Immunoelectron microscopy of cell surface antigens: a quantitative analysis of antibody binding after different fixation protocols

1980 ◽  
Vol 12 (3) ◽  
pp. 349-361 ◽  
Author(s):  
W. Van Ewijk ◽  
R. C. Coffman ◽  
I. L. Weissman
Keyword(s):  
Quantitative Analysis ◽  
Cell Surface ◽  
Immunoelectron Microscopy ◽  
Surface Antigens ◽  
Antibody Binding ◽  
Cell Surface Antigens

scholarly journals Suramin inhibits antibody binding to cell surface antigens and disrupts complement-mediated mesangial cell lysis

2016 ◽  
Vol 132 (4) ◽  
pp. 224-234 ◽  
Author(s):  
Honglan Piao ◽  
Yuan Chi ◽  
Xiling Zhang ◽  
Zhen Zhang ◽  
Kun Gao ◽  
...  
Keyword(s):  
Cell Surface ◽  
Mesangial Cell ◽  
Surface Antigens ◽  
Cell Lysis ◽  
Antibody Binding ◽  
Cell Surface Antigens

scholarly journals A High-Throughput Hybridoma Selection Method Using Fluorometric Microvolume Assay Technology

2008 ◽  
Vol 13 (3) ◽  
pp. 210-217 ◽  
Author(s):  
Rozanne Lee ◽  
Mylinh Tran ◽  
Mark Nocerini ◽  
Meina Liang
Keyword(s):  
Cell Surface ◽  
High Throughput ◽  
Antigen Expression ◽  
Surface Antigens ◽  
Antibody Binding ◽  
Enzyme Linked Immunosorbent Assay ◽  
Cell Surface Antigens ◽  
Homogeneous Assay ◽  
Exquisite Specificity ◽  
Promising Type

Monoclonal antibodies (mAb) are not only useful reagents but also represent a promising type of therapeutics due to their high affinity and exquisite specificity for their antigens. A critical step in mAb generation is to identify antigen-specific antibodies. Although enzyme-linked immunosorbent assay (ELISA) has been broadly applied for antibody selection against secreted antigens, an inherent disadvantage for ELISA is the difficulty in identifying antibodies that recognize the native conformation of cell surface antigens. To overcome this drawback, the authors have developed a high-throughput cell-based antibody binding assay using fluorometric microvolume assay technology (FMAT). This method offers a homogeneous assay for detection of antibody binding to its antigen on the cell surface. To distinguish antibodies that bind to antigen on the cell surface from those that bind nonspecifically to cells, the binding is assessed using both antigen-expressing cells and related cells devoid of the antigen expression. This assay can detect antibodies at a concentration as low as 5 ng/mL and cell surface antigen as low as 9000 copies per cell. Results demonstrate that the FMAT method provides a sensitive and homogeneous assay to detect antibody binding to cell surface antigens and is amenable for high-throughput hybridoma selection. ( Journal of Biomolecular Screening 2008:210-217)

Quantitative analysis of rat Ig (sub) classes binding to cell surface antigens

1982 ◽  
Vol 55 (2) ◽  
pp. 179-191 ◽  
Author(s):  
Rune Nilsson ◽  
Thomas Brodin ◽  
Hans-Olov Sjögren
Keyword(s):  
Quantitative Analysis ◽  
Cell Surface ◽  
Surface Antigens ◽  
Cell Surface Antigens
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