Action of blood plasma and bone marrow extracts from leukemia patients on cultures of leucocytes from normal blood

1956 ◽  
Vol 41 (5) ◽  
pp. 428-430
Author(s):  
E. I. Terentyeva
Keyword(s):  
Bone Marrow ◽  
Blood Plasma ◽  
Normal Blood

scholarly journals Higher oxidation and lower antioxidant levels in peripheral blood plasma and bone marrow plasma from advanced cancer patients

Cancer ◽  
2002 ◽  
Vol 94 (12) ◽  
pp. 3247-3251 ◽  
Author(s):  
Elena M. V. de Cavanagh ◽  
Alba E. Honegger ◽  
Erica Hofer ◽  
Raul H. Bordenave ◽  
Eduardo O. Bullorsky ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Blood Plasma ◽  
Cancer Patients ◽  
Advanced Cancer ◽  
Peripheral Blood ◽  
Advanced Cancer Patients ◽  
Bone Marrow Plasma ◽  
Peripheral Blood Plasma

scholarly journals Phenotypic heterogeneity of TDT+ cells in the blood and bone marrow: implications for surveillance of residual leukemia [see comments]

Blood ◽  
1989 ◽  
Vol 74 (1) ◽  
pp. 312-319
Author(s):  
RG Smith ◽  
RL Kitchens
Keyword(s):  
Bone Marrow ◽  
Residual Disease ◽  
Lymphoblastic Leukemia ◽  
Leukemic Cells ◽  
Leukemia Cells ◽  
Normal Blood ◽  
Terminal Deoxynucleotidyl Transferase ◽  
Hla Dr ◽  
Lineage Markers ◽  
Minimal Residual

Terminal deoxynucleotidyl transferase (TdT) is a useful marker for normal lymphocyte precursors and acute lymphoblastic leukemia (ALL). Our previous studies, however, have shown that for monitoring minimal residual disease in the circulation, assay for TdT alone is not sufficiently specific to distinguish leukemia cells from the background of rare normal blood TdT+ cells. In an attempt to increase specificity for leukemic cells, we have used double and triple immunophenotypic analysis to characterize normal circulating and bone marrow TdT+ cells. Overall, normal TdT+ cells were about 1000-fold more frequent in the marrow than in the blood. More than 75% of TdT+ cells in both the blood and marrow expressed the CD34, CD22, and HLA-DR antigens. However, circulating TdT+ cells infrequently expressed CD19 (4.5%) and CD9 (2.3%), compared with their marrow counterparts (74% and 47%, respectively). The brightly staining CD10+ phenotype, frequently associated with ALL blasts, was significantly less common among normal blood (5.7%) than marrow (31%) TdT+ cells. Although T-lineage markers were rarely expressed on TdT+ cells in either site, CD7+ cells were far more prevalent within the circulating TdT+ subset (4%) than among the marrow population (less than 0.2%). The results suggest a selective release of lineage-uncommitted and/or thymus-destined TdT+ cells from the marrow into the circulation. Moreover, since CD19, CD9, and high- density CD10 are frequently found on ALL blasts, staining for these markers on TdT+ cells in the circulation should improve the specificity of assay for residual common ALL cells. Likewise, assay for CD5+ and possibly CD7+ TdT+ cells in either marrow or blood should provide a very sensitive method of detection of T-ALL blasts.

IN VITRO UPTAKE OF Fe59 BY BLOOD CELLS FROM NORMAL BIRDS AND BIRDS WITH ERYTHROBLASTOSIS

1963 ◽  
Vol 41 (1) ◽  
pp. 2057-2064 ◽  
Author(s):  
R. Bather ◽  
Stella Dzuibalo ◽  
C. le Q. Darcel
Keyword(s):  
Bone Marrow ◽  
Ammonium Sulphate ◽  
Blood Cells ◽  
Red Cells ◽  
Normal Blood ◽  
Promoting Effect ◽  
Heme Synthesis ◽  
Heat Resistant ◽  
In Vitro Uptake

The cellular elements of blood obtained from birds with virus-induced erythroblastosis rapidly incorporated radioactivity when incubated in vitro with Fe59Cl3. This effect was not seen until primitive red cells entered the circulating blood. Little of the iron that was incorporated reached heme. Instead most of it was retained by a heat-resistant component which was precipitable with 35% ammonium sulphate and had properties suggestive of a ferritin-like substance. Plasma from leukemic blood had a slight promoting effect on incorporation of Fe59 into normal blood cells in vitro when compared with normal plasma.Blood cells from birds in which the bone marrow was rendered hyperactive with phenylhydrazine also incorporated Fe59 in vitro, a large proportion of this reaching heme. Incorporation into the heme fraction was also observed in blood cells from erythroblastosis-infected birds treated with phenylhydrazine, indicating that heme synthesis was not completely blocked in these birds.

Sign in / Sign up

Export Citation Format

Share Document