Electron-microscopic autoradiographic study of DNA synthesis in tubular epithelial cells of the albino rat kidney with necrotozing nephrosis due to mercuric chloride

1987 ◽  
Vol 104 (2) ◽  
pp. 1162-1165
Author(s):  
V. P. Andreev ◽  
A. A. Pal'tsyn
Keyword(s):  
Epithelial Cells ◽  
Dna Synthesis ◽  
Mercuric Chloride ◽  
Rat Kidney ◽  
Autoradiographic Study ◽  
Tubular Epithelial Cells ◽  
Electron Microscopic ◽  
Albino Rat

Purpurogallin is a more powerful protector of kidney cells than Trolox and allopurinol

1992 ◽  
Vol 70 (8) ◽  
pp. 684-690 ◽  
Author(s):  
Ling-Hua Zeng ◽  
Tai-Wing Wu
Keyword(s):  
Xanthine Oxidase ◽  
Phase Contrast ◽  
Potent Inhibitor ◽  
Rat Kidney ◽  
Peroxyl Radicals ◽  
Kidney Cells ◽  
Tubular Epithelial Cells ◽  
Electron Microscopic ◽  
Oxidase Inhibition ◽  
Xanthine Oxidase Inhibition

Phase contrast and electron microscopic experiments demonstrated that oxyradicals generated with xanthine oxidase and hypoxanthine markedly damage rat kidney mesangial and porcine tubular epithelial cells. Purpurogallin, a phenol found in oak nutgalls, prolongs survival of the xanthine oxidase exposed renal cells three- to nine-fold longer than those without purpurogallin present. At levels equimolar to purpurogallin, either Trolox or allopurinol is less effective in delaying cell necrosis. Purpurogallin scavenges not only xanthine oxidase generated oxyradicals, but also non-enzymatically produced peroxyl radicals, more actively than equimolar levels of Trolox or allopurinol. Purpurogallin inhibits xanthine oxidase with severalfold higher potency than allopurinol and its more active metabolite oxypurinol. Therefore, purpurogallin is a stronger antioxidant than Trolox and a more potent inhibitor of xanthine oxidase than allopurinol as well as oxypurinol.Key words: purpurogallin, kidney cells, oxyradical damage, xanthine oxidase inhibition, antioxidant.

Adult-onset calorie restriction delays the accumulation of mitochondrial enzyme abnormalities in aging rat kidney tubular epithelial cells

2007 ◽  
Vol 292 (6) ◽  
pp. F1751-F1760 ◽  
Author(s):  
Susan H. McKiernan ◽  
Victoria C. Tuen ◽  
Katherine Baldwin ◽  
Jonathan Wanagat ◽  
Arjang Djamali ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Calorie Restriction ◽  
Rat Kidney ◽  
Mitochondrial Enzyme ◽  
Tubular Epithelial Cells ◽  
Renal Tubular Epithelial Cells ◽  
Deletion Mutations ◽  
Aging Rat ◽  
Mtdna Deletion ◽  
Renal Tubular

Adult-onset calorie restriction (A-CR) is an experimental model of life extension and healthy aging less explored, compared with calorie restriction begun at early ages, but one more realistic for human application. We examined the effect of A-CR on the aging rat kidney with respect to common structural age-dependent changes and the accumulation of mitochondrial enzyme abnormalities in tubular epithelial cells. A 40% calorie restriction was initiated in middle-aged rats, before the onset of significant age-related changes in the Fischer × Brown Norway rat kidney. This dietary intervention effectively reduced glomerulosclerosis and tubular atrophy within 6 mo and changed the rate of interstitial fibrosis formation within 1 yr and vascular wall thickening and the expression cytochrome c oxidase (COX)-deficient tubular epithelial cells in 18 mo compared with age-matched ad libitum-fed rats. Our histological approach (histochemical staining for mitochondrial enzyme activity and laser capture microdissection) coupled with mitochondrial DNA (mtDNA) PCR analyses demonstrated that COX-deficient renal tubular epithelial cells accumulated mtDNA deletion mutations and that these cells contained unique, clonally expanded mtDNA deletion mutations. Renal tubular epithelial cells with mitochondrial abnormalities presented cellular characteristics indicative of physiological dysfunction.

scholarly journals Fluorescent imaging of acute mercuric chloride exposure on cultured human kidney tubular epithelial cells

2004 ◽  
Vol 66 (6) ◽  
pp. 2279-2282 ◽  
Author(s):  
Zhichao Zhang ◽  
Xiangfeng Guo ◽  
Xuhong Qian ◽  
Z.H.E. Lu ◽  
Fengyu Liu
Keyword(s):  
Epithelial Cells ◽  
Mercuric Chloride ◽  
Human Kidney ◽  
Fluorescent Imaging ◽  
Tubular Epithelial Cells

An electron microscopic radioautographic study on the DNA synthesis of cecal epithelial cells in aging mice

1995 ◽  
Vol 28 (3-4) ◽  
pp. 213-217 ◽  
Author(s):  
Chunji Jin ◽  
Fulu Gao ◽  
Lin Sun ◽  
Hongjun Ma ◽  
Tetsuji Nagata
Keyword(s):  
Epithelial Cells ◽  
Dna Synthesis ◽  
Electron Microscopic

scholarly journals Light microscopic autoradiographic study of DNA synthesis in cecal epithelial cells of aging mice.

1995 ◽  
Vol 43 (12) ◽  
pp. 1223-1228 ◽  
Author(s):  
C Jin ◽  
T Nagata
Keyword(s):  
Epithelial Cells ◽  
Dna Synthesis ◽  
Goblet Cells ◽  
Autoradiographic Study ◽  
Lower Half ◽  
Labeling Index ◽  
Absorptive Cells ◽  
Significant Difference

The localization and activity of DNA synthesis of mouse cecal epithelia from prenatal Day 19 embryos to 12-month adults were investigated by light microscopic autoradiography after [3H]-thymidine injection in vivo. The cecal epithelial labeled cells with [3H]-thymidine were located at the lower half of the crypts, and the labeling index (LI) changed with the development of the cecum due to aging. A peak in the LI of absorptive cells was found at embryonic Day 19, but it decreased strikingly at the first postnatal day and then kept an almost constant value until 12 months. The LI of the goblet cells showed the highest value at fetal Day 19, decreased gradually with aging from the first postnatal day, and completely disappeared from 1 month onwards. The basal granulated cells were located only in the base of crypts, and the significant difference of LI was not found from embryonic Day 19 through 12-month adult. The results obtained in this study suggest that there are some differences of DNA synthesis among different kinds of cecal epithelial cells from embryonic stages to postnatal stages as a result of aging.

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