Monoclonal antibodies to group a streptococal polysaccharide, cross-reacting with mammalian connective tissue

1988 ◽  
Vol 105 (6) ◽  
pp. 850-853
Author(s):  
E. I. Drobyshevskaya ◽  
E. Yu. Pyt'eva ◽  
I. M. Lyampert ◽  
N. A. Borodiyuk ◽  
V. Yu. Sanina ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Connective Tissue ◽  
Group A

scholarly journals A Survey of G6 and G10 Serotypes of Group a Bovine Rotaviruses from Diarrheic Beef and Dairy Calves using Monoclonal Antibodies in ELISA

1994 ◽  
Vol 6 (2) ◽  
pp. 175-181 ◽  
Author(s):  
A. Lucchelli ◽  
S. Y. Kang ◽  
M. K. Jayasekera ◽  
A. V. Parwani ◽  
D. H. Zeman ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
South Dakota ◽  
Dairy Herd ◽  
Dairy Calves ◽  
Enzyme Linked Immunosorbent Assay ◽  
Positive Stool ◽  
Field Samples ◽  
Group A Rotaviruses ◽  
Beef Herds ◽  
Group A

Group A bovine rotaviruses (BRV) have been identified worldwide as a major cause of diarrhea in the young of many species, including humans. Group A rotaviruses are classified into serotypes on the basis of the outer capsid proteins, VP7 (G types) and VP4 (P types). To date, there are 14 G types of group A rotaviruses, with G1, G6, G8, and G10 described for BRV isolates. In this study, G6- and G lo-specific monoclonal antibodies (MAbs) were used in an enzyme-linked immunosorbent assay (ELISA) for the G typing of BRV-positive stool samples from diarrheic beef and dairy calves from South Dakota, Ohio, Michigan, Nebraska, and Washington, USA, and Ontario, Canada. ELISA plates were coated using a broadly reactive VP7 MAb (Common 60) or with G6- or G10-specific MAbs. BRV-positive fecal samples were diluted and added to duplicate wells, followed by the addition of polyclonal guinea pig anti-group A rotavirus serum as the secondary antibody. Several reference G6 and G10 BRV strains as well as other G types previously reported in cattle (G1, G2, G3, G8) and BRV-negative samples were included as G type specificity and negative controls. From a total of 308 field samples analyzed, 79% (244/308) tested positive by the broadly reactive VP7 MAb; of these, 54% (131/244) were G6 positive, 14% (35/244) were G10 positive, 4% (9/244) were both G6 and G10 positive, and 28% (69/244) were G6 and G10 negative. The negative samples may represent additional or undefined serotypes. The 89 samples from South Dakota were further subdivided into samples from beef ( n = 43) or dairy ( n = 46) herds. G6 was more prevalent in beef herd samples (67%) than in dairy herd samples (47.5%). In addition, dairy herds had higher percentages of G10-positive samples (17.5%) G6-G10 double positives (10%), and untypable samples (25%) than did beef herds, in which the prevalence of G10 positive samples was 5.5%, G6-G10 double positives was 5.5%, and untypable samples was 22%. Application of the serotype ELISA for the analysis of additional BRV samples will provide further epidemiologic data on the distribution of BRV serotypes in beef or dairy cattle, an important consideration for the development of improved BRV vaccines.

scholarly journals Improved procedure for the construction of neoglycolipids having antigenic and lectin-binding activities, from reducing oligosaccharides

1988 ◽  
Vol 256 (2) ◽  
pp. 661-664 ◽  
Author(s):  
M S Stoll ◽  
T Mizuochi ◽  
R A Childs ◽  
T Feizi
Keyword(s):  
Monoclonal Antibodies ◽  
Blood Group ◽  
Concanavalin A ◽  
Lectin Binding ◽  
Blood Group A ◽  
Naturally Occurring ◽  
Group A ◽  
High Mannose ◽  
B Blood Group

Conditions have been established for the rapid and efficient conjugation of reducing oligosaccharides (di- to deca-saccharides) to dipalmitoyl phosphatidylethanolamine. The resulting neoglycolipids derived from several naturally occurring oligosaccharides and a series of N-linked high-mannose-type oligosaccharides released by hydrazinolysis from RNAase B showed specific and potent reactivities, as appropriate, with monoclonal antibodies to blood group Lewis(b), blood group A or a stage-specific embryonic (SSEA-1) antigen, or the lectin concanavalin A.

Monoclonal Antibodies Against Membrane Glycolipids of an Anti-Streptococcal Group a b-Cell Hybridoma

1982 ◽  
pp. 797-800
Author(s):  
R.K. BALL ◽  
H. HERBST ◽  
C.A. SCHÖNENBERGER ◽  
G. ROSENFELDER ◽  
D.G. BRAUN
Keyword(s):  
Monoclonal Antibodies ◽  
B Cell ◽  
Group A

scholarly journals Three mouse monoclonal antibodies to human differentiation antigens: reactivity with two mucin-like antigens and with connective tissue fibers.

1985 ◽  
Vol 33 (11) ◽  
pp. 1095-1102 ◽  
Author(s):  
M J Mattes ◽  
K Look ◽  
J L Lewis ◽  
L J Old ◽  
K O Lloyd
Keyword(s):  
Monoclonal Antibodies ◽  
Epithelial Cells ◽  
Connective Tissue ◽  
Cell Lines ◽  
Frozen Sections ◽  
Cultured Fibroblasts ◽  
Differentiation Antigens ◽  
Normal Tissues ◽  
Carcinoma Cell Lines ◽  
Histologic Types

Three human differentiation antigens (MU78, MT334, and MQ49) have been defined by mouse monoclonal antibodies developed from mice immunized with ovarian carcinoma cell lines. Their distribution was determined on 148 cultured cell lines of various histologic types and on frozen sections of 16 normal tissues. MU78 was found in fibrillar structures in soft connective tissue with a distribution resembling that of elastin fibers; however, elastin fibers in elastic cartilage and in the aorta were nonreactive. MU78 was detected in cultured carcinoma cells of various histologic types, where it had a nonfibrillar, cytoplasmic distribution, but was not detected in normal epithelial cells in frozen sections. Cultured fibroblasts, astrocytomas, melanomas, and lymphomas did not contain MU78. In cell lines, MU78 appears to be a protein of 2000-5000 daltons. The other two antigens, MT334 and MQ49, are both mucin-like molecules, and the determinants are probably carbohydrate in nature. Of the normal tissues examined, MT334 was detected only in goblet cells of the colon, though it was present in a variety of carcinomas in culture. It was detected as both a cytoplasmic and secreted component. MQ49 was detected in various secretory epithelial cells, in Hassall's corpuscles in the thymus, and in cultured carcinomas of various histologic types. It was found on the cell surface as well as in the cytoplasm and is present on a glycolipid as well as on a sulfated mucin. These results, and results of other recent studies, demonstrate the importance of mucin-like molecules as antigens in epithelial cells and secretions.

Specificity of monoclonal antibodies obtained by immunization of mice with trypsin-treated group a streptococcus culture

1994 ◽  
Vol 118 (5) ◽  
pp. 1189-1192
Author(s):  
T. A. Danilova ◽  
T. K. Asoskova ◽  
N. A. Borodiyuk ◽  
L. V. Beletskaya ◽  
V. G. Nesterenko
Keyword(s):  
Monoclonal Antibodies ◽  
Group A Streptococcus ◽  
Treated Group ◽  
Group A
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