Effect of regeneration of the spleen and bone marrow on number of hematopoietic colonies in the mouse spleen

1976 ◽  
Vol 82 (5) ◽  
pp. 1720-1722
Author(s):  
G. K. Sukhova ◽  
T. R. Podrabinek ◽  
G. V. Kharlova
Keyword(s):  
Bone Marrow ◽  
Mouse Spleen ◽  
Regeneration Of The Spleen

Biopterin Synthesis in Mouse Spleen during Bone Marrow Transplantation Correlates with Unimpaired Hemopoietic Engraftment

1987 ◽  
Vol 42 (4) ◽  
pp. 461-464 ◽  
Author(s):  
Irmgard Ziegler ◽  
Stefan Thierfelder
Keyword(s):  
Monoclonal Antibody ◽  
Bone Marrow ◽  
Donor Cell ◽  
Mouse Spleen ◽  
Graft Versus Host Reaction ◽  
Allogeneic Bone ◽  
Bone Marrow Transplants ◽  
Host Reaction ◽  
Graft Versus Host ◽  
Biopterin Synthesis

Abstract Normal engraftment of bone marrow transplants depends on histocompatibility between donor and recipient. In this case reconstitution of hemopoiesis and lymphopoiesis in the mouse spleen is correlated with transient biopterin synthesis in these cells. Allogeneic bone marrow progeny cells undergo donor cell proliferation in the spleen prior to the incidence of graft-versus-host reaction. These cells are not committed to biopterin synthesis. Thy-1 monoclonal antibody fully repairs engraftment of allogeneic transplants together with biopterin synthesis.

Cytogenetic Effects of the Insecticide Methamidophos in Mouse Bone Marrow and Cultured Mouse Spleen Cells*

1987 ◽  
Vol 42 (1-2) ◽  
pp. 21-30 ◽  
Author(s):  
Soheir M. Amer ◽  
Magdy A. Sayed
Keyword(s):  
Bone Marrow ◽  
Chromosomal Aberrations ◽  
Mouse Spleen ◽  
Mouse Bone Marrow ◽  
Spleen Cells ◽  
Mutagenic Potential ◽  
Cells In Culture ◽  
Polychromatic Erythrocytes

Abstract The cytogenetic effect of the insecticide methamidophos (0,S-dimethylphosphoroamidothiolate) was studied in mouse bone marrow and mouse spleen cells in culture. In vivo the ability of methamidophos to induce micronuclei and sisterchromatid exchange in mouse bone marrow was investigated. In vitro mouse spleen cells in culture were used to assess the ability of the insecticide to induce chromosomal aberrations and sister chromatid exchange. Three different routes of application for the pure insecticide were tested so as to cover the different possibilities for human exposure to the insecticide. Intraperitoneal, oral and dermal treatment with metham idophos caused toxicity to marrow as indicated bv a significant increase in the percentage of polychromatic erythrocytes (PEs) over that of the control. Methamidophos showed mutagenic potential as evidenced by a positive response in the micronucleus and chromosomal aberrations assays. Thus, single and multiple i.p. injections at 6 and 4.5 mg methamidophos/kg body w t., oral administration of the insecticide for 14 consecutive days at a dietary level of 100 ppm and multiple dermal treatments (total 4) with 24 mg/kg body wt. induced a statistically significant increase in the frequency of PEs with micronuclei in mouse bone marrow. Moreover, the tested concentrations of m etham idophos as low as 0.25 ng/ml induced a high percentage of metaphases with chromosomal aberrations in cultured mouse spleen cells. Methamidophos is a weak inducer of SCEs in mouse bone marrow and cultured mouse spleen cells.

scholarly journals Phagocytized Plasma Cells in Mouse Spleen Observed by Light and Electron Microscopy

Blood ◽  
1964 ◽  
Vol 24 (4) ◽  
pp. 432-442 ◽  
Author(s):  
D. C. SWARTZENDRUBER
Keyword(s):  
Electron Microscopy ◽  
Bone Marrow ◽  
Microscopic Study ◽  
Plasma Cells ◽  
Light And Electron Microscopy ◽  
Mouse Spleen ◽  
White Pulp ◽  
Electron Microscopic ◽  
Single Intravenous Injection ◽  
Rat Bone

Abstract A correlative light and electron microscopic study is reported of pyknotic, phagocytized plasma cells in the mouse spleen following a single intravenous injection of sheep erythrocytes or rat bone marrow. Phagocytized plasma cells were observed in both red and white pulp between 3 and 7 days after antigen injection. A few mature plasma cells and plasma cells with Russel bodies were found 10 days after stimulation with no evidence of recognizable phagocytized plasma cells. Phagocytosis clearly plays some role in the removal of plasma cells from the spleen under the conditions employed in this study, although the possibility that significant numbers of plasmacytes migrate out of the spleen is considered. A correlation of phagocytized plasma cells with the log phase of antibody appearance in the circulation is indicated.

The Bone Marrow-Derived Stromal Cell Lines MC3T3-G2/PA6 and ST2 Support Osteoclast-Like Cell Differentiation in Cocultures with Mouse Spleen Cells

Endocrinology ◽  
1989 ◽  
Vol 125 (4) ◽  
pp. 1805-1813 ◽  
Author(s):  
NOBUYUKI UDAGAWA ◽  
NAOYUKI TAKAHASHI ◽  
TAKUHIKO AKATSU ◽  
TAKAHISA SASAKI ◽  
AKIRA YAMAGUCHI ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Cell Differentiation ◽  
Cell Lines ◽  
Stromal Cell ◽  
Mouse Spleen ◽  
Spleen Cells
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