Apoptosis in CHO cell batch cultures: examination by flow cytometry

1995 ◽  
Vol 17 (1) ◽  
pp. 1-11 ◽  
Author(s):  
Alison Moore ◽  
Christopher J. Donahue ◽  
Jeff Hooley ◽  
Diana L. Stocks ◽  
Kenneth D. Bauer ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Cho Cell ◽  
Batch Cultures

scholarly journals Application of Flow Cytometry to Segregated Kinetic Modeling Based on the Physiological States of Microorganisms

2007 ◽  
Vol 73 (12) ◽  
pp. 3993-4000 ◽  
Author(s):  
Covadonga Quir�s ◽  
M�nica Herrero ◽  
Luis A. Garc�a ◽  
Mario D�az
Keyword(s):  
Flow Cytometry ◽  
Culture Media ◽  
Glucose Consumption ◽  
Viable Cell ◽  
Counting Method ◽  
Batch Cultures ◽  
Viable Cells ◽  
The Status ◽  
Standard Culture

ABSTRACT Flow cytometry (FC) has been introduced to characterize and to assess the physiological states of microorganisms in conjunction with the classical plate-counting method. To show the applicability of the technique, in particular for the development of kinetic models, pure culture fermentation experiments were followed over time, using both prokaryotic (Lactobacillus hilgardii) and eukaryotic (Saccharomyces cerevisiae) microorganisms growing in standard culture media (MRS and YPD). The differences observed between the active and viable cells determined by FC and CFU, respectively, allowed us to determine that a large number of cells were in a viable but nonculturable (VBNC) state, which resulted in a subpopulation much larger than the damaged-cell (double-stained) subpopulation. Finally, the determination of the evolution of viable, the VBNC, and the dead cells allowed us to develop a segregated kinetic model to describe the yeast and the bacteria population dynamics and glucose consumption in batch cultures. This model, more complete than that which is traditionally used, based only on viable cell measurements, describes better the behavior and the functionality of the cultures, giving a deeper knowledge in real time about the status and the course of the bioprocesses.

Intracellular pH evolution during batch cultures using flow cytometry

1994 ◽  
pp. 351-353
Author(s):  
M. Cherlet ◽  
N. Petitpain ◽  
M. Dardenne ◽  
P. Franck ◽  
J.M. Engasser ◽  
...  
Keyword(s):  
Flow Cytometry ◽  
Intracellular Ph ◽  
Batch Cultures

Cell-controlled hybrid perfusion fed-batch CHO cell process provides significant productivity improvement over conventional fed-batch cultures

2017 ◽  
Vol 114 (7) ◽  
pp. 1438-1447 ◽  
Author(s):  
Gregory W. Hiller ◽  
Ana Maria Ovalle ◽  
Matthew P. Gagnon ◽  
Meredith L. Curran ◽  
Wenge Wang
Keyword(s):  
Cell Process ◽  
Fed Batch ◽  
Cho Cell ◽  
Productivity Improvement ◽  
Batch Cultures

Addition of Valproic Acid to CHO Cell Fed-Batch Cultures Improves Monoclonal Antibody Titers

2014 ◽  
Vol 56 (5) ◽  
pp. 421-428 ◽  
Author(s):  
William C. Yang ◽  
Jiuyi Lu ◽  
Ngan B. Nguyen ◽  
An Zhang ◽  
Nicholas V. Healy ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Valproic Acid ◽  
Fed Batch ◽  
Cho Cell ◽  
Batch Cultures ◽  
Antibody Titers

Profiling of N-glycosylation gene expression in CHO cell fed-batch cultures

2010 ◽  
Vol 107 (3) ◽  
pp. 516-528 ◽  
Author(s):  
Danny Chee Furng Wong ◽  
Niki Soo Ching Wong ◽  
John Soo Yang Goh ◽  
Lee May May ◽  
Miranda Gek Sim Yap
Keyword(s):  
Gene Expression ◽  
Fed Batch ◽  
Cho Cell ◽  
Batch Cultures

Automated flow cytometry for monitoring CHO cell cultures

Methods ◽  
2012 ◽  
Vol 56 (3) ◽  
pp. 358-365 ◽  
Author(s):  
Darrin Kuystermans ◽  
Avesh Mohd ◽  
Mohamed Al-Rubeai
Keyword(s):  
Flow Cytometry ◽  
Cell Cultures ◽  
Cho Cell
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