LH, Prolactin, Estradiol and Progesterone in Bovine Blood Serum during Early Pregnancy

1973 ◽  
Vol 36 (1) ◽  
pp. 51-56 ◽  
Author(s):  
R. P. Wettemann ◽  
H. D. Hafs
Keyword(s):  
Blood Serum ◽  
Early Pregnancy ◽  
Bovine Blood

Isolation of properdin preparation from bovine blood serum and study of its properties

1965 ◽  
Vol 60 (5) ◽  
pp. 1262-1265
Author(s):  
G. Ya. Rozenberg ◽  
V. Ya. Vissarionova ◽  
Yu. M. Mikhailova ◽  
N. D. Papush ◽  
V. Ya. Chernyak
Keyword(s):  
Blood Serum ◽  
Bovine Blood

Magnesium Determination in Bovine Blood Serum

1954 ◽  
Vol 26 (6) ◽  
pp. 1072-1073 ◽  
Author(s):  
Mitsuo. Okamoto ◽  
J. W. Thomas
Keyword(s):  
Blood Serum ◽  
Bovine Blood

scholarly journals Investigating the Use of Protein Saver Cards for Storage and Subsequent Detection of Bovine Anti-Brucella abortus Smooth Lipopolysaccharide Antibodies and Gamma Interferon

2013 ◽  
Vol 20 (11) ◽  
pp. 1669-1674 ◽  
Author(s):  
Lucy Duncombe ◽  
Nicola J. Commander ◽  
Sevil Erdenlig ◽  
John A. McGiven ◽  
Judy Stack
Keyword(s):  
Blood Serum ◽  
Brucella Abortus ◽  
Room Temperature ◽  
Plasma Samples ◽  
Bovine Blood ◽  
Bovine Brucellosis ◽  
Serum Samples ◽  
Content Type ◽  
Antibody Titers ◽  
Ifn Γ

ABSTRACTBrucella abortus, a smooth strain of the genusBrucella, is the causative agent of bovine brucellosis. To support the ongoing development of diagnostic tests for bovine brucellosis, the use of Protein Saver cards (Whatman) for bovine blood serum and plasma sample collection has been evaluated. These cards offer significant logistical and safety alternatives to transporting and storing liquid samples and may aid in diagnostic programs and validation studies. To evaluate the utility of these cards, 204 bovine blood serum samples fromBrucella-infected and noninfected animals were stored on and eluted from the Protein Saver cards. Anti-Brucellasmooth lipopolysaccharide (sLPS) antibody titers for the serum eluates were compared to those of the unprocessed original serum samples by indirect enzyme-linked immunosorbent assay (ELISA). The results showed a highly significant correlation between titers from the serum eluates and the unprocessed sera. Therefore, under these circumstances, serum eluates and unprocessed serum samples may be used interchangeably. Blood plasma from 113 mitogen-stimulated whole-blood samples was added to and eluted from the Protein Saver cards. The gamma interferon (IFN-γ) titers in the plasma eluates were compared to those of the unprocessed plasma samples obtained by IFN-γ ELISA. The results showed a significant correlation between the plasma eluates and the unprocessed plasma samples. To derive a signal in the plasma eluate, it was necessary to develop a novel and highly sensitive ELISA for the detection of IFN-γ. The serum samples stored on cards at room temperature over a 10-day period showed little variation in antibody titers. However, the plasma eluates showed a progressive loss of IFN-γ recovery over 10 days when stored at room temperature.

Efficacy of a commercial test kit to determine early pregnancy in cows using whole blood and blood serum

2020 ◽  
Vol 53 (1) ◽  
Author(s):  
Ozan Karakuş ◽  
Cihan Kaçar ◽  
Mushap Kuru ◽  
Semra Kaya ◽  
Murat Can Demir ◽  
...  
Keyword(s):  
Blood Serum ◽  
Early Pregnancy ◽  
Whole Blood ◽  
Commercial Test ◽  
Test Kit

Interrelationship of some hormonesbelonging to HPG and energy axes inhorses during various reproductive states

2015 ◽  
Vol 15 (2) ◽  
pp. 10-15
Author(s):  
D Batsaikhan ◽  
B Bayar-Enkh ◽  
B Tuvshin
Keyword(s):  
Insulin Resistance ◽  
Blood Serum ◽  
Early Pregnancy ◽  
Metabolic Disorder ◽  
Endocrine System ◽  
Late Pregnancy ◽  
Reproductive Activity ◽  
Inhibin B ◽  
Metabolic Hormones ◽  
Activity Concentrations

In order to reveal interrelationships of hormones during pregnancy and estrus in animals, some reproductive and metabolic hormones including FSH, inhibin B, prolactin and insulin were measured in blood serum of Mongolian horses with respective immunoassays. Concentrations of blood serum FSH, inhibin B and prolactin in mares during their estrus, early, mid and late pregnancy periods were compared. The comparisons demonstrate concentrations of FSH and inhibin B were inversely proportional especially during estrous cycle of mares. As well, these hormones were also measured in blood serum of stallions with the purpose of assessing their reproductive activity. Concentrations of both hormones were lower in blood serum of stallions, which have harems of less conceived mares or it means higher inhibin concentration results in less reproductive activity. Prolactin was minimal during estrus and early pregnancy, while it increases during late pregnancy, but there was no correlations with above two hormones. Measurements of insulin was helpful to learn whether the animal is suffering from metabolic disorder such as insulin resistance, because last half of pregnancy of mares coincides with the period of fattening of pastured animals. However, main outcome of the present study is that all above hormones might be acting in closer relationships during various reproductive states, despite they belong to different axes of endocrine system, including hypothalamic-pituitary-gonadal (FSH and inhibin)and energy (insulin and prolactin) axes.Mongolian Journal of Agricultural Sciences Vol.15(2) 2015; 10-15

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