Residence time distributions of solutes in the perfused rat liver using a dispersion model of hepatic elimination: 2. Effect of pharmacological agents, retrograde perfusions, and enzyme inhibition on evans blue, sucrose, water, and taurocholate

1990 ◽  
Vol 18 (3) ◽  
pp. 235-258 ◽  
Author(s):  
Michael S. Roberts ◽  
Sharon Fraser ◽  
Andrew Wagner ◽  
Lyndsay McLeod
Keyword(s):  
Enzyme Inhibition ◽  
Rat Liver ◽  
Residence Time ◽  
Evans Blue ◽  
Dispersion Model ◽  
Perfused Rat Liver ◽  
Pharmacological Agents ◽  
Hepatic Elimination ◽  
Residence Time Distributions

Enzymatic basis for the non-linearity of hepatic elimination of propranolol in the isolated perfused rat liver

1992 ◽  
Vol 44 (12) ◽  
pp. 2281-2288 ◽  
Author(s):  
Ryozo Ishida ◽  
Kazuyoshi Suzuki ◽  
Yasuhiro Masubuchi ◽  
Shizuo Narimatsu ◽  
Shoichi Fujita ◽  
...  
Keyword(s):  
Rat Liver ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Hepatic Elimination

scholarly journals Binding and uptake of [3H]adrenaline by perfused rat liver

1984 ◽  
Vol 218 (3) ◽  
pp. 765-773 ◽  
Author(s):  
P H Reinhart ◽  
W M Taylor ◽  
F L Bygrave
Keyword(s):  
Rat Liver ◽  
Significant Proportion ◽  
Induced Responses ◽  
Perfused Rat Liver ◽  
Adrenergic Agonist ◽  
Related Factors ◽  
Pharmacological Agents ◽  
Total Inhibition ◽  
Alpha Adrenergic Agonist

The binding and uptake of [3H]adrenaline by the intact perfused rat liver was investigated. We showed that the administration of [3H]adrenaline to liver resulted in the rapid uptake of the radioligand, and that such uptake was independent of any Ca2+ redistributions induced by the hormone. At low adrenaline concentrations (less than 50 nM) uptake was inhibited by prazosin, whereas at higher hormone concentrations a significant proportion of total [3H]adrenaline uptake could not be inhibited by this antagonist. [3H]Adrenaline uptake could be directly correlated with adrenaline-induced responses such as an increased rate of respiration and glycogenolysis. The partial inhibition (approx. 25%) of [3H]adrenaline uptake by antagonists was sufficient for the total inhibition of hormone-induced responses. The effect of various pharmacological agents on [3H]adrenaline uptake was investigated, and the contribution of tissue-related factors to alpha-adrenergic agonist-antagonist interactions in vivo is discussed.

Liver Blood Flow as a Major Determinant of the Clearance of Recombinant Human Tissue-Type Plasminogen Activator

1992 ◽  
Vol 67 (01) ◽  
pp. 083-087 ◽  
Author(s):  
A de Boer ◽  
C Kluft ◽  
J M Kroon ◽  
F J Kasper ◽  
H C Schoemaker ◽  
...  
Keyword(s):  
Blood Flow ◽  
Rat Liver ◽  
Healthy Subjects ◽  
Liver Blood Flow ◽  
Major Determinant ◽  
Tissue Type ◽  
Perfused Rat Liver ◽  
Liver Model ◽  
Type Plasminogen Activator ◽  
Proportional Change

SummaryThe influence of changes in liver blood flow on the clearance of rt-PA was studied both in healthy subjects and in a perfused rat liver model. Liver blood flow in healthy subjects was documented indirectly by the clearance of indocyanine green (ICG). Exercise reduced liver blood flow on average by 57% with a 95% confidence interval (95% Cl) ranging from 51% to 62% (n = 5) and increased plasma levels of rt-PA activity (after an i. v. infusion of 18 mg of rt-PA over 120 min) by 119% (95% Cl, 58% - 203%) and rt-PA antigen by 91% (95% Cl, 30% - 140%). In the perfused rat liver model it was shown that halving or doubling of the physiological flow rate of a perfusate, containing rt-PA caused a proportional change in the clearance of rt-PA, while the extraction of rt-PA by the liver remained similar. In conclusion, liver blood flow is a major determinant of the clearance of rt-PA. This may have important implications for dosage of rt-PA in patients with myocardial infarction.

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