Differential effects of reactive oxygen species on native synovial fluid and purified human umbilical cord hyaluronate

Inflammation ◽  
1993 ◽  
Vol 17 (4) ◽  
pp. 403-415 ◽  
Author(s):  
Herkko Saari ◽  
Yrj� T. Konttinen ◽  
Claes Friman ◽  
Timo Sorsa
Keyword(s):  
Reactive Oxygen Species ◽  
Synovial Fluid ◽  
Umbilical Cord ◽  
Reactive Oxygen ◽  
Human Umbilical Cord ◽  
Oxygen Species ◽  
Differential Effects

scholarly journals Differential effects of Bcl-2 and caspases on mitochondrial permeabilization during endogenous or exogenous reactive oxygen species-induced cell death

2012 ◽  
Vol 28 (4) ◽  
pp. 239-253 ◽  
Author(s):  
Vincent Rincheval ◽  
Marie Bergeaud ◽  
Lise Mathieu ◽  
Jacqueline Leroy ◽  
Arnaud Guillaume ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cell Death ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Differential Effects ◽  
Mitochondrial Permeabilization

scholarly journals Differential Effects of Reactive Oxygen Species on IgG versus IgM Levels in TLR-Stimulated B Cells

2020 ◽  
Vol 204 (8) ◽  
pp. 2133-2142 ◽  
Author(s):  
Karin Margaretha Gilljam ◽  
Kristine Lillebø Holm ◽  
Muhammad Zahoor ◽  
Federica Grazia Centonze ◽  
Hesso Farhan ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
B Cells ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Differential Effects

scholarly journals Reactive oxygen species rapidly increase endothelial ICAM-1 ability to bind neutrophils without detectable upregulation

Blood ◽  
1994 ◽  
Vol 83 (9) ◽  
pp. 2669-2677
Author(s):  
H Sellak ◽  
E Franzini ◽  
J Hakim ◽  
C Pasquier
Keyword(s):  
Reactive Oxygen Species ◽  
Sialic Acid ◽  
Platelet Activating Factor ◽  
Reactive Oxygen ◽  
Polymorphonuclear Neutrophils ◽  
Intercellular Adhesion Molecule ◽  
Human Umbilical Cord ◽  
Oxygen Species ◽  
Adhesion Properties ◽  
Dependent Mechanism

We compared the effects of phorbol 12-myristate 13-acetate (PMA) and thrombin with those of nonlytic concentrations of reactive oxygen species (ROS) generated by hypoxanthine (HX)-xanthine oxidase (XO) on the adhesion properties of human umbilical cord vein endothelial cells (HUVEC) to resting polymorphonuclear neutrophils (PMN). PMN adherence to HX-XO-treated HUVEC was increased approximately twofold to 2.5-fold relative to untreated HUVEC, both immediately and after 2 hours. It was not additive to that induced by PMA or thrombin stimulation of HUVEC. ROS-induced adherence was not due to platelet-activating factor (PAF) or P-selectin expression, as it was neither antagonized by BN52021 (PAF receptor antagonist) nor inhibited by anti-P-selectin monoclonal antibody (MoAb), contrary to the increased adhesion of PMA- and thrombin-stimulated HUVEC. PMN preincubated with mannose-6-P or N- acetylneuraminic acid (sialic acid), but not mannose or galactose-6-P, showed reduced adherence to ROS-treated HUVEC, suggesting that carbohydrate molecules were expressed on the latter and served as the ligand for the PMN L-selectin. Intercellular adhesion molecule (ICAM- 1), constitutively present on the surface of resting HUVEC, was involved in the PMN adherence to ROS-treated HUVEC, since this adherence was inhibited by anti-ICAM-1, anti-CD11a, anti-CD11b, and anti-CD18 MoAbs. A non-CD18, non-ICAM-1-dependent mechanism is also involved in this adherence, since effects of these MoAbs were not additive; moreover, combinations of anti-CD18 and anti-ICAM-1 MoAbs with mannose-6-P and sialic acid completely inhibited PMN adherence. The increased binding of PMN to HX-XO-exposed HUVEC observed here involved IC-AM-1, but was independent of its upregulation, and another non-ICAM-1-dependent mechanism, in which carbohydrates expressed on HUVEC recognize L-selectin on PMN.

scholarly journals Do blood components affect the production of reactive oxygen species (ROS) by equine synovial cells in vitro?

2012 ◽  
Vol 32 (12) ◽  
pp. 1355-1360 ◽  
Author(s):  
Patrícia M. Brossi ◽  
Raquel Y.A. Baccarin ◽  
Cristina O. Massoco
Keyword(s):  
Reactive Oxygen Species ◽  
Synovial Fluid ◽  
Blood Product ◽  
Musculoskeletal Diseases ◽  
Reactive Oxygen ◽  
Synovial Cells ◽  
Oxygen Species ◽  
Synovial Fluid Cells ◽  
Antioxidant Effects

Blood-derived products are commonly administered to horses and humans to treat many musculoskeletal diseases, due to their potential antioxidant and anti-inflammatory effects. Nevertheless, antioxidant effects have never been shown upon horse synovial fluid cells in vitro. If proved, this could give a new perspective to justify the clinical application of blood-derived products. The aim of the present study was to investigate the antioxidant effects of two blood-derived products - plasma (unconditioned blood product - UBP) and a commercial blood preparation (conditioned blood product - CBP)¹ - upon stimulated equine synovial fluid cells. Healthy tarsocrural joints (60) were tapped to obtain synovial fluid cells; these cells were pooled, processed, stimulated with lipopolysaccharide (LPS) or phorbol 12-myristate 13-acetate (PMA), and evaluated by flow cytometry for the production of reactive oxygen species (ROS). Upon addition of any blood-derived product here used - UBP and CBP - there was a significant decrease in the oxidative burst of synovial fluid cells (P<0.05). There was no difference between UBP and CBP effects. In conclusion, treatment of stimulated equine synovial cells with either UBP or CBP efficiently restored their redox equilibrium.

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