
Vol 18 (5) ◽  
pp. 327-332 ◽  
Author(s):  
J V Calderón-Salinas ◽  
M A Quintanar-Escorcia ◽  
M T González-Martínez ◽  
C E Hernández-Luna
Keyword(s):  
Transport System ◽  
Human Erythrocyte ◽  
Calcium Transport ◽  
Human Erythrocytes ◽  
The Other ◽  
Erythrocyte Ghosts ◽  
Passive Transport ◽  
Ca Uptake

In this paper we report the lead (Pb) and calcium (Ca) uptake by erythrocyte ghosts. In both cases the transport was carried out by a passive transport system with two kinetic components (Michaelis-Menten and Hill). Pb and Ca were capable of inhibiting the transport of the other metal in a non-competitive way. Under hyperpolarization, the uptakes of Ca and Pb were enhanced and the Michaelis-Menten component prevailed. Both Ca and Pb uptakes were inhibited by N-ethyl-maleimide to the same extent. These results indicate that Pb and Ca share the same permeability pathway in human erythrocytes and that this transport system is electrogenic.

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