A histochemical method for the demonstration of unsaturated lipids

1991 ◽  
Vol 23 (2) ◽  
pp. 91-99 ◽  
Author(s):  
O. Fujimori ◽  
H. Ueda ◽  
K. Yamada
Keyword(s):  
Histochemical Method ◽  
Unsaturated Lipids

scholarly journals Histochemical demonstration of unsaturated hydrophilic lipids with palladium chloride.

1977 ◽  
Vol 25 (3) ◽  
pp. 200-205 ◽  
Author(s):  
J A Kiernan
Keyword(s):  
Solvent Extraction ◽  
Standard Technique ◽  
Histochemical Demonstration ◽  
New Method ◽  
Histochemical Method ◽  
Palladium Chloride ◽  
Unsaturated Lipids ◽  
Metallic Palladium

Compounds in which olefinic linkages are accessible to aqueous reagents reduce the chloropalladite ion [PdCl4]2-, to metallic palladium. This reaction is used in a histochemical method whereby hydrophilic unsaturated lipids are stained dark brown or black. The specificity of the new method has been confirmed by means of solvent-extraction and chemical blocking procedures and by comparison with other histochemical techniques. Yellow staining of collagen, keratin and cytoplasm is probably due to attachment of the chloropalladite anion to proteins. The yellow background can be largely decolorized by treating the sections with aqueous pyridine, which forms colorless complexes with divalent palladium. A standard technique for staining with palladium is presented and the method is discussed in relation to other histochemical procedures that demonstrate unsaturated lipids.

Visible-Light Activated [2+2] Photocycloaddition Reaction Enabled Identification of Carbon-Carbon Double Bonds Position Isomerism in Structural Lipidomics

2020 ◽  
Author(s):  
Guifang Feng ◽  
Yanhong Hao ◽  
Liang Wu ◽  
Suming Chen
Keyword(s):  
Visible Light ◽  
Uv Light ◽  
Practical Importance ◽  
Side Reactions ◽  
Potential Health ◽  
Fundamental Interest ◽  
Unsaturated Lipids ◽  
Health Damage ◽  
Global Identification ◽  
Polyunsaturated Lipids

The photocycloaddition of olefins with carbonyls is of fundamental interest and practical importance in C=C bond location in unsaturated lipids. However, the traditional UV light activated [2+2] photocycloaddition reaction suffers side reactions and potential health damage. Here, we reported the first example of visible-light activated [2+2] photocycloaddition of anthraquinone with unsaturated lipids. This reaction showed great capability for locating the C=C bonds in various kinds of monounsaturated and polyunsaturated lipids by combining with tandem mass spectrometry (MS), such as fatty acids, phospholipids and glycerides. Based on this developed reaction, a workflow with liquid chromatography tandem MS method was developed for the global identification of unsaturated lipids in human serum, and 86 of monounsaturated and complicated polyunsaturated lipids were identified with definitive positions of C=C bonds. This approach provides new insights both on the photocycloaddition reactions and the structural lipidomics.

Гистохимический анализ 3β-гидроксистероиддегидрогеназы в гипоталамусе крысы

2020 ◽  
pp. 38-41
Keyword(s):  
Positive Reaction ◽  
Paraventricular Nucleus ◽  
Gray Matter ◽  
Hydroxysteroid Dehydrogenase ◽  
Small Cell ◽  
Histochemical Method ◽  
Peripheral Part ◽  
Arcuate Nuclei ◽  
Different Parts

3β-hydroxysteroid dehydrogenase (GSDG) in different parts of the hypothalamus was studied by histochemical method. It was shown that GSDG-positive reaction was present in neurons of the periventricular gray matter, ventromedial, ventrolateral, and arcuate nuclei of the hypothalamus. In the paraventricular nucleus, GSDG-positive neurons were «small-cell» neurons located primarily in the peripheral part of this nucleus.

scholarly journals Recent Developments in Seafood Packaging Technologies

Foods ◽  
2021 ◽  
Vol 10 (5) ◽  
pp. 940
Author(s):  
Michael G. Kontominas ◽  
Anastasia V. Badeka ◽  
Ioanna S. Kosma ◽  
Cosmas I. Nathanailides
Keyword(s):  
Shelf Life ◽  
Food Packaging ◽  
Modified Atmosphere Packaging ◽  
High Water ◽  
Edible Films ◽  
Nitrogenous Compounds ◽  
Quality Properties ◽  
Unsaturated Lipids ◽  
Active Food Packaging ◽  
Seafood Products

Seafood products are highly perishable, owing to their high water activity, close to neutral pH, and high content of unsaturated lipids and non-protein nitrogenous compounds. Thus, such products require immediate processing and/or packaging to retain their safety and quality. At the same time, consumers prefer fresh, minimally processed seafood products that maintain their initial quality properties. The present article aims to review the literature over the past decade on: (i) innovative, individual packaging technologies applied to extend the shelf life of fish and fishery products, (ii) the most common combinations of the above technologies applied as multiple hurdles to maximize the shelf life of seafood products, and (iii) the respective food packaging legislation. Packaging technologies covered include: Modified atmosphere packaging; vacuum packaging; vacuum skin packaging; active food packaging, including oxygen scavengers; carbon dioxide emitters; moisture regulators; antioxidant and antimicrobial packaging; intelligent packaging, including freshness indicators; time–temperature indicators and leakage indicators; retort pouch processing and edible films; coatings/biodegradable packaging, used individually or in combination for maximum preservation potential.

scholarly journals The pH-Induced Specific Area Changes of Unsaturated Lipids Deposited onto a Bubble Interface

Langmuir ◽  
2021 ◽  
Vol 37 (8) ◽  
pp. 2586-2595
Author(s):  
Nicolas Anton ◽  
Philippe Pierrat ◽  
Germain A. Brou ◽  
Gildas K. Gbassi ◽  
Ziad Omran ◽  
...  
Keyword(s):  
Specific Area ◽  
Unsaturated Lipids

Histochemical Method for Detecting Nitric Oxide Synthase Activity in Cell Cultures

1997 ◽  
Vol 72 (1) ◽  
pp. 29-32 ◽  
Author(s):  
Wei Min Hon ◽  
Vikram Jit Singh Chhatwal ◽  
Hoon Eng Khoo ◽  
Shabbir Mohammed Moochhala
Keyword(s):  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Cell Cultures ◽  
Histochemical Method ◽  
Oxide Synthase ◽  
Nitric Oxide Synthase Activity

Interaction of human serum albumin with liposomes of saturated and unsaturated lipids with different phase transition temperatures: a spectroscopic investigation by membrane probe PRODAN

RSC Advances ◽  
2014 ◽  
Vol 4 (28) ◽  
pp. 14335-14347 ◽  
Author(s):  
Raina Thakur ◽  
Anupam Das ◽  
Anjan Chakraborty
Keyword(s):  
Phase Transition ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Human Serum ◽  
Spectroscopic Techniques ◽  
Time Resolved Fluorescence ◽  
Time Resolved ◽  
Unsaturated Lipids ◽  
Membrane Probe ◽  
Phase Transition Temperatures

The interaction of human serum albumin (HSA) with liposomes made of saturated and unsaturated phosphocholines has been studied using circular dichroism (CD), steady state and time resolved fluorescence spectroscopic techniques.

Sign in / Sign up

Export Citation Format

Share Document