scholarly journals Responsiveness of voltage-gated calcium channels in SH-SY5Y human neuroblastoma cells on quasi-three-dimensional micropatterns formed with poly (l-lactic acid)

2013 ◽  
pp. 93 ◽  
Author(s):  
Ze-Zhi Wu ◽  
Wang ◽  
Zhang ◽  
An ◽  
Zhong ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Calcium Channels ◽  
Neuroblastoma Cells ◽  
Three Dimensional ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma ◽  
Voltage Gated ◽  
Voltage Gated Calcium Channels

scholarly journals Exposure of the SH-SY5Y Human Neuroblastoma Cells to 50-Hz Magnetic Field: Comparison Between Two-Dimensional (2D) and Three-Dimensional (3D) In Vitro Cultures

2020 ◽  
Author(s):  
Claudia Consales ◽  
Alessio Butera ◽  
Caterina Merla ◽  
Emanuela Pasquali ◽  
Vanni Lopresto ◽  
...  
Keyword(s):  
Magnetic Field ◽  
Neuroblastoma Cells ◽  
Three Dimensional ◽  
3D Culture ◽  
Cell Cycle Distribution ◽  
Two Dimensional ◽  
3D Scaffold ◽  
Human Neuroblastoma Cells ◽  
Human Neuroblastoma ◽  
3D Cultures

AbstractWe here characterize the response to the extremely low-frequency (ELF) magnetic field (MF, 50 Hz, 1 mT) of SH-SY5Y human neuroblastoma cells, cultured in a three-dimensional (3D) Alvetex® scaffold compared to conventional two-dimensional (2D) monolayers. We proved that the growing phenotype of proliferating SH-SY5Y cells is not affected by the culturing conditions, as morphology, cell cycle distribution, proliferation/differentiation gene expression of 3D-cultures overlap what reported in 2D plates. In response to 72-h exposure to 50-Hz MF, we demonstrated that no proliferation change and apoptosis activation occur in both 2D and 3D cultures. Consistently, no modulation of Ki67, MYCN, CCDN1, and Nestin, of invasiveness and neo-angiogenesis-controlling genes (HIF-1α, VEGF, and PDGF) and of microRNA epigenetic signature (miR-21-5p, miR-222-3p and miR-133b) is driven by ELF exposure. Conversely, intracellular glutathione content and SOD1 expression are exclusively impaired in 3D-culture cells in response to the MF, whereas no change of such redox modulators is observed in SH-SY5Y cells if grown on 2D monolayers. Moreover, ELF-MF synergizes with the differentiating agents to stimulate neuroblastoma differentiation into a dopaminergic (DA) phenotype in the 3D-scaffold culture only, as growth arrest and induction of p21, TH, DAT, and GAP43 are reported in ELF-exposed SH-SY5Y cells exclusively if grown on 3D scaffolds. As overall, our findings prove that 3D culture is a more reliable experimental model for studying SH-SY5Y response to ELF-MF if compared to 2D conventional monolayer, and put the bases for promoting 3D systems in future studies addressing the interaction between electromagnetic fields and biological systems.

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