In vitro and in vivo isolation ofLeishmania tropica from Saudi Arabia

1985 ◽  
Vol 71 (2) ◽  
pp. 271-272 ◽  
Author(s):  
A. A. Mahmoud ◽  
A. Al-Tuwaijri ◽  
I. Al-Mofleh ◽  
S. A. Al-Khuwaitir
Keyword(s):  
Saudi Arabia

Chara braunii (Charales, Charophyta) in an Arid Rainfed Waterbody, Saudi Arabia

1999 ◽  
Vol 47 (3) ◽  
pp. 427 ◽  
Author(s):  
M. I. Hussain ◽  
T. M. Khoja
Keyword(s):  
Saudi Arabia ◽  
Aquatic Vegetation ◽  
Gradual Increase ◽  
Water Level Fluctuations ◽  
Salinity Range ◽  
Concentration Of Ions ◽  
Temporal Succession ◽  
Vegetation Water

An account is given of a typical rainfed waterbody in the Al-Ammariyah Wadi (Saudi Arabia), with special reference to charophyte vegetation, water chemistry and topography of the area, which was studied from April 1996 to July 1997. Recurring patterns following rainfall and inundation of the waterbody are described as a model of temporal succession of biotic communities. Unispecific Chara braunii Gm. meadows were the first aquatic vegetation to emerge and overwhelmingly dominated the freshwater lentic ecosystem. This was followed by plankton and desert plants as the waterbody dried out. Chara braunii is reported as a new record for the Saudi Arabian charoflora. The species is characterised as stenohaline and tends to grow in vivo and in vitro in the salinity range of 0.2–0.8‰. A gradual increase in elements and ions (Si 20–31 mg L–1 and pH 6.8–7.6) in the water was demonstrated as the waterbody desiccated. As a result of the increasing concentration of ions and pH, C. braunii developed heavy encrustation, and hastened fructification prior to desiccation of the waterbody between June and July 1997. Survival and emergence of C. braunii is positively correlated with drought resistant-oospores, specificity to hyposalinity, water-level fluctuations, and absence of herbivores.

scholarly journals An Efficient and Easy Micro-propagation of Reseda Lutea (Resedaceae) a Rare and Medicinally Valuable Plant of Saudi Arabia

2021 ◽  
Author(s):  
FAHAD AL-QURAINY ◽  
SALEH ALANSI ◽  
SALIM KHAN ◽  
MOHAMMAD NADEEM ◽  
AREF AL-SHAMERI ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Saudi Arabia ◽  
Peat Moss ◽  
Ms Medium ◽  
Root Initiation ◽  
Adventitious Buds ◽  
Micro Propagation ◽  
Complete Procedure

Abstract The goal of this work was to look at the propagation of Reseda lutea L. by organogenesis in tissue culture. Explants from in vitro grown seedlings were taken from the axillary bud. After seven days of culture on MS medium supplemented with 1.0 mg/l BA, the adventitious buds developed. After three weeks of culturing on MS medium supplemented with 1.5 mg/l BA, the maximum multiplication of shoots (16.12 shoots/explant) was discovered, with an average (7.37 cm) shoots/explant. These shoots were sub-cultured on MS media with varying concentrations of NAA and IBA for root initiation. The MS medium combined with IBA produced the greatest percentage of root development (92%) and the greatest number of roots (7.37 roots/plant). In MS media supplemented with 0.5 NAA, the longest roots (3.08 cm) were found. After 17 days in a glasshouse, the plantlets were acclimatized in pots containing Peat moss and pearlite, 98 percent of the plantlets were acclimatized. To get a plant in a pot, the complete procedure took about 75 days. The technique proposed could aid in the preservation of the plant both in vivo and in vitro.

In vitro and in vivo study of cucurbitacins-type triterpene glucoside from Citrullus colocynthis growing in Saudi Arabia against hepatocellular carcinoma

2012 ◽  
Vol 33 (2) ◽  
pp. 245-251 ◽  
Author(s):  
Seif-Eldin N. Ayyad ◽  
Ahmed Abdel-Lateff ◽  
Walied M. Alarif ◽  
Francesca R. Patacchioli ◽  
Farid A. Badria ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Saudi Arabia ◽  
In Vivo Study ◽  
Citrullus Colocynthis

scholarly journals In vitro and in vivo isolation ofLeishmania tropica from Saudi Arabia

1986 ◽  
Vol 72 (1) ◽  
pp. 141-141
Author(s):  
A. A. Mahmoud ◽  
A. Al-Tuwaijri ◽  
I. Al-Mofleh ◽  
S. A. Al-Khuwaitir
Keyword(s):  
Saudi Arabia

Induction and Differentiation of Dental Epithelium in Vivo and in Vitro

1982 ◽  
Vol 40 ◽  
pp. 142-145
Author(s):  
E. J. Kollar
Keyword(s):  
Connective Tissue ◽  
Oral Mucosa ◽  
Basal Lamina ◽  
Functional Derivatives ◽  
Specific Source ◽  
Dental Epithelium ◽  
Connective Tissue Stroma

The differentiation and maintenance of many specialized epithelial structures are dependent on the underlying connective tissue stroma and on an intact basal lamina. These requirements are especially stringent in the development and maintenance of the skin and oral mucosa. The keratinization patterns of thin or thick cornified layers as well as the appearance of specialized functional derivatives such as hair and teeth can be correlated with the specific source of stroma which supports these differentiated expressions.

Ultrastructural Correlations between Clonal Human Tumor Colonies and in Vivo Neoplasms

1982 ◽  
Vol 40 ◽  
pp. 210-211
Author(s):  
M.J. Murphy ◽  
R.R. Price ◽  
J.C. Sloman
Keyword(s):  
Cultured Cells ◽  
Human Tumor ◽  
Cell Type ◽  
Tumor Mass ◽  
Initial Cell ◽  
Cloning Assay ◽  
Human Tumor Cloning ◽  
The Relationship

The in vitro human tumor cloning assay originally described by Salmon and Hamburger has been applied recently to the investigation of differential anti-tumor drug sensitivities over a broad range of human neoplasms. A major problem in the acceptance of this technique has been the question of the relationship between the cultured cells and the original patient tumor, i.e., whether the colonies that develop derive from the neoplasm or from some other cell type within the initial cell population. A study of the ultrastructural morphology of the cultured cells vs. patient tumor has therefore been undertaken to resolve this question. Direct correlation was assured by division of a common tumor mass at surgical resection, one biopsy being fixed for TEM studies, the second being rapidly transported to the laboratory for culture.

Ultrastructure of melanocyte-keratinocyte interactions and pigment transfer in vitro

1978 ◽  
Vol 36 (2) ◽  
pp. 650-651
Author(s):  
Raul I. Garcia ◽  
Evelyn A. Flynn ◽  
George Szabo
Keyword(s):  
Direct Injection ◽  
Skin Pigmentation ◽  
Freeze Fracture ◽  
Pigment Granule ◽  
Time Lapse ◽  
Ultrastructural Level ◽  
Lanthanum Tracer ◽  
A Cell

Skin pigmentation in mammals involves the interaction of epidermal melanocytes and keratinocytes in the structural and functional unit known as the Epidermal Melanin Unit. Melanocytes(M) synthesize melanin within specialized membrane-bound organelles, the melanosome or pigment granule. These are subsequently transferred by way of M dendrites to keratinocytes(K) by a mechanism still to be clearly defined. Three different, though not necessarily mutually exclusive, mechanisms of melanosome transfer have been proposed: cytophagocytosis by K of M dendrite tips containing melanosomes, direct injection of melanosomes into the K cytoplasm through a cell-to-cell pore or communicating channel formed by localized fusion of M and K cell membranes, release of melanosomes into the extracellular space(ECS) by exocytosis followed by K uptake using conventional phagocytosis. Variability in methods of transfer has been noted both in vivo and in vitro and there is evidence in support of each transfer mechanism. We Have previously studied M-K interactions in vitro using time-lapse cinemicrography and in vivo at the ultrastructural level using lanthanum tracer and freeze-fracture.

In vitro and in vivo polysaccharides assembly: ultrastructural and cytochemical study of growing plant cell wall components.

1978 ◽  
Vol 36 (2) ◽  
pp. 434-435
Author(s):  
D. Reis ◽  
B. Vian ◽  
J. C. Roland
Keyword(s):  
Cell Wall ◽  
Self Assembly ◽  
Plant Cell Wall ◽  
Higher Plants ◽  
Three Dimensional ◽  
Cytochemical Study ◽  
Wall Components

Wall morphogenesis in higher plants is a problem still open to controversy. Until now the possibility of a transmembrane control and the involvement of microtubules were mostly envisaged. Self-assembly processes have been observed in the case of walls of Chlamydomonas and bacteria. Spontaneous gelling interactions between xanthan and galactomannan from Ceratonia have been analyzed very recently. The present work provides indications that some processes of spontaneous aggregation could occur in higher plants during the formation and expansion of cell wall.Observations were performed on hypocotyl of mung bean (Phaseolus aureus) for which growth characteristics and wall composition have been previously defined.In situ, the walls of actively growing cells (primary walls) show an ordered three-dimensional organization (fig. 1). The wall is typically polylamellate with multifibrillar layers alternately transverse and longitudinal. Between these layers intermediate strata exist in which the orientation of microfibrils progressively rotates. Thus a progressive change in the morphogenetic activity occurs.

Probing the ultrastructure of the mitotic and meiotic spindle in eggs: An expeditious approach based on semi-thin (0.25 μm) serial sections

1983 ◽  
Vol 41 ◽  
pp. 552-555
Author(s):  
Conly L. Rieder ◽  
S. Bowser ◽  
R. Nowogrodzki ◽  
K. Ross ◽  
G. Sluder
Keyword(s):  
Small Sample Size ◽  
Small Sample ◽  
Meiotic Spindle ◽  
Serial Sections ◽  
Mitotic Apparatus ◽  
Thin Sections ◽  
Cell Cycles ◽  
Ultrastructural Studies

Eggs have long been a favorite material for studying the mechanism of karyokinesis in-vivo and in-vitro. They can be obtained in great numbers and, when fertilized, divide synchronously over many cell cycles. However, they are not considered to be a practical system for ultrastructural studies on the mitotic apparatus (MA) for several reasons, the most obvious of which is that sectioning them is a formidable task: over 1000 ultra-thin sections need to be cut from a single 80-100 μm diameter egg and of these sections only a small percentage will contain the area or structure of interest. Thus it is difficult and time consuming to obtain reliable ultrastructural data concerning the MA of eggs; and when it is obtained it is necessarily based on a small sample size.We have recently developed a procedure which will facilitate many studies concerned with the ultrastructure of the MA in eggs. It is based on the availability of biological HVEM's and on the observation that 0.25 μm thick serial sections can be screened at high resolution for content (after mounting on slot grids and staining with uranyl and lead) by phase contrast light microscopy (LM; Figs 1-2).

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