Electron microscopic observations on pulmonary connective tissue stained by Ruthenium Red

1982 ◽  
Vol 14 (2) ◽  
pp. 257-271 ◽  
Author(s):  
Kimihiko Takusagawa ◽  
Fumio Ariji ◽  
Kuniji Shida ◽  
Toshio Sato ◽  
Noboru Asoo ◽  
...  
Keyword(s):  
Connective Tissue ◽  
Ruthenium Red ◽  
Electron Microscopic ◽  
Pulmonary Connective Tissue

Branching morphogenesis in the avian lung: electron microscopic studies using cationic dyes

Development ◽  
1986 ◽  
Vol 94 (1) ◽  
pp. 189-205
Author(s):  
Betty C. Gallagher
Keyword(s):  
Tannic Acid ◽  
Basal Lamina ◽  
Branching Morphogenesis ◽  
Ruthenium Red ◽  
Interparticle Spacing ◽  
Blue Staining ◽  
Mouse Lung ◽  
Alcian Blue Staining ◽  
Electron Microscopic ◽  
Avian Lung

The developing chick lung was examined in the electron microscope for intimate cell contacts between epithelium and mesenchyme, discontinuities in the basal lamina and substructure of the basement membrane. Cell filopodia were seen which crossed the basal lamina from both the epithelial and the mesenchymal cells. Ruthenium red and tannic acid staining of the basal lamina of the chick lung showed it to be thin and sometimes discontinuous at the tips compared to the more substantial basal lamina in the interbud areas. The bilaminar distribution of particles seen with ruthenium red is similar to those seen in the cornea and lens. With tannic acid staining, filaments could be seen which crossed the lamina lucida and connected with the lamina densa. Spikes perpendicular to the basal lamina were sometimes seen with a periodicity of approximately 110 nm. Alcian blue staining revealed structure similar to that seen by ruthenium red staining in the salivary and mammary glands, although the interparticle spacing was closer. Collagen was located in areas of morphogenetic stability, as has been seen by other investigators in different tissues. Collagen was coated with granules (probably proteoglycan) at periodic intervals when stained with ruthenium red. The fibrils were oriented circumferentially around the mesobronchus and were assumed to continue into the bud, but the fibres curve laterally at the middle of a bud. This orientation is opposite to that seen by another investigator in the mouse lung. In general, the observations made in the avian lung are similar to those seen in branching mammalian tissue. It is likely, therefore, that the chick lung uses strategies in its morphogenesis that are similar to those that have been elucidated previously in developing mammalian organs.

Adhesion of the Boston ivy tendril

1977 ◽  
Vol 55 (8) ◽  
pp. 918-924 ◽  
Author(s):  
Anton G. Endress ◽  
William W. Thomson
Keyword(s):  
Cell Walls ◽  
Tactile Stimulation ◽  
Electron Microscopic Examination ◽  
Ruthenium Red ◽  
Contact Interface ◽  
Electron Microscopic ◽  
Colloidal Iron ◽  
Peripheral Cells ◽  
Adhesive Secretion ◽  
Stimulation Of

Tactile stimulation of Boston ivy tendrils results in the development of bilaterally symmetric discs which adhere to substrates in the vicinity of the tendrils. Our electron microscopic examination of the tendrils indicates that adhesive secretion occurs from the peripheral cells at the contact face of the discs. Cell walls in this region develop pockets which fill with adhesive and ultimately coalesce. In fully adherent discs, the adhesive occupies the region between the substrate and the cells as well as the intracellular regions between the peripheral cells. While a cuticle was present on immature discs, no cuticle-like material was observed at the contact interface of mature discs.Staining of the adhesive was enhanced by ruthenium red and potassium ferrocyanide treatments, and the adhesive bound both colloidal iron and thorium. These results indicated that the adhesive is possibly a mucopolysaccharide.

Morphology and ultrastructure of oral strains of Actinobacillus actinomycetemcomitans and Haemophilus aphrophilus

1980 ◽  
Vol 30 (2) ◽  
pp. 588-600
Author(s):  
S C Holt ◽  
A C Tanner ◽  
S S Socransky
Keyword(s):  
Electron Microscopy ◽  
Ruthenium Red ◽  
Actinobacillus Actinomycetemcomitans ◽  
Electron Microscopic ◽  
Transmission Electron ◽  
Large Numbers ◽  
Haemophilus Aphrophilus ◽  
Ruthenium Red Staining ◽  
Amorphous Surface ◽  
Scanning Electron

Selected human oral and nonoral strains of the genera Actinobacillus and Haemophilus were examined by transmission and scanning electron microscopy. The strains examined were morphologically identical to recognized Actinobacillus actinomycetemcomitans, Haemophilus aphrophilus, and Haemophilus paraphrophilus. By transmission electron microscopy, the cells were typically gram negative in morphology, with several strains possessing some extracellular ruthenium red-staining polymeric material. Numerous vesicular structures, morphologically identical to lipopolysaccharide vesicles, were seen to originate from and be continuous with the surface of the outer membrane. Large numbers of these vesicles were also found in the external environment. Scanning electron microscopic observations revealed that both actinobacilli and haemophili possessed surface projections and an amorphous surface material which connected and covered adjacent cells.

Characterization of the ryanodine receptor/channel of invertebrate muscle

1998 ◽  
Vol 274 (2) ◽  
pp. R494-R502 ◽  
Author(s):  
Kerry E. Quinn ◽  
Loriana Castellani ◽  
Karol Ondrias ◽  
Barbara E. Ehrlich
Keyword(s):  
Ryanodine Receptor ◽  
Single Channel ◽  
Microscopic Analysis ◽  
Ruthenium Red ◽  
Electron Microscopic ◽  
Release Channel ◽  
Single Channel Currents ◽  
Channel Currents ◽  
Bell Shaped Curve

Electron-microscopic analysis was used to show that invertebrate muscle has feetlike structures on the sarcoplasmic reticulum (SR) displaying the typical four-subunit appearance of the calcium (Ca2+) release channel/ryanodine receptor (RyR) observed in vertebrate skeletal muscle (K. E. Loesser, L. Castellani, and C. Franzini-Armstrong. J. Muscle Res. Cell Motil. 13: 161–173, 1992). SR vesicles from invertebrate muscle exhibited specific ryanodine binding and single channel currents that were activated by Ca2+, caffeine, and ATP and inhibited by ruthenium red. The single channel conductance of this invertebrate RyR was lower than that of the vertebrate RyR (49 and 102 pS, respectively). Activation of lobster and scallop SR Ca2+ release channel, in response to cytoplasmic Ca2+ (1 nM–10 mM), reflected a bell-shaped curve, as is found with the mammalian RyR. In contrast to a previous report (J.-H. Seok, L. Xu, N. R. Kramarcy, R. Sealock, and G. Meissner. J. Biol. Chem. 267: 15893–15901, 1992), our results show that regulation of the invertebrate and vertebrate RyRs is quite similar and suggest remarkably similar paths in these diverse organisms.

Zymogen Secreting Cells in the Hepatopancreatic Duct of Pagurus Bernhardus (Linnaeus, 1758) and Clibanarius Longitarsus (De Haan, 1849) (Anomura)

Crustaceana ◽  
1995 ◽  
Vol 68 (5) ◽  
pp. 616-628
Author(s):  
C. Manjulatha ◽  
D. Erri Babu
Keyword(s):  
Connective Tissue ◽  
Secretory Activity ◽  
Secretory Cells ◽  
Main Duct ◽  
Zymogen Granules ◽  
Electron Microscopic ◽  
Food Material ◽  
Acidic Polysaccharide ◽  
Microscopic Studies ◽  
Pagurus Bernhardus

AbstractHistological and histochemical observations reveal that there are two clusters of acidic polysaccharide secreting glands at the opening of the mouth into the oesophagus in Pagurus bernhardus and Clibanarius longitarsus. Below these there are structurally similar glands in the connective tissue of the oesophagus. The epithelial cells lining the hepatopancreatic main duct show secretory activity and they secrete acidophilic granular secretions, which are chemically similar to vertebrate pancreatic zymogen granules. Electron microscopic studies reveal the presence of secretory cells in the hepatopancreatic duct and the synthesis of zymogens within these cells. The time of release of these granular secretions synchronizes with the release of the food material into the midgut.

Ultrastructural morphology of the olfactory pathway for cerebrospinal fluid drainage in the rabbit

1986 ◽  
Vol 64 (3) ◽  
pp. 466-473 ◽  
Author(s):  
Stephanie S. Erlich ◽  
J. Gordon McComb ◽  
Shigeyo Hyman ◽  
Martin H. Weiss
Keyword(s):  
Cerebrospinal Fluid ◽  
Connective Tissue ◽  
Electron Microscopic Examination ◽  
Olfactory Pathway ◽  
Electron Microscopic ◽  
En Bloc ◽  
Major Pathway ◽  
Content Type ◽  
Csf Drainage ◽  
Fine Print

✓ Previous physiological studies indicate that the olfactory region serves as a major pathway for cerebrospinal fluid (CSF) drainage into the lymphatic system. The present study was undertaken to determine the ultrastructural characteristics of this egress route. New Zealand White rabbits received a single bolus injection of the tracer ferritin (MW 400,000) into both lateral ventricles in such a manner as not to raise the intraventricular pressure above the normal level. The animals were sacrificed via intracardiac perfusion of fixative between less than 12 minutes and 4 hours following injection. The cribriform region was removed en bloc, decalcified, sectioned coronally, and prepared for light and electron microscopic examination. The arachnoid, dura, and periosteum surrounding the fila olfactoria passing through the cribriform plate merge together and form the perineurium, which consists of multiple layers of loosely overlapping cells with widely separated junctions and few vesicles. The perineurium surrounding the olfactory filaments at the superficial submucosal level is only one cell thick. The subarachnoid space freely communicates with the perineural space surrounding each filament. No morphological barrier between the perineural space and the loose submucosal connective tissue was identified. Whether or not the perineurium was multi- or singlelayered, ferritin was noted in abundance between the loosely overlapping perineural cells and in the submucosal connective tissue. The distribution of ferritin at 12 minutes was similar to that at 4 hours; however, the quantity of ferritin was increased at 4 hours. These results indicate that no significant barrier to CSF drainage is present at the rabbit cribriform region and that CSF reaches the submucosal region rapidly via open pathways.

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