A study of bovine blood serum γ-globulin and of lymph gland tissue proteins with corresponding isoelectric points by the peptide map method

1971 ◽  
Vol 72 (6) ◽  
pp. 1395-1397
Author(s):  
I. F. Kiryukhin ◽  
S. N. Bagdasar'yan
Keyword(s):  
Blood Serum ◽  
Lymph Gland ◽  
Bovine Blood ◽  
Isoelectric Points ◽  
Gland Tissue ◽  
Peptide Map ◽  
Lymph Gland Tissue

Isolation of properdin preparation from bovine blood serum and study of its properties

1965 ◽  
Vol 60 (5) ◽  
pp. 1262-1265
Author(s):  
G. Ya. Rozenberg ◽  
V. Ya. Vissarionova ◽  
Yu. M. Mikhailova ◽  
N. D. Papush ◽  
V. Ya. Chernyak
Keyword(s):  
Blood Serum ◽  
Bovine Blood

Magnesium Determination in Bovine Blood Serum

1954 ◽  
Vol 26 (6) ◽  
pp. 1072-1073 ◽  
Author(s):  
Mitsuo. Okamoto ◽  
J. W. Thomas
Keyword(s):  
Blood Serum ◽  
Bovine Blood

scholarly journals Investigating the Use of Protein Saver Cards for Storage and Subsequent Detection of Bovine Anti-Brucella abortus Smooth Lipopolysaccharide Antibodies and Gamma Interferon

2013 ◽  
Vol 20 (11) ◽  
pp. 1669-1674 ◽  
Author(s):  
Lucy Duncombe ◽  
Nicola J. Commander ◽  
Sevil Erdenlig ◽  
John A. McGiven ◽  
Judy Stack
Keyword(s):  
Blood Serum ◽  
Brucella Abortus ◽  
Room Temperature ◽  
Plasma Samples ◽  
Bovine Blood ◽  
Bovine Brucellosis ◽  
Serum Samples ◽  
Content Type ◽  
Antibody Titers ◽  
Ifn Γ

ABSTRACTBrucella abortus, a smooth strain of the genusBrucella, is the causative agent of bovine brucellosis. To support the ongoing development of diagnostic tests for bovine brucellosis, the use of Protein Saver cards (Whatman) for bovine blood serum and plasma sample collection has been evaluated. These cards offer significant logistical and safety alternatives to transporting and storing liquid samples and may aid in diagnostic programs and validation studies. To evaluate the utility of these cards, 204 bovine blood serum samples fromBrucella-infected and noninfected animals were stored on and eluted from the Protein Saver cards. Anti-Brucellasmooth lipopolysaccharide (sLPS) antibody titers for the serum eluates were compared to those of the unprocessed original serum samples by indirect enzyme-linked immunosorbent assay (ELISA). The results showed a highly significant correlation between titers from the serum eluates and the unprocessed sera. Therefore, under these circumstances, serum eluates and unprocessed serum samples may be used interchangeably. Blood plasma from 113 mitogen-stimulated whole-blood samples was added to and eluted from the Protein Saver cards. The gamma interferon (IFN-γ) titers in the plasma eluates were compared to those of the unprocessed plasma samples obtained by IFN-γ ELISA. The results showed a significant correlation between the plasma eluates and the unprocessed plasma samples. To derive a signal in the plasma eluate, it was necessary to develop a novel and highly sensitive ELISA for the detection of IFN-γ. The serum samples stored on cards at room temperature over a 10-day period showed little variation in antibody titers. However, the plasma eluates showed a progressive loss of IFN-γ recovery over 10 days when stored at room temperature.

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