Effect of bronchial mucus of bronchial asthma patients and chronic bronchitis on ciliary activity of ciliated epithelial cells

1990 ◽  
Vol 110 (3) ◽  
pp. 1282-1285
Author(s):  
N. A. Shanina ◽  
I. I. Dykhanov
Keyword(s):  
Epithelial Cells ◽  
Chronic Bronchitis ◽  
Bronchial Asthma ◽  
Ciliary Activity ◽  
Asthma Patients ◽  
Bronchial Mucus ◽  
Ciliated Epithelial Cells

Effects of α-Toxin ofStaphylococcus aureuson the Ciliary Activity and Ultrastructure of Human Nasal Ciliated Epithelial Cells

1999 ◽  
Vol 109 (12) ◽  
pp. 2021-2024 ◽  
Author(s):  
Yeong-Seok Yun ◽  
Yang-Gi Min ◽  
Chae-Seo Rhee ◽  
In-Ho Jung ◽  
Young Yull Koh ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Ciliary Activity ◽  
Ciliated Epithelial Cells

Pathogenesis of infectious bovine rhinotracheitis (IBR) virus infection in bovine fetal tracheal organ cultures

1974 ◽  
Vol 20 (6) ◽  
pp. 839-845 ◽  
Author(s):  
Wha-Kiam Chia ◽  
M. Savan
Keyword(s):  
Epithelial Cells ◽  
Cytopathic Effect ◽  
Cell Types ◽  
Ciliary Activity ◽  
Epithelial Layer ◽  
Organ Cultures ◽  
Epithelial Surface ◽  
Antibody Preparations ◽  
Ciliated Epithelial Cells ◽  
Hematoxylin Eosin

Bovine fetal tracheal organ cultures were infected with a field strain of IBR virus and the effect of the virus was assessed by observing ciliary activity and cytopathic changes in unstained, hematoxylin-eosin-stained, and fluorescent-labeled antibody preparations of the culture system. The virus was quantitated in bovine fetal kidney cell cultures. The IBR virus was first detected in infected organ cultures at 8–12 h postinoculation and persisted for at least 23 days. A decrease in ciliary activity was not observed until 48 h postinoculation, at the same time that the first signs of cytopathic effect was seen in the unstained cultures. The viral infection first appeared as discrete foci of rounded cells on the epithelial surface, but then spread from cell to cell beneath the ciliated epithelial cells leading to early degeneration of the non-ciliated epithelial cells and microvesiculation which eventually resulted in a massive sloughing of the whole epithelial layer. The virus was not limited to the epithelial layer but infected a variety of cell types among the submucosal and connective tissue components of the organ cultures.

MYCOPLASMA AND CHLAMYDIA AS ETHIOLOGICAL FACTORS OF BRONCHIAL ASTHMA IN TERMS OF ETHNOGENESIS

2013 ◽  
Vol 68 (7) ◽  
pp. 57-60
Author(s):  
O. A. Sharavii ◽  
S. V. Smirnova
Keyword(s):  
Immune Response ◽  
Chlamydia Trachomatis ◽  
Blood Serum ◽  
Bronchial Asthma ◽  
Mycoplasma Hominis ◽  
Acute Stage ◽  
Control Group ◽  
Clinical Markers ◽  
Chlamydophila Psittaci ◽  
Asthma Patients

 Aim. The study of the prevalence and clinical peculiarities of Mycoplasmosis and Chlamydiosis in patients with different pathogenic forms of bronchial asthma (BA) taking into account ethnicity of a patient. Subjects and Methods. The research covered 239 subjects – both the Europeoids and the Mongoloids in the city of Krasnoyarsk and the town of Kyzyl, all of them being BA patients of different stages, including acute stage and practically healthy. We had determined antigens Mycoplasma pneumoniae, Mycoplasma hominis, Chlamydophila pneumoniae, Chlamydophila psittaci and Chlamydia trachomatis in smears of mucosa of pharynx and antibodies to these antigens in peripheral blood serum. Results.  We found high frequency of Mycoplasmosis and Chlamydiosis in the inhabitants of Eastern Siberia, BA patients with different pathogenic forms as compared to control group. We had determined ethnic peculiarities of specific immune response: IgM to М. pneumoniae was revealed in the Europoids more frequently than in the Mongoloids, but IgM to С. pneumoniae and to C. trachomatis, C. trachomatis antigens had been revealed more often in the Mongoloids than in the Europoids. We accepted as clinical equivalents of Mycoplasmosis and Chlamydiosis diagnostics the following signs: temperature around 37C (subfebrile temperature), non-intensive but stable coughing with scanty mucous and muco-purulent sputum, dyspnea of mixed character. Conclusions. Mycoplasma and Chlamydia are meaningful etiologic factors of bronchial asthma. We have found the peculiarities of immune response depending on ethnicity of a patient (ethnic belonging). Clinical markers of Mycoplasmosis and Chlamydiosis should be taken into account in bronchial asthma in order to provide diagnostics timely as well as eradication of infection agents. Because of insufficient knowledge of problem of bronchial asthma related to contamination with Мycoplasma and Chlamydia we put the goal to study the frequency of Mycoplasmosis and Chlamydiosis occurrence in bronchial asthma patients and determine the characteristics clinical course of diseases. We defined antigens Мycoplasma pneumoniae, Мycoplasma hominis, Chlamydophila pneumoniaе, Chlamydophila psittaci, Chlamydia trachomatis in smears of oropharynx mucosa and antibodies to them in blood serum. 

scholarly journals Lung macrophages drive mucus production and steroid-resistant inflammation in chronic bronchitis

2021 ◽  
Vol 22 (1) ◽  
Author(s):  
Kristina Andelid ◽  
Karolina Öst ◽  
Anders Andersson ◽  
Esha Mohamed ◽  
Zala Jevnikar ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Chronic Bronchitis ◽  
Cross Talk ◽  
Alveolar Macrophages ◽  
Ex Vivo ◽  
Bronchial Epithelial Cells ◽  
Steroid Resistance ◽  
Bronchial Epithelial ◽  
Steroid Resistant ◽  
Copd Patients

Abstract Background Patients with chronic obstructive pulmonary disease (COPD) frequently suffer from chronic bronchitis (CB) and display steroid-resistant inflammation with increased sputum neutrophils and macrophages. Recently, a causal link between mucus hyper-concentration and disease progression of CB has been suggested. Methods In this study, we have evaluated the steroid sensitivity of purified, patient-derived sputum and alveolar macrophages and used a novel mechanistic cross-talk assay to examine how macrophages and bronchial epithelial cells cross-talk to regulate MUC5B production. Results We demonstrate that sputum plug macrophages isolated from COPD patients with chronic bronchitis (COPD/CB) are chronically activated and only partially respond to ex vivo corticosteroid treatment compared to alveolar macrophages isolated from lung resections. Further, we show that pseudo-stratified bronchial epithelial cells grown in air–liquid-interface are inert to direct bacterial lipopolysaccharide stimulation and that macrophages are able to relay this signal and activate the CREB/AP-1 transcription factor complex and subsequent MUC5B expression in epithelial cells through a soluble mediator. Using recombinant protein and neutralizing antibodies, we identified a key role for TNFα in this cross-talk. Conclusions For the first time, we describe ex vivo pharmacology in purified human sputum macrophages isolated from chronic bronchitis COPD patients and identify a possible basis for the steroid resistance frequently seen in this population. Our data pinpoint a critical role for chronically activated sputum macrophages in perpetuating TNFα-dependent signals driving mucus hyper-production. Targeting the chronically activated mucus plug macrophage phenotype and interfering with aberrant macrophage-epithelial cross-talk may provide a novel strategy to resolve chronic inflammatory lung disease.

Comparison between Compliance of Fluticasone Propionate Diskhaler and of Fluticasone Propionate Diskus in Adult Bronchial Asthma Patients

Respiration ◽  
2006 ◽  
Vol 73 (5) ◽  
pp. 680-684 ◽  
Author(s):  
Takashi Hasegawa ◽  
Eiichi Suzuki ◽  
Katsuya Fujimori ◽  
Takuro Sakagami ◽  
Shinichi Toyabe ◽  
...  
Keyword(s):  
Fluticasone Propionate ◽  
Bronchial Asthma ◽  
Asthma Patients

Differential effects of UTP, ATP, and adenosine on ciliary activity of human nasal epithelial cells

2001 ◽  
Vol 280 (6) ◽  
pp. C1485-C1497 ◽  
Author(s):  
Diane M. Morse ◽  
Jennifer L. Smullen ◽  
C. William Davis
Keyword(s):  
Epithelial Cells ◽  
Beat Frequency ◽  
Ciliary Beat Frequency ◽  
Ciliary Activity ◽  
Maximal Effect ◽  
Ciliary Beating ◽  
Mediated Effects ◽  
Human Nasal Epithelium ◽  
Human Nasal Epithelial Cells ◽  
Sustained Responses

The purinergic regulation of ciliary activity was studied using small, continuously superfused explants of human nasal epithelium. The P2Y2 purinoceptor (P2Y2-R) was identified as the major purinoceptor regulating ciliary beat frequency (CBF); UTP (EC50 = 4.7 μM), ATP, and adenosine-5′- O-(3-thiotriphosphate) elicited similar maximal responses, approximately twofold over baseline. ATP, however, elicited a post-peak sustained plateau in CBF (1.83 ± 0.1-fold), whereas the post-peak CBF response to UTP declined over 15 min to a low-level plateau (1.36 ± 0.16-fold). UDP also stimulated ciliary beating, probably via P2Y6-R, with a maximal effect approximately one-half that elicited by P2Y2-R stimulation. Not indicated were P2Y1-R-, P2Y4-R-, or P2Y11-R-mediated effects. A2B-receptor agonists elicited sustained responses in CBF approximately equal to those from UTP/ATP [5′-( N-ethylcarboxamido)adenosine, EC50 = 0.09 μM; adenosine, EC50 = 0.7 μM]. Surprisingly, ADP elicited a sustained stimulation in CBF. The ADP effect and the post-peak sustained portion of the ATP response in CBF were inhibited by the A2-R antagonist 8-( p-sulfophenyl)theophylline. Hence, ATP affects ciliary activity through P2Y2-R and, after an apparent ectohydrolysis to adenosine, through A2BAR.

Shedding of Infected Ciliated Epithelial Cells in Rhinovirus Colds

1982 ◽  
Vol 145 (6) ◽  
pp. 849-853 ◽  
Author(s):  
R. B. Turner ◽  
J. O. Hendley ◽  
J. M. Gwaltney
Keyword(s):  
Epithelial Cells ◽  
Ciliated Epithelial Cells
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