Histochemical method for simultaneous fiber typing and demonstration of capillaries in skeletal muscle

1990 ◽  
Vol 93 (4) ◽  
pp. 385-387 ◽  
Author(s):  
L. Palj�rvi ◽  
A. Naukkarinen
Keyword(s):  
Skeletal Muscle ◽  
Histochemical Method

scholarly journals Calibrated Histochemistry of Myoglobin Concentration in Cardiomyocytes

1998 ◽  
Vol 46 (9) ◽  
pp. 1077-1084 ◽  
Author(s):  
Marleen B.E. ◽  
Lee-de Groot ◽  
Ariane L. des Tombe ◽  
Willem J. van der Laarse
Keyword(s):  
Skeletal Muscle ◽  
Muscle Fibers ◽  
Staining Intensity ◽  
Interstitial Space ◽  
Histochemical Method ◽  
Calibration Line ◽  
Glutaraldehyde Fixation ◽  
Tissue Samples ◽  
Rat Cardiomyocytes ◽  
Skeletal Muscle Fibers

This article describes the calibration of a histochemical method to determine the myoglobin concentration in individual cardiomyocytes. Calibration is based on paired microdensitometric determinations in sections stained for myoglobin and on biochemical myoglobin determinations in tissue samples from different hearts. In addition, the staining intensity of sections from gelatin blocks containing known amounts of myoglobin is determined. To construct a calibration line, sections stained for myoglobin must be corrected for the degree of shrinkage caused by glutaraldehyde fixation and biochemical myoglobin determinations must be corrected for interstitial space. As an example, the method is used to determine the myoglobin concentration in individual skeletal muscle fibers and in control and hypertrophied rat cardiomyocytes. The amount of myoglobin per cardiomyocyte nucleus is increased two- to threefold in hypertrophied cardiomyocytes, whereas changes in myoglobin concentration depend on the model of hypertrophy used.

scholarly journals An Optimized Histochemical Method to Assess Skeletal Muscle Glycogen and Lipid Stores Reveals Two Metabolically Distinct Populations of Type I Muscle Fibers

PLoS ONE ◽  
2013 ◽  
Vol 8 (10) ◽  
pp. e77774 ◽  
Author(s):  
Clara Prats ◽  
Alba Gomez-Cabello ◽  
Pernille Nordby ◽  
Jesper L. Andersen ◽  
Jørn W. Helge ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Muscle Glycogen ◽  
Muscle Fibers ◽  
Histochemical Method ◽  
Type I ◽  
Skeletal Muscle Glycogen ◽  
Lipid Stores

A Histochemical Method for the Simultaneous Demonstration of Capillaries and Fiber Type in Skeletal Muscle

1987 ◽  
Vol 62 (2) ◽  
pp. 85-92 ◽  
Author(s):  
J. David Rosenblatt ◽  
William M. Kuzon ◽  
Michael J. Plyley ◽  
Bruce R. Pynn ◽  
Nancy H. McKee
Keyword(s):  
Skeletal Muscle ◽  
Fiber Type ◽  
Histochemical Method

ELECTROPHORETIC CHARACTERIZATION OF HUMAN DEHYDROGENASES

1965 ◽  
Vol 43 (10) ◽  
pp. 1653-1659 ◽  
Author(s):  
A. M. Katz ◽  
W. Kalow
Keyword(s):  
Skeletal Muscle ◽  
Nicotinamide Adenine Dinucleotide ◽  
Human Skeletal Muscle ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Nicotinamide Adenine ◽  
Histochemical Method ◽  
Reducing Capacity

Several isoenzymes of lactate, malate, and isocitrate dehydrogenases have been demonstrated in human skeletal muscle, heart, and liver. Single zones of activity were shown for glucose-6-phosphate and glutamate dehydrogenases. A zone possessing a marked reducing capacity towards nicotinamide adenine dinucleotide and nicotinamide adenine dinucleotide phosphate was visualized in the absence of any substrate, using the tetrazolium-coupled histochemical method. This zone represents the so-called "nothing dehydrogenase".

Endotoxin Alteration of the Mucopolysaccharide Blood Vessel Coating in Rats

1974 ◽  
Vol 32 ◽  
pp. 148-149
Author(s):  
D. E. Philpott ◽  
A. Takahashi
Keyword(s):  
Skeletal Muscle ◽  
Endothelial Cells ◽  
Salivary Gland ◽  
Carotid Artery ◽  
Basement Membrane ◽  
Coronary Vessels ◽  
Ruthenium Red ◽  
E Coli ◽  
Old Rats ◽  
The Brain

Two month, eight month and two year old rats were treated with 10 or 20 mg/kg of E. Coli endotoxin I. P. The eight month old rats proved most resistant to the endotoxin. During fixation the aorta, carotid artery, basil arartery of the brain, coronary vessels of the heart, inner surfaces of the heart chambers, heart and skeletal muscle, lung, liver, kidney, spleen, brain, retina, trachae, intestine, salivary gland, adrenal gland and gingiva were treated with ruthenium red or alcian blue to preserve the mucopolysaccharide (MPS) coating. Five, 8 and 24 hrs of endotoxin treatment produced increasingly marked capillary damage, disappearance of the MPS coating, edema, destruction of endothelial cells and damage to the basement membrane in the liver, kidney and lung.

Ultra-Rapid Freezing of Isolated Skeletal Muscle Fibers

1977 ◽  
Vol 35 ◽  
pp. 576-577
Author(s):  
Joachim R. Sommer ◽  
Nancy R. Wallace
Keyword(s):  
Skeletal Muscle ◽  
Sarcoplasmic Reticulum ◽  
Granular Material ◽  
Nuclear Envelope ◽  
Intracellular Calcium ◽  
Ruthenium Red ◽  
Threshold Concentration ◽  
Rapid Freezing ◽  
Frog Skeletal Muscle ◽  
Electrical Isolation

After Howell (1) had shown that ruthenium red treatment of fixed frog skeletal muscle caused collapse of the intermediate cisternae of the sarcoplasmic reticulum (SR), forming a pentalaminate structure by obi iterating the SR lumen, we demonstrated that the phenomenon involves the entire SR including the nuclear envelope and that it also occurs after treatment with other cations, including calcium (2,3,4).From these observations we have formulated a hypothesis which states that intracellular calcium taken up by the SR at the end of contraction causes the M rete to collapse at a certain threshold concentration as the first step in a subsequent centrifugal zippering of the free SR toward the junctional SR (JSR). This would cause a) bulk transport of SR contents, such as calcium and granular material (4) into the JSR and, b) electrical isolation of the free SR from the JSR.

Electron Probe Analysis of Muscle

1982 ◽  
Vol 40 ◽  
pp. 398-401
Author(s):  
A. V. Somlyo ◽  
H. Shuman ◽  
A. P. Somlyo
Keyword(s):  
Skeletal Muscle ◽  
Smooth Muscle ◽  
Sarcoplasmic Reticulum ◽  
Resting State ◽  
Binding Sites ◽  
Electron Probe ◽  
Frog Skeletal Muscle ◽  
Electron Probe Analysis ◽  
Probe Analysis

Electron probe analysis of frozen dried cryosections of frog skeletal muscle, rabbit vascular smooth muscle and of isolated, hyperpermeab1 e rabbit cardiac myocytes has been used to determine the composition of the cytoplasm and organelles in the resting state as well as during contraction. The concentration of elements within the organelles reflects the permeabilities of the organelle membranes to the cytoplasmic ions as well as binding sites. The measurements of [Ca] in the sarcoplasmic reticulum (SR) and mitochondria at rest and during contraction, have direct bearing on their role as release and/or storage sites for Ca in situ.

How to sample the entire length of a single muscle fiber quick-frozen after electrical point stimulation for high resolution EM

1992 ◽  
Vol 50 (1) ◽  
pp. 776-777
Author(s):  
Joachim R. Sommer ◽  
Teresa High ◽  
Betty Scherer ◽  
Isaiah Taylor ◽  
Rashid Nassar
Keyword(s):  
Skeletal Muscle ◽  
High Resolution ◽  
Action Potential ◽  
Muscle Fiber ◽  
Freeze Fracture ◽  
Freeze Substitution ◽  
Electrical Field Stimulation ◽  
Skeletal Muscle Fiber ◽  
Freeze Dried ◽  
Quick Freezing

We have developed a model that allows the quick-freezing at known time intervals following electrical field stimulation of a single, intact frog skeletal muscle fiber isolated by sharp dissection. The preparation is used for studying high resolution morphology by freeze-substitution and freeze-fracture and for electron probe x-ray microanlysis of sudden calcium displacement from intracellular stores in freeze-dried cryosections, all in the same fiber. We now show the feasibility and instrumentation of new methodology for stimulating a single, intact skeletal muscle fiber at a point resulting in the propagation of an action potential, followed by quick-freezing with sub-millisecond temporal resolution after electrical stimulation, followed by multiple sampling of the frozen muscle fiber for freeze-substitution, freeze-fracture (not shown) and cryosectionmg. This model, at once serving as its own control and obviating consideration of variances between different fibers, frogs etc., is useful to investigate structural and topochemical alterations occurring in the wake of an action potential.

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