Incorporation of 3-deoxy-3-fluoro-D-glucose into glycogen and trehalose in fat body and flight muscle in Locusta migratoria

1986 ◽  
Vol 6 (3) ◽  
pp. 309-316 ◽  
Author(s):  
Moses Agbanyo ◽  
Norman F. Taylor
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Acid Hydrolysis ◽  
Chromatographic Analysis ◽  
Flight Muscle ◽  
Fat Body ◽  
Locusta Migratoria ◽  
Tissue Extracts ◽  
Hydrolysis Of ◽  
Nuclear Magnetic

Flight muscle and fat body extracts from Locusta migratoria were incubated with D-[U-14C]-glucose or D-[3-3H]-3-deoxy-3-fluoroglucose and the products were analyzed. In the case of the latter compound, radio-chromatographic analysis yielded glycogen and trehalose fractions that were shown by19F nuclear magnetic resonance to contain fluorine. Acid hydrolysis of these fractions liberated tritium labelled 3-deoxy-3-fluoro-D-glucose. In addition to the formation of “fluoroglycogen” and “fluorotrehalose” in these tissue extracts, there was an accumulation of tritium labelled fructose.

scholarly journals Identification of 6-methylmercaptopurine derivative formed during acid hydrolysis of thiopurine nucleotides in erythrocytes, using liquid chromatography–mass spectrometry, infrared spectroscopy, and nuclear magnetic resonance assay

1998 ◽  
Vol 44 (12) ◽  
pp. 2511-2515 ◽  
Author(s):  
Thierry Dervieux ◽  
Roselyne Boulieu
Keyword(s):  
Mass Spectrometry ◽  
Nuclear Magnetic Resonance ◽  
Liquid Chromatography ◽  
Magnetic Resonance ◽  
Acid Hydrolysis ◽  
Dihydrogen Phosphate ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Hydrolysis Of ◽  
Nuclear Magnetic

Abstract 6-Thioguanine and 6-methylmercaptopurine (Me6-MP) nucleotides are the two major thiopurine metabolites of azathioprine found in erythrocytes. During the acid hydrolysis required for the conversion of thiopurine nucleotides into their free bases, Me6-MP was converted into a compound that could be analyzed on a Purospher RP18-e column with dihydrogen phosphate-methanol buffer as eluent. The pH of the acid extract strongly influenced the conversion of Me6-MP into its derivative. The Me6-MP derivative was identified using liquid chromatography–mass spectrometry and infrared and nuclear magnetic resonance spectrometric methods. During the acid hydrolysis of thiopurine nucleotides in erythrocytes, Me6-MP undergoes degradation, leading to 4-amino-5-(methylthio)carbonyl imidazole.

scholarly journals Ellagitannins and Oligomeric Proanthocyanidins of Three Polygonaceous Plants

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 337
Author(s):  
Yun-Qiu Li ◽  
Masako Kitaoka ◽  
Juri Takayoshi ◽  
Ya-Feng Wang ◽  
Yosuke Matsuo ◽  
...  
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Acid Hydrolysis ◽  
Ellagic Acid ◽  
Hydrolyzable Tannin ◽  
Hydrolyzable Tannins ◽  
Oligomeric Proanthocyanidins ◽  
Spectroscopic Examination ◽  
Hydrolysis Of ◽  
Nuclear Magnetic

The aim of this study was to characterize hydrolyzable tannins in Polygonaceous plants, as only a few plants have previously been reported to contain ellagitannins. From Persicaria chinensis, a new hydrolyzable tannin called persicarianin was isolated and characterized to be 3-O-galloyl-4,6-(S)-dehydrohexahydroxydiphenoyl-d-glucose. Interestingly, acid hydrolysis of this compound afforded ellagic acid, despite the absence of a hexahydroxydiphenoyl group. From the rhizome of Polygonum runcinatum var. sinense, a large amount of granatin A, along with minor ellagitannins, helioscpoinin A, davicratinic acids B and C, and a new ellagitannin called polygonanin A, were isolated. Based on 2D nuclear magnetic resonance (NMR) spectroscopic examination, the structure of polygonanin A was determined to be 1,6-(S)-hexahydroxydiphenoyl-2,4-hydroxychebuloyl-β-d-glucopyranose. These are the second and third hydrolyzable tannins isolated from Polygonaceous plants. In addition, oligomeric proanthocyanidins of Persicaria capitatum and P. chinensis were characterized by thiol degradation. These results suggested that some Polygonaceous plants are the source of hydrolyzable tannins not only proanthocyanidins.

FORMATION OF DIASTEREOISOMERIC ORTHOACETATES OF D-MANNOSE: N.M.R. SPECTRAL EVIDENCE

1963 ◽  
Vol 41 (2) ◽  
pp. 399-406 ◽  
Author(s):  
A. S. Perlin
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Magnetic Resonance ◽  
Acid Hydrolysis ◽  
Chemical Shifts ◽  
Isomer Ratio ◽  
Methyl Group ◽  
Major Isomer ◽  
Spectral Evidence ◽  
Ratio Data ◽  
Nuclear Magnetic

Diastereoisomeric 1,2-orthoacetates of D-mannose are formed under conditions of the Königs–Knorr synthesis. This is shown by the isolation of two crystalline isomers of β-D-mannose 1,2-(benzyl orthoacetate), and also by examination of the nuclear magnetic resonance (n.m.r.) spectra of several D-mannose orthoacetates. The diastereoisomers show notable differences in respective chemical shifts for the C-methyl and other protons and these variations, together with isomer-ratio data, suggest that the major isomer of a given pair possesses the configuration in which the C-methyl group is endo- and the OR group is exo-. On acid hydrolysis the orthoacetates yield mainly 2-O-acetyl-D-mannose, the structure of which is confirmed by n.m.r. spectral evidence.

Structural analysis of the three lipopolysaccharides produced by Salmonella madelia (1,6,14,25)

1989 ◽  
Vol 67 (2-3) ◽  
pp. 78-85 ◽  
Author(s):  
José L. Di Fabio ◽  
Jean-Robert Brisson ◽  
Malcolm B. Perry
Keyword(s):  
Nuclear Magnetic Resonance ◽  
Acetic Acid ◽  
Magnetic Resonance ◽  
Nitrous Acid ◽  
Periodate Oxidation ◽  
Ps Ii ◽  
Polysaccharide Fraction ◽  
Ps I ◽  
Hydrolysis Of ◽  
Nuclear Magnetic

Salmonella madelia reported to express the O-antigenic factors 1, 6, 14, and 25, defined in the Kauffmann–White classification system, was found to produce three different homogeneous lipopolysaccharides, which differed in having three structurally distinct O-polysaccharide components. The O-polysaccharide fraction obtained by mild acetic acid hydrolysis of the S. madelia lipopolysaccharide was analyzed by chemical composition, nitrous acid deamination, periodate oxidation, methylation, and 1H and 13C nuclear magnetic resonance methods and was demonstrated to be composed of three polysaccharides, PS(I), PS(II), and PS(III), which had the structures of repeating oligosaccharide units:[Formula: see text]Key words: Salmonella madelia, lipopolysaccharide, structure, analysis, nuclear magnetic resonance.

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