Cytopathic changes in cells of chick embryo tissue culture affected by Newcastel's disease virus

1965 ◽  
Vol 59 (2) ◽  
pp. 187-190
Author(s):  
G. B. Yurovskaya
Keyword(s):  
Tissue Culture ◽  
Chick Embryo ◽  
Disease Virus ◽  
Embryo Tissue ◽  
In Cells

scholarly journals GROWTH OF THE FOWL CORYZA BODIES IN TISSUE CULTURE AND IN BLOOD AGAR

1939 ◽  
Vol 69 (2) ◽  
pp. 199-209 ◽  
Author(s):  
John B. Nelson
Keyword(s):  
Tissue Culture ◽  
Growth Factor ◽  
Chick Embryo ◽  
Agar Medium ◽  
Normal Growth ◽  
Cellular Component ◽  
Nutrient Media ◽  
Embryo Tissue ◽  
Cultural Requirements ◽  
Culture Supernatants

Evidence is presented that the growth capacity of chick embryo tissue for the fowl coryza bodies is conditioned by a diffusible cellular component which is essential for their multiplication. This growth factor is inactivated at pH 6, but withstands a temperature of 100°C. for 60 minutes. An amount sufficient to promote a normal growth of the specific bodies may be present in tissue culture supernatants long after its content in the tissue is exhausted. Postembryonic tissue (liver and spleen) contains a variable amount of growth factor and is not a satisfactory substitute for the chick embryo. Multiplication of recently isolated fowl coryza bodies is not demonstrable in nutrient media enriched with blood. Experiments with one strain, however, indicate that an adaptation to fluid blood in an agar medium may take place after many generations in tissue culture. The probable bacterial nature of the fowl coryza bodies is discussed on the basis of their cultural requirements.

scholarly journals Replication and attenuation of foot and mouth disease virus in chick embryo

2012 ◽  
Vol 9 (1) ◽  
pp. 31-35
Author(s):  
Baghdad Science Journal
Keyword(s):  
Tissue Culture ◽  
Chick Embryo ◽  
Guinea Pig ◽  
Disease Virus ◽  
Foot And Mouth Disease ◽  
Mouth Disease ◽  
Challenge Virus ◽  
Mouth Disease Virus ◽  
Attenuated Virus ◽  
Foot And Mouth

This study includes replication and attenuation of foot and mouth disease virus type O which isolated from infected calves. Many passages for the virus in chick-Embryo were established as a substitute method to the tissue culture which is highly caustic in contrast to the chick embryo. The virus passed ten consequent passages which lead to the reduce of the titer of the virus from 106.53 TCID50/ 0.1 ml in cattle testis tissue culture to 103 TCID50/ 0.1 ml. the pathogenecity of attenuated FMD virus were also studied in both chick-embryo and guinea pigs. Using agar gel diffusion test precipitation antibodies was detected in guinea pig serum after 14 and 21 days post exposure to the attenuated virus. The inoculated guinea pig group with the chick-embryo attenuated virus appear resistance to the challenge virus. The result suggested the efficacy of attenuation of foot and mouth disease virus by using chick-embryo system for immunization against this disease on the level of laboratory Animal.

‘Red Book’ Update

PEDIATRICS ◽  
1982 ◽  
Vol 70 (5) ◽  
pp. 819-822
Author(s):  
Vincent A. Fulginiti ◽  
Philip A. Brunell ◽  
James D. Cherry ◽  
Walton L. Ector ◽  
Anne A. Gershon ◽  
...  
Keyword(s):  
Tissue Culture ◽  
Chick Embryo ◽  
Measles Virus ◽  
The United States ◽  
Embryo Cell ◽  
Allergic Reactions ◽  
Chicken Feathers ◽  
Chick Embryo Cell ◽  
Anaphylactoid Reactions ◽  
Embryo Tissue

UPDATE The Red Book Committee met on May 10, 1982 and considered a number of issues, including: 1. The 1982 edition (19th) of the Red Book has been distributed beginning on June 4, 1982. We welcome comments and suggestions for the 20th edition. 2. IMPORTANT REVISION IN RED BOOK RECOMMENDATIONS: After the Committee meeting it came to our attention that three children who had anaphylactoid reactions to egg ingestion experienced immediate allergic reactions to chick-embryo-grown live measles virus vaccine; two had difficulty breathing and one had hypotension. Persons who are egg-allergic but do not have a history of anaphylactoid reactions appear to be at little or no risk from live measles virus (LMV) vaccine (See Morbidity Mortality Weekly Rep 31:217-231, May 7, 1982). Because previous experience indicated no adverse reactions in egg-allergic children given vaccine prepared in chick embryo tissue culture, the 1982 Red Book contains the following statement: The vaccine currently used in the United States is prepared in chick embryo tissue culture by inoculation with a further attenuated passage of the Edmonston B strain of measles virus. This preparation is virtually devoid of allergenic substances derived from the chick embryo cell cultures used for growth of the live vaccine viruses. However, there is a remote potential risk of hypersensitivity reactions in patients allergic to eggs, chicken, or chicken feathers; large scale use of the vaccine for more than a decade has resulted in only rare, isolated reports of minor allergic reactions. In a study in which children known to be allergic to eggs, chicken, or chicken feathers were vaccinated with a chick embryo cell culture-derived vaccine, no allergic reactions were observed.

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