Residence time distributions of solutes in the perfused rat liver using a dispersion model of hepatic elimination: 1. Effect of changes in perfusate flow and albumin concentration on sucrose and taurocholate

1990 ◽  
Vol 18 (3) ◽  
pp. 209-234 ◽  
Author(s):  
Michael S. Roberts ◽  
Sharon Fraser ◽  
Andrew Wagner ◽  
Lyndsay McLeod
Keyword(s):  
Rat Liver ◽  
Residence Time ◽  
Dispersion Model ◽  
Albumin Concentration ◽  
Perfused Rat Liver ◽  
Hepatic Elimination ◽  
Perfusate Flow ◽  
Residence Time Distributions

scholarly journals Plasma protein catabolism by the perfused rat liver. The effect of alteration of albumin concentration and dietary protein depletion

1970 ◽  
Vol 118 (3) ◽  
pp. 401-404 ◽  
Author(s):  
R. Hoffenberg ◽  
A. H. Gordon ◽  
E. G. Black ◽  
L. N. Louis
Keyword(s):  
Rat Liver ◽  
Dietary Protein ◽  
Plasma Proteins ◽  
Normal Values ◽  
Isolated Perfused Rat Liver ◽  
Albumin Concentration ◽  
Perfused Rat Liver ◽  
Plasma Albumin ◽  
Degradation Rates ◽  
Parenchymal Cells

1. The isolated perfused rat liver was used to study degradation rates of plasma albumin, transferrin and fibrinogen. 2. Constant fractional rates were observed for all three proteins even when the albumin concentration was drastically increased by the addition of large amounts to the perfusate pool. 3. Livers taken from rats deprived of dietary protein for 14–18 days showed greatly diminished fractional catabolic rates for albumin when perfused with blood from similarly deprived animals. 4. These rates could be restored to near-normal values by adding albumin or by perfusing with blood from normally fed rats. 5. These findings are consistent with the theory of pinocytosis as a step in the degradation of plasma proteins by hepatic parenchymal cells.

Enzymatic basis for the non-linearity of hepatic elimination of propranolol in the isolated perfused rat liver

1992 ◽  
Vol 44 (12) ◽  
pp. 2281-2288 ◽  
Author(s):  
Ryozo Ishida ◽  
Kazuyoshi Suzuki ◽  
Yasuhiro Masubuchi ◽  
Shizuo Narimatsu ◽  
Shoichi Fujita ◽  
...  
Keyword(s):  
Rat Liver ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Hepatic Elimination

Effects of hematocrit on oxygenation of the isolated perfused rat liver

1983 ◽  
Vol 245 (6) ◽  
pp. G769-G774 ◽  
Author(s):  
G. L. Riedel ◽  
J. L. Scholle ◽  
A. P. Shepherd ◽  
W. F. Ward
Keyword(s):  
Blood Flow ◽  
Oxygen Uptake ◽  
Portal Vein ◽  
Rat Liver ◽  
Male Rats ◽  
Isolated Perfused Rat Liver ◽  
Perfused Rat Liver ◽  
Flow Rates ◽  
Perfusate Flow ◽  
Hepatic Portal

The isolated perfused rat liver is used ubiquitously for metabolic and endocrine studies of hepatic function, yet few data are available regarding the inadequacy of the oxygenation of such preparations. Moreover, the isolated rat liver is usually deprived of its arterial supply and perfused via the hepatic portal vein with low-hematocrit or cell-free solutions. To investigate the efficacy of the oxygen supply, we determined the effect of hematocrit on the relation between oxygen consumption and perfusate flow. We then attempted to define a hematocrit at which hepatic oxygenation was maximal. Livers of male rats anesthesized with pentobarbital sodium were perfused via the portal vein with fresh canine red blood cells suspended in Krebs-Ringer-bicarbonate buffer. Perfusions were carried out at various flow rates, and the relation between perfusate flow and oxygen uptake was determined. At flow rates above 100 ml X min-1 X 100 g liver-1, oxygen uptake was independent of flow but below that value was flow limited, regardless of whether the hematocrit was 10, 20, or 40%. To determine the optimal hematocrit for hepatic oxygen uptake, hepatic portal venous and hepatic venous pressures were held at 10 and 0 mmHg, respectively. The hematocrit was lowered in steps from 80 to 10%. Blood flow increased exponentially as hematocrit fell while oxygen uptake increased to a maximum at approximately 20%. It is concluded that an hematocrit of approximately 20% provides the optimal combination of blood flow and oxygen-carrying capacity while maintaining physiological perfusion pressures, e.g., 10 mmHg.

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