Contribution of organic and inorganic osmolytes to volume regulation in rat brain cells in culture

1993 ◽  
Vol 18 (4) ◽  
pp. 445-452 ◽  
Author(s):  
H. Pasantes-Morales ◽  
S. Alavez ◽  
R. S�nchez Olea ◽  
J. Mor�n
Keyword(s):  
Rat Brain ◽  
Volume Regulation ◽  
Brain Cells ◽  
Cells In Culture

scholarly journals Immunoreactive insulin from mouse brain cells in culture and whole rat brain

1984 ◽  
Vol 218 (1) ◽  
pp. 19-27 ◽  
Author(s):  
N P Birch ◽  
D L Christie ◽  
A G C Renwick
Keyword(s):  
Rat Brain ◽  
Mouse Brain ◽  
Culture Medium ◽  
Calf Serum ◽  
Brain Cells ◽  
Immunoreactive Insulin ◽  
Foetal Calf Serum ◽  
Cells In Culture ◽  
Pancreatic Insulin ◽  
Contrast Microscopy

Foetal mouse brain cells were cultured as described previously [Sotelo, Gibbs, Gajdusek, Toh & Wurth (1980) Proc. Natl. Acad. Sci. U.S.A. 77, 653-657] without added insulin and without foetal calf serum after 12 days in culture. Examination by phase-contrast microscopy showed that these modifications did not appear to affect growth and development of the cells adversely. Silver impregnation of the cultures and indirect immunofluorescence following reaction with tetanus toxin showed that a high proportion of the cells resembled neurones. Analysis of concentrated culture medium by radioimmunoassay and high-pressure liquid chromatography (h.p.l.c.) revealed that the cells produced two main forms of immunoreactive insulin which differed from authentic pancreatic insulin in retention time. Immunoreactive somatostatin was also produced in culture and this was resolved into at least three forms by h.p.l.c. Immunoreactive insulin was also extracted from whole rat brain by using two published procedures. The method of Havrankova, Schmechel, Roth & Brownstein [Proc. Natl. Acad. Sci. U.S.A. (1978) 75, 5737-5741] consistently gave greater yields of insulin than did that of Eng & Yalow [Diabetes (1980) 29, 105-109] and the concentration was about three times that of plasma. The extracted insulin was further characterized by h.p.l.c. in each case and was found to behave like authentic pancreatic insulin. The production of insulin and somatostatin by foetal mouse brain cells in culture suggests that they may be a useful model system for studies of neuropeptide biosynthesis.

Thyroid Hormone Action on Biosynthesis of Somatostatin by Fetal Rat Brain Cells in Culture*

Endocrinology ◽  
1988 ◽  
Vol 123 (2) ◽  
pp. 898-904 ◽  
Author(s):  
MARIA TERESA DE LOS FRAILES ◽  
LUCINDA CACICEDO ◽  
MARIA JESUS LORENZO ◽  
GUMERSINDO FERNANDEZ ◽  
FRANCO SANCHEZ-FRANCO
Keyword(s):  
Thyroid Hormone ◽  
Rat Brain ◽  
Brain Cells ◽  
Hormone Action ◽  
Cells In Culture ◽  
Thyroid Hormone Action ◽  
Fetal Rat ◽  
Fetal Rat Brain

Role of Locally Produced Growth Hormone-Releasing Factor in Somatostatin Regulation by Fetal Rat Brain Cells in Culture

1992 ◽  
Vol 55 (2) ◽  
pp. 221-229 ◽  
Author(s):  
Maria Teresa de los Frailes ◽  
Luanda Cacicedo ◽  
Gumersindo Fernandez ◽  
Rosa Maria Tolón ◽  
Maria Jesus Lorenzo ◽  
...  
Keyword(s):  
Growth Hormone ◽  
Rat Brain ◽  
Brain Cells ◽  
Growth Hormone Releasing Factor ◽  
Cells In Culture ◽  
Fetal Rat ◽  
Fetal Rat Brain

scholarly journals Effects of methyl isocyanate on rat brain cells in culture.

1990 ◽  
Vol 47 (9) ◽  
pp. 596-601
Author(s):  
D Anderson ◽  
S Goyle ◽  
B J Phillips ◽  
A Tee ◽  
L Beech ◽  
...  
Keyword(s):  
Rat Brain ◽  
Brain Cells ◽  
Methyl Isocyanate ◽  
Cells In Culture

Chemically evoked release of an angiotensin-like peptide from fetal rat brain cells in culture

1986 ◽  
Vol 251 (1) ◽  
pp. C17-C22 ◽  
Author(s):  
J. M. Meyer ◽  
J. A. Weyhenmeyer
Keyword(s):  
Rat Brain ◽  
High Performance ◽  
Renin Angiotensin System ◽  
Brain Cells ◽  
Ang Ii ◽  
Sprague Dawley ◽  
Cells In Culture ◽  
Fetal Rat ◽  
Presynaptic Nerve Terminals ◽  
Fetal Rat Brain

Recent evidence has demonstrated that dissociated fetal rat brain cells in culture synthesize an angiotensin II (ANG II)-like peptide that shares common properties with authentic ANG II, suggesting that brain has a complete renin angiotensin system that is independent of peripheral substrates. Although ANG II has been postulated to function as a neurotransmitter/neuromodulator, the release of ANG II from presynaptic nerve terminals has not been established. To investigate the mechanism of ANG II release, brain cells from 20-day gestational age Sprague-Dawley rats were dissociated by mild trypsinization and grown in culture. The cells were maintained in serum-free medium for 5 days prior to experimental analysis. Cultured brain cells were challenged with 59 mM KCl in the presence or absence of 5 mM CaCl2, and the incubation medium was measured for the release of ANG II by radioimmunoassay and high-performance liquid chromatography. The data demonstrate that K+ stimulation results in a rapid and time-dependent release of ANG II-like peptide that is Ca2+ dependent. We have concluded that these findings are consistent with those for other neurotransmitter/neurohormone systems and therefore provide further support for the role of ANG II as a chemical transmitter in the brain.

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