Sodium and calcium channels in the somatic membrane of artificially differentiated neuroblastoma cells

1986 ◽  
Vol 18 (2) ◽  
pp. 154-159 ◽  
Author(s):  
N. S. Veselovskii ◽  
A. F. Fomina
Keyword(s):  
Calcium Channels ◽  
Neuroblastoma Cells ◽  
Somatic Membrane

scholarly journals Modulation of calcium signaling depends on the oligosaccharide of GM1 in Neuro2a mouse neuroblastoma cells

2020 ◽  
Vol 37 (6) ◽  
pp. 713-727
Author(s):  
Giulia Lunghi ◽  
Maria Fazzari ◽  
Erika Di Biase ◽  
Laura Mauri ◽  
Sandro Sonnino ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Calcium Channels ◽  
Calcium Signaling ◽  
Intracellular Calcium ◽  
Calcium Imaging ◽  
Neuroblastoma Cells ◽  
Ganglioside Gm1 ◽  
Mouse Neuroblastoma ◽  
Neuro2a Cells ◽  
Neuronal Functions

AbstractRecently, we demonstrated that the oligosaccharide portion of ganglioside GM1 is responsible, via direct interaction and activation of the TrkA pathway, for the ability of GM1 to promote neuritogenesis and to confer neuroprotection in Neuro2a mouse neuroblastoma cells. Recalling the knowledge that ganglioside GM1 modulates calcium channels activity, thus regulating the cytosolic calcium concentration necessary for neuronal functions, we investigated if the GM1-oligosaccharide would be able to overlap the GM1 properties in the regulation of calcium signaling, excluding a specific role played by the ceramide moiety inserted into the external layer of plasma membrane. We observed, by calcium imaging, that GM1-oligosaccharide administration to undifferentiated Neuro2a cells resulted in an increased calcium influx, which turned out to be mediated by the activation of TrkA receptor. The biochemical analysis demonstrated that PLCγ and PKC activation follows the TrkA stimulation by GM1-oligosaccharide, leading to the opening of calcium channels both on the plasma membrane and on intracellular storages, as confirmed by calcium imaging experiments performed with IP3 receptor inhibitor. Subsequently, we found that neurite elongation in Neuro2a cells was blocked by subtoxic administration of extracellular and intracellular calcium chelators, suggesting that the increase of intracellular calcium is responsible of GM1-oligosaccharide mediated differentiation. These results suggest that GM1-oligosaccharide is responsible for the regulation of calcium signaling and homeostasis at the base of the neuronal functions mediated by plasma membrane GM1.

L-type calcium channels may regulate neurite initiation in cultured chick embryo brain neurons and N1E-115 neuroblastoma cells

1990 ◽  
Vol 55 (1) ◽  
pp. 109-120 ◽  
Author(s):  
G. Audesirk ◽  
T. Audesirk ◽  
C. Ferguson ◽  
M. Lomme ◽  
D. Shugarts ◽  
...  
Keyword(s):  
Chick Embryo ◽  
Calcium Channels ◽  
Neuroblastoma Cells ◽  
Brain Neurons ◽  
Neurite Initiation ◽  
Embryo Brain
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