Altered permeability of the erythrocyte membrane for sodium and potassium ions in spontaneously hypertensive rats

1976 ◽  
Vol 365 (2-3) ◽  
pp. 257-263 ◽  
Author(s):  
Yu. Postnov ◽  
S. Orlov ◽  
P. Gulak ◽  
A. Shevchenko
Keyword(s):  
Erythrocyte Membrane ◽  
Spontaneously Hypertensive Rats ◽  
Potassium Ions ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Sodium And Potassium

Evidence of Altered Permeability of the Erythrocyte Membrane for Sodium and Potassium ions in Spontaneously Hypertensive Rats

1976 ◽  
Vol 51 (s3) ◽  
pp. 169s-172s ◽  
Author(s):  
YU. V. Postnov ◽  
S. N. Orlov ◽  
P. V. Gulak ◽  
A. S. Shevchenko
Keyword(s):  
Erythrocyte Membrane ◽  
Spontaneously Hypertensive Rats ◽  
Potassium Ions ◽  
Sprague Dawley ◽  
Hypertensive Rats ◽  
Sodium Potassium ◽  
Spontaneously Hypertensive ◽  
Sprague Dawley Rats ◽  
Sodium Potassium Pump ◽  
Sodium And Potassium

1. Erythrocyte membrane permeability to sodium and potassium ions was studied in 8–10 weeks spontaneously hypertensive rats (SHR, Kyoto/Wistar strain), normotensive Wistar and Sprague—Dawley rats. 2. The rate of 22Na efflux from the erythrocytes and the rate constant of Na/Na exchange were considerably greater in SHR than in normotensive Wistar and Sprague—Dawley rats. This difference remained the same in rats adrenalectomized 7 days before the experiment. The maximum difference in the constants was found when the sodium—potassium pump was blocked by ouabain. 3. The accumulation of 42K in the erythrocytes of SHR (the sodium—potassium pump being blocked) took place at a considerably slower rate, and the K+ wash-out into a potassium-free medium was faster than that in the normotensive Wistar and Sprague—Dawley rats. 4. These results indicate a higher permeability of the erythrocyte membrane of SHR for Na+ and K+, compared with normotensive Wistar and Sprague—Dawley strains. It is suggested that this may reflect a more widespread cell-membrane defect, which could serve as a general cause for activating the mechanisms maintaining high blood pressure.

Effect of chronic NG-nitro-L-arginine methyl ester (L-NAME) on blood pressure and renal function in conscious uninephrectomized spontaneously hypertensive rats

1998 ◽  
Vol 76 (1) ◽  
pp. 63-67 ◽  
Author(s):  
María Reverte ◽  
Olga Flores ◽  
Belén Gallego ◽  
Antonio Lestón ◽  
José Miguel López-Novoa
Keyword(s):  
Blood Pressure ◽  
Drinking Water ◽  
Renal Function ◽  
Methyl Ester ◽  
Spontaneously Hypertensive Rats ◽  
Low Dose ◽  
Potassium Excretion ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Sodium And Potassium

We have studied during 30 days the effect of a low dose of NG-nitro-L-arginine methyl ester (1 mg ·kg-1 ·day-1 in drinking water) in the presence of D- or L-arginine (1 mg ·kg-1 ·day-1 in drinking water) in comparison with D- or L-arginine alone on blood pressure and renal function in conscious uninephrectomized female spontaneously hypertensive rats. At the end of the study, there was a significant increase in systolic blood pressure in the NG-nitro-L-arginine methyl ester + D-arginine group (307 ± 6 mmHg (1 mmHg = 133.3 Pa), n = 14, p << 0.05) in comparison with NG-nitro-L-arginine methyl ester + L-arginine (281 ± 6 mmHg, n = 14), L-arginine (262 ± 5 mmHg, n = 13), and D-arginine (258 ± 7 mmHg, n = 12) groups. There were no changes in diuresis, proteinuria, or sodium and potassium excretion between differently treated animals during this study. These results suggest that in uninephrectomized female spontaneously hypertensive rats, after 1 month blockade of NO synthesis with a low dose of NG-nitro-L-arginine methyl ester, vasculature is under tonic control by NO and it is not correlated with renal dysfunction.Key words: Key words: NG -nitro-L-arginine methyl ester (L-NAME), kidney, hypertension, spontaneously hypertensive rats, renaldysfunction, uninephrectomy.

Decreased Phosphorylation of Polyphosphoinositides in the Erythrocyte Membrane of Spontaneously Hypertensive Rats

1982 ◽  
Vol 63 (s8) ◽  
pp. 37s-39s ◽  
Author(s):  
P. Marche ◽  
S. Koutouzov ◽  
P. Meyer
Keyword(s):  
Erythrocyte Membrane ◽  
Calcium Binding ◽  
Spontaneously Hypertensive Rats ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Wistar Kyoto Rats ◽  
Inositol Phospholipids ◽  
Wistar Kyoto

1. The distribution and metabolism of inositol phospholipids were studied in the erythrocyte membrane of spontaneously hypertensive and normotensive Wistar-Kyoto rats. The incorporation of 32P into these lipids was considered as an index of their turnover, and a reflection of their metabolism. 2. With [γ-32P]ATP as the source of label only the polyphosphoinositides (diphosphoinositide and triphosphoinositide) incorporated 32P. The levels of labelling were lower in hypertensive than in normotensive rats. Our data indicate that, in the hypertensive rats, the kinases responsible for the labelling of polyphosphoinositides exhibited decreased Vmax. for ATP. 3. These changes may be related to those described for calcium binding and transport in the erythrocyte of spontaneously hypertensive rats.

Evidence of Altered Structure of the Erythrocyte Membrane in Spontaneously Hypertensive Rats

1982 ◽  
Vol 63 (1) ◽  
pp. 43-45 ◽  
Author(s):  
S. N. Orlov ◽  
P. V. Gulak ◽  
I. S. Litvinov ◽  
YU. V. Postnov
Keyword(s):  
Arterial Hypertension ◽  
Erythrocyte Membrane ◽  
Fluorescent Probes ◽  
Membrane Structure ◽  
Spontaneously Hypertensive Rats ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive ◽  
Hydrophobic Areas ◽  
Renal Hypertensive Rats ◽  
Normotensive Control

1. The membrane structure of erythrocytes of rats with different forms of arterial hypertension was studied by means of two hydrophobic fluorescent probes (diphenylhexatriene and pyrene). 2. Microviscosity of hydrophobic areas of erythrocyte membrane of spontaneously hypertensive rats was found to be increased compared with that of membranes from normotensive control rats. 3. No alterations of membrane structure of erythrocytes of deoxycorticosterone-treated rats and renal hypertensive rats were found.

Calcium Accumulation and Calcium Binding by the Cell Membranes of Cardiomyocytes and Smooth Muscle of Aorta in Spontaneously Hypertensive Rats

1980 ◽  
Vol 59 (s6) ◽  
pp. 207s-209s ◽  
Author(s):  
S. N. Orlov ◽  
Yu. V. Postnov
Keyword(s):  
Smooth Muscle ◽  
Erythrocyte Membrane ◽  
Calcium Binding ◽  
Spontaneously Hypertensive Rats ◽  
Plasma Membranes ◽  
Hypertensive Rats ◽  
Binding Ability ◽  
Spontaneously Hypertensive ◽  
Aortic Smooth Muscle ◽  
Lower Ability

1. The ability of isolated membrane fractions of cardiomyocytes, aortic smooth muscle and erythrocytes from spontaneously hypertensive rats to bind calcium was investigated isotopically. 2. Plasma membranes of aortic smooth muscle and erythrocytes of spontaneously hypertensive rats possess a lower calcium-binding ability than preparations from normotensive controls. 3. Sarcoplasmic reticulum from the myocardium of spontaneously hypertensive rats had a lower ability to accumulate calcium. 4. Changes in the calcium-binding ability of the erythrocyte membrane of spontaneously hypertensive rats was related to the diminution of the amount of exchangeable calcium on the inner part of the membrane. The latter may be the cause of the increase of erythrocyte membrane permeability for sodium in spontaneously hypertensive rats and in patients with essential hypertension.

Microcalorimetry and Electrophoresis of the Erythrocyte Membrane of Spontaneously Hypertensive Rats

1984 ◽  
Vol 2 (1) ◽  
pp. 81-84 ◽  
Author(s):  
Pavel V. Gulak ◽  
Sergei N. Orlov ◽  
Nikolai I. Pokudin ◽  
Yuvenali V. Postnov ◽  
Ivan S. Litvinov ◽  
...  
Keyword(s):  
Erythrocyte Membrane ◽  
Spontaneously Hypertensive Rats ◽  
Hypertensive Rats ◽  
Spontaneously Hypertensive
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