Determination of polyphenolic compounds in leaf protein concentrates of lucerne and their effect on the nutritional value

1983 ◽  
Vol 33 (2-3) ◽  
pp. 169-172 ◽  
Author(s):  
J. C. Rambourg ◽  
B. Monties
Keyword(s):  
Nutritional Value ◽  
Polyphenolic Compounds ◽  
Leaf Protein ◽  
Protein Concentrates

scholarly journals Determination of protein and reactive lysine in leaf-protein concentrates by dye-binding

1979 ◽  
Vol 42 (3) ◽  
pp. 445-454 ◽  
Author(s):  
Ann F. Walker
Keyword(s):  
Convenient Method ◽  
Nutritional Value ◽  
Tungstic Acid ◽  
Leaf Protein ◽  
Processing Conditions ◽  
Acid Orange ◽  
Protein Concentrates ◽  
Dye Binding ◽  
Available Lysine

1. Twenty leaf-protein concentrates (LPC), were produced from different crops and by different processes, the latter being designed to retain maximum nutritional value of the samples.2. The establishment of conditions for the use of CI Acid Orange 12 in a commercial dye-buffer reagent for the determination of protein and reactive (available) lysine in LPC was investigated.3. Values for protein by dye-binding correlated well with those for tungstic-acid-precipitated nitrogen (×6.25).4. Some LPC samples showed a loss of reactive lysine, the greatest loss being associated with the most severe processing conditions.5. For the LPC samples studied, dye-binding provided a convenient method for the concurrent determination of protein and reactive lysine.

Quantitative Determination of Coumestrol in Dried Alfalfa and Alfalfa Leaf Protein Concentrates Containing Chlorophyll

1975 ◽  
Vol 58 (5) ◽  
pp. 983-986
Author(s):  
Benny E Knuckles ◽  
Raymond E Miller ◽  
E M Bickoff
Keyword(s):  
Quantitative Determination ◽  
Analytical Method ◽  
Leaf Protein ◽  
Improved Method ◽  
Alcohol Extract ◽  
Protein Concentrates ◽  
Coefficients Of Variation ◽  
Lower Standard ◽  
Alfalfa Leaf

Abstract An improved analytical method for the determination of coumestrol in dried alfalfa and leaf protein concentrates is described. In this method, chlorophyll is removed from an alcohol extract prior to the paper chromatographic-fluorometric measurement of coumestrol. Ninety-eight per cent of the coumestrol added to alfalfa leaf protein concentrates is recovered by this method. This improved method gives replicate values with lower standard deviations and coefficients of variation than the literature method.

THE POLY-PROTEIN PROCESS: A NEW METHOD FOR OBTAINING LEAF PROTEIN CONCENTRATES

1977 ◽  
Vol 42 (5) ◽  
pp. 1401-1401 ◽  
Author(s):  
G. ANELLI ◽  
R. FIORENTINI ◽  
L. MASSIGNAN ◽  
C. GALOPPINI
Keyword(s):  
New Method ◽  
Leaf Protein ◽  
Protein Concentrates

Hemodynamic control using models of electroacoustic signals of an oxygenated neuron

2021 ◽  
Vol 21 (3) ◽  
pp. 50-54
Author(s):  
B.G. Tsugkiev ◽  
◽  
L.Ch. Gagieva ◽  
T.V. Tsugkieva ◽  
M.M. Chegaev ◽  
...  
Keyword(s):  
Heavy Metals ◽  
Nutritional Value ◽  
Growing Season ◽  
Comparative Assessment ◽  
Biologically Active ◽  
Onobrychis Viciifolia ◽  
Iron Copper ◽  
Calcium Phosphorus ◽  
Active Substances

The paper presents a comparative assessment of the nutritional value depending on the phenological phase of Onobrychis viciifolia Scop., as well as the determination of the regularity of the accumulation of biologically active substances and heavy metals in the herb of the species. The yield of green mass of plants was determined. From each hectare of sowing, 9,7 centners of protein can be obtained; the yield of fiber is 13 centners, BEV is 22,9 centners, fat is 1,6 centners, ash is 4 centners and carotene is 13.0 kg. Depending on the growing season, the chemical composition in the grass of Onobrychis viciifolia changes. The content of calcium, phosphorus increases, and the presence of iron, copper, cobalt, zinc, manganese and molybdenum and amino acids decreases.

scholarly journals High throughput method development and optimised production of leaf protein concentrates with potential to support the agri-industry

2021 ◽  
Author(s):  
Ajay Iyer ◽  
Lisa Guerrier ◽  
Salomé Leveque ◽  
Charles S. Bestwick ◽  
Sylvia H. Duncan ◽  
...  
Keyword(s):  
Amino Acid ◽  
High Throughput ◽  
High Throughput Screening ◽  
Protein Extraction ◽  
Leaf Protein ◽  
Protein Recovery ◽  
Screening Methods ◽  
Extraction Technology ◽  
Protein Concentrates ◽  
Ethanol Precipitation

AbstractInvasive plants offer an interesting and unconventional source of protein and the considerable investment made towards their eradication can potentially be salvaged through their revalorisation. To identify viable sources, effective and high-throughput screening methods are required, as well as efficient procedures to isolate these components. Rigorous assessment of low-cost, high-throughput screening assays for total sugar, phenolics and protein was performed, and ninhydrin, Lever and Fast Blue assays were found to be most suitable owing to high reliability scores and false positive errors less than 1%. These assays were used to characterise invasive Scottish plants such as Gorse (Ulex europeans), Broom (Cystisus scoparius) and Fireweed (Chamaenerion angustifolium). Protein extraction (alkali-, heat- and enzyme assisted) were tested on these plants, and further purification (acid and ethanol precipitation, as well as ultrafiltration) procedures were tested on Gorse, based on protein recovery values. Cellulase treatment and ethanol precipitation gave the highest protein recovery (64.0 ± 0.5%) and purity (96.8 ± 0.1%) with Gorse. The amino acid profile of the purified protein revealed high levels of essential amino acids (34.8 ± 0.0%). Comparison of results with preceding literature revealed a strong association between amino acid profiles and overall protein recovery with the extraction method employed. The final purity of the protein concentrates was closely associated to the protein content of the initial plant mass. Leaf protein extraction technology can effectively raise crop harvest indices, revalorise underutilised plants and waste streams.

DETERMINATION OF POLYPHENOLIC COMPOUNDS OF LYSIMACHIA NUMMULARIA L.

2021 ◽  
Author(s):  
Suciu Felicia ◽  
Roșca Adrian Cosmin ◽  
Lupu Carmen ◽  
Popescu Antoanela ◽  
Badea Victoria
Keyword(s):  
Extraction Process ◽  
Polyphenolic Compounds ◽  
Polyphenol Content ◽  
Total Polyphenol ◽  
Important Place ◽  
Spectrophotometric Methods ◽  
Total Polyphenol Content ◽  
History Of ◽  
Different Parts

"The history of medicinal plants is associated with the evolution of civilization. In all regions of the world, the history of nations shows that these plants have always occupied an important place in medicine, in cosmetic products, and culinary preparations. The paper aims to determine the total polyphenols in different parts of the species Lysimachia nummularia L. In our study, we focused on the extraction of polyphenolic compounds in different solvents. The solvents used in the extraction were: 40% ethanol, concentrated methanol, in water. The total polyphenol content was determined by spectrophotometric methods, a method from the European Pharmacopoeia 10.0, with minor modifications. The total polyphenol content of different extracts varied depending on the extraction process. Different parts of the plant and different solvents were used in the determinations carried out to establish the optimal extraction method for the organs of Lysimachia nummularia L."

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