Regulation of Natural killer activity of lymphocytes from normal subjects and patients with chronic lymphatic leukemia by interaction between T and non-T cells

1987 ◽  
Vol 103 (3) ◽  
pp. 359-361
Author(s):  
N. A. Khonina ◽  
G. Z. Shubinskii ◽  
V. P. Lozovoi
Keyword(s):  
T Cells ◽  
Natural Killer ◽  
Natural Killer Activity ◽  
Normal Subjects ◽  
Chronic Lymphatic Leukemia ◽  
Killer Activity ◽  
Lymphatic Leukemia

Effect of Indomethacin and Thymostimulin on Natural Killer Activity of Thalassemic Patients and Normal Subjects

1992 ◽  
Vol 5 (1) ◽  
pp. 23-30
Author(s):  
Maria Aranzazu Goicoa ◽  
I.J. Ballart ◽  
M. Fernanda Palacios ◽  
Susana A. De Miani ◽  
Maria E. Estevez ◽  
...  
Keyword(s):  
T Cells ◽  
Natural Killer ◽  
Natural Killer Activity ◽  
Normal Subjects ◽  
Thalassemia Major ◽  
Con A ◽  
Killer Activity ◽  
Natural Killer Cytotoxicity ◽  
Normal Controls

To evaluate whether indomethacin (IM) and thymostimulin (TP-1) could revert the alterations of natural killer cytotoxicity (NKC) observed in patients and carriers of Thalassemia Major (TM), 14 TM patients, 10 TM carriers, 16 normal controls, and 4 polytransfused patients were studied. In vitro, IM induced an increase in NKC of normal controls and TM carriers (Δ CI: + 5.98±2.49 and + 9.77±6.82 respectively), but not in TM patients (-0.32±1.45) or other polytransfused recipients. The addition of IM to Concanavalin A (Con A) also resulted in an increase of NKC in normal controls and TM carriers, similar to that induced by each substance separately in normal controls, or by IM alone in TM carriers. Similarly, TP-1 (50 μg/ml) induced increases of NKC in normal subjects (+ 4.66±3.62), but not in TM patients (-1.1±2.43). The impairment in NKC observed in TM and polytransfused patients, and the absence of response to Con A in both, TM patients and carriers, do not seem to be mediated by an excessive production of prostaglandins. The lack of response not only to Con A, but also to TP-1 could indicate a quantitative or qualitative defect in T cells, that affects NKC regulation, or a deficient response of NK cells to modulators produced by T lymphocytes.

scholarly journals The OX-44 molecule couples to signaling pathways and is associated with CD2 on rat T lymphocytes and a natural killer cell line.

1992 ◽  
Vol 175 (2) ◽  
pp. 527-536 ◽  
Author(s):  
G M Bell ◽  
W E Seaman ◽  
E C Niemi ◽  
J B Imboden
Keyword(s):  
T Cells ◽  
Signaling Pathways ◽  
Natural Killer ◽  
Peptide Mapping ◽  
Inositol Phosphates ◽  
Killer Cell ◽  
Natural Killer Activity ◽  
Free Calcium ◽  
Killer Activity ◽  
Peripheral Leukocytes

The MRC OX-44 molecule, which is expressed on all peripheral leukocytes, identifies the subset of thymocytes capable of proliferating in response to alloantigens and lectins (Paterson, D.J., J.R. Green, W.A. Jefferies, M. Puklavec, and A.F. Williams. 1987. J. Exp. Med. 165:1). When we isolated monoclonal antibodies (mAbs) on the basis of their ability to activate the phosphatidylinositol signaling pathway in RNK-16 cells (a rat leukemia line with natural killer activity), three of the resulting mAbs recognized the OX-44 molecule. Addition of these mAbs to RNK-16 elicits protein tyrosine phosphorylation, generates inositol phosphates, and increases the concentration of cytoplasmic free calcium. These responses require the addition of intact mAb and are not observed with F(ab')2 fragments. One of these mAbs (7D2) is mitogenic for freshly isolated rat splenic T cells and synergizes with a mAb to the T cell antigen receptor in this activation. A 50-60-kD glycoprotein coprecipitates with the OX-44 molecule from RNK-16 cells and rat splenic T cells. Peptide mapping and reprecipitation studies indicate that the coprecipitating molecule is CD2. Thus, the OX-44 molecule can couple to multiple signaling pathways and associates with CD2 on both RNK-16 and rat T cells.

scholarly journals Decreased CD45RA T cells in B-cell chronic lymphatic leukemia patients: correlation with disease stage

Blood ◽  
1991 ◽  
Vol 78 (6) ◽  
pp. 1569-1573 ◽  
Author(s):  
S Peller ◽  
S Kaufman
Keyword(s):  
T Cells ◽  
B Cell ◽  
Suppressor Cells ◽  
Normal Subjects ◽  
T Cell Subsets ◽  
Disease Stage ◽  
Chronic Lymphatic Leukemia ◽  
Early Stages ◽  
Lymphatic Leukemia ◽  
Advanced Stages

T-cell subsets CD4, CD8 and suppressor-inducers (CD45RA) were determined in 20 patients with B-cell chronic lymphatic leukemia (B- CLL). The proportion of CD4 and CD45RA was decreased when compared with T cells from normal subjects. CD8 was markedly increased. The activity of concanavalin A-induced suppressor cells was not significantly different from that of normal controls and was negatively correlated to the percentage of CD4 of B-CLL patients. The selective loss of CD45RA cells was more prominent in patients in advanced Rai stages of the disease (III to IV) than in early stages (0 to II). Six patients of the advanced stages group suffered from autoimmune hemolytic anemia, whereas no patient in the early stages of disease showed an autoimmune phenomenon. Our results may indicate a mechanism of autoimmunity in B- CLL similar to that of patients with autoimmune diseases.

Increased number of Leu-2-bearing non-T cells with natural killer activity in chimpanzees

1986 ◽  
Vol 97 (1) ◽  
pp. 164-172 ◽  
Author(s):  
Thomas M. Folks ◽  
Thomas M. Chused ◽  
Diane Portnoy ◽  
Linette Edison ◽  
William Leiserson ◽  
...  
Keyword(s):  
T Cells ◽  
Natural Killer ◽  
Natural Killer Activity ◽  
Killer Activity

scholarly journals Decreased CD45RA T cells in B-cell chronic lymphatic leukemia patients: correlation with disease stage

Blood ◽  
1991 ◽  
Vol 78 (6) ◽  
pp. 1569-1573 ◽  
Author(s):  
S Peller ◽  
S Kaufman
Keyword(s):  
T Cells ◽  
B Cell ◽  
Suppressor Cells ◽  
Normal Subjects ◽  
T Cell Subsets ◽  
Disease Stage ◽  
Chronic Lymphatic Leukemia ◽  
Early Stages ◽  
Lymphatic Leukemia ◽  
Advanced Stages

Abstract T-cell subsets CD4, CD8 and suppressor-inducers (CD45RA) were determined in 20 patients with B-cell chronic lymphatic leukemia (B- CLL). The proportion of CD4 and CD45RA was decreased when compared with T cells from normal subjects. CD8 was markedly increased. The activity of concanavalin A-induced suppressor cells was not significantly different from that of normal controls and was negatively correlated to the percentage of CD4 of B-CLL patients. The selective loss of CD45RA cells was more prominent in patients in advanced Rai stages of the disease (III to IV) than in early stages (0 to II). Six patients of the advanced stages group suffered from autoimmune hemolytic anemia, whereas no patient in the early stages of disease showed an autoimmune phenomenon. Our results may indicate a mechanism of autoimmunity in B- CLL similar to that of patients with autoimmune diseases.

scholarly journals Kinetics of in vitro natural killer activity against K562 cells as detected by flow cytometry

Cytometry ◽  
1998 ◽  
Vol 32 (4) ◽  
pp. 280-285 ◽  
Author(s):  
Loris Zamai ◽  
Adriana R. Mariani ◽  
Giorgio Zauli ◽  
Luigi Rodella ◽  
Rita Rezzani ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Natural Killer ◽  
Natural Killer Activity ◽  
K562 Cells ◽  
Killer Activity ◽  
Kinetics Of
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