Enhancement of interleukin-2 and ?-interferon productionin vitro on cord blood lymphocytes andin vivo on primary cellular immunodeficiency patients with thymic extract (Thymostimulin)

1988 ◽  
Vol 8 (2) ◽  
pp. 103-107 ◽  
Author(s):  
Ching-Yuang Lin ◽  
Yuh-Chi Kuo ◽  
Chiou-Chyn Lin ◽  
Bor-Rung Ou
Keyword(s):  
Cord Blood ◽  
Interleukin 2 ◽  
Blood Lymphocytes ◽  
Thymic Extract ◽  
Cellular Immunodeficiency ◽  
Cord Blood Lymphocytes

scholarly journals Expression of the interleukin-2 receptor gamma chain on cord blood mononuclear cells

Blood ◽  
1996 ◽  
Vol 87 (8) ◽  
pp. 3344-3350 ◽  
Author(s):  
S Saito ◽  
T Morii ◽  
H Umekage ◽  
K Makita ◽  
K Nishikawa ◽  
...  
Keyword(s):  
T Cells ◽  
Cord Blood ◽  
Nk Cells ◽  
Mononuclear Cells ◽  
Interleukin 2 ◽  
Gamma Chain ◽  
Blood Lymphocytes ◽  
Interleukin 2 Receptor ◽  
Significant Difference ◽  
Cord Blood Lymphocytes

Lymphocytes in umbilical cord blood and neonatal peripheral blood have been shown to have less ability in an immune reaction. In our present experimental approach to address this issue, we made use of the cord blood of full-term birth infants to investigate the expression of the interleukin- 2 receptor gamma (IL-2Rgamma) chain that is shared with receptors for IL-4, IL-7, IL-9, and IL-15 as well as IL-2. The gamma chain expression in cord blood lymphocytes was about one-third that in the lymphocytes of adults, whereas no significant difference between cord blood and adult monocytes was observed. A reduced expression of the gamma chain was observed in all of the CD4+ T cells, CD8+ T cells, gamma-delta T cells, B cells, CD16+ natural killer (NK) cells, and CD56(bright) NK cells of the cord blood lymphocytes. The reduced gamma chain expression reached two-thirds of that in adults after 3 days of culture in vitro and in infants 3 days after birth, thus implying that the increase in the gamma chain may significantly contribute to the prevention of neonatal infection.

Infusion of in vivo expanded cord blood lymphocytes: A new strategy to control residual disease?

2018 ◽  
Vol 66 (3) ◽  
pp. 91-93 ◽  
Author(s):  
S. Lamure ◽  
J. Delage ◽  
L. Vincent ◽  
N. Fegueux ◽  
L. Zhao-Yang ◽  
...  
Keyword(s):  
Cord Blood ◽  
Residual Disease ◽  
Blood Lymphocytes ◽  
New Strategy ◽  
Cord Blood Lymphocytes

A population of human cord blood lymphocytes which generates Fc receptors in vitro

1977 ◽  
Vol 30 (1) ◽  
pp. 54-65 ◽  
Author(s):  
S. Witemeyer ◽  
A.D. Bankhurst ◽  
R.C. Williams
Keyword(s):  
Cord Blood ◽  
Fc Receptors ◽  
Human Cord Blood ◽  
Blood Lymphocytes ◽  
Cord Blood Lymphocytes

scholarly journals Treatment of Pregnant Women with a Betamimetic and Verapamil Increases the Micronuclei Frequency in Umbilical Cord Blood Lymphocytes

2008 ◽  
Vol 215 (4) ◽  
pp. 363-371 ◽  
Author(s):  
Darko Grujicic ◽  
Olivera Miloševic-Djordjevic ◽  
Slobodan Arsenijevic ◽  
Dragoslav Marinkovic
Keyword(s):  
Cord Blood ◽  
Pregnant Women ◽  
Umbilical Cord ◽  
Umbilical Cord Blood ◽  
Blood Lymphocytes ◽  
Cord Blood Lymphocytes

In vivo Activated Cord Blood Lymphocytes Express High Affinity lnterleukin-2 Receptor: Evaluation of Their Responsiveness to in vitro Stimulation with Recombinant lnterleukin-2

Neonatology ◽  
1992 ◽  
Vol 62 (6) ◽  
pp. 385-394 ◽  
Author(s):  
D. Montagna ◽  
A. Moretta ◽  
M. Marconi ◽  
G. Mingrat ◽  
A. Gasparoni ◽  
...  
Keyword(s):  
Cord Blood ◽  
Blood Lymphocytes ◽  
High Affinity ◽  
Cord Blood Lymphocytes

Intracellular Cytokine Profile of Cord and Adult Blood Lymphocytes

Blood ◽  
1998 ◽  
Vol 92 (1) ◽  
pp. 11-18 ◽  
Author(s):  
I.M.H. Chalmers ◽  
G. Janossy ◽  
M. Contreras ◽  
C. Navarrete
Keyword(s):  
Flow Cytometry ◽  
Cord Blood ◽  
Mononuclear Cells ◽  
Interleukin 2 ◽  
Peripheral Blood Lymphocytes ◽  
Cytokine Profile ◽  
Color Flow ◽  
Blood Lymphocytes ◽  
Tnf Α ◽  
Ifn Γ

Umbilical cord blood (CB) transplantation is thought to be associated with a reduced risk of severe graft-versus-host-disease (GVHD) compared with bone marrow transplantation (BMT). The cytokine cascade is known to be important in the pathogenesis of GVHD; however, previous studies investigating the cytokine secretion pattern of CB cells have been contradictory because of variations in experimental techniques. In this study, the cytokine profile of cord and adult blood lymphocytes and lymphocyte subsets has been assessed at the single-cell level by flow cytometry, using CD4/CD8 and CD45RA/CD45RO markers. Cord and adult blood mononuclear cells were stimulated with phorbol 12-myristate 13-acetate (PMA) and ionomycin in the presence of monensin. After 4 to 24 hours of incubation, interleukin-2 (IL-2), IL-4, interferon-γ (IFN-γ), and tumor necrosis factor-α (TNF-α) production was measured by three-color flow cytometry. The results show that cord blood lymphocytes (CBL) produce less IL-2, IL-4, IFN-γ, and TNF-α than adult peripheral blood lymphocytes (ABL). Further subset analysis showed that in CBL the majority of cytokine producing cells were CD4+CD45RA+, whereas in ABL the cytokine-producing cells were both CD4+CD45RO+ and CD8+CD45RO+. These results suggest that the reduced incidence of GVHD in CB transplantation may partly due to the altered cytokine profile seen in CBL.

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