Cell-controlled hybrid perfusion fed-batch CHO cell process provides significant productivity improvement over conventional fed-batch cultures

2017 ◽  
Vol 114 (7) ◽  
pp. 1438-1447 ◽  
Author(s):  
Gregory W. Hiller ◽  
Ana Maria Ovalle ◽  
Matthew P. Gagnon ◽  
Meredith L. Curran ◽  
Wenge Wang
Keyword(s):  
Cell Process ◽  
Fed Batch ◽  
Cho Cell ◽  
Productivity Improvement ◽  
Batch Cultures

Addition of Valproic Acid to CHO Cell Fed-Batch Cultures Improves Monoclonal Antibody Titers

2014 ◽  
Vol 56 (5) ◽  
pp. 421-428 ◽  
Author(s):  
William C. Yang ◽  
Jiuyi Lu ◽  
Ngan B. Nguyen ◽  
An Zhang ◽  
Nicholas V. Healy ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Valproic Acid ◽  
Fed Batch ◽  
Cho Cell ◽  
Batch Cultures ◽  
Antibody Titers

Profiling of N-glycosylation gene expression in CHO cell fed-batch cultures

2010 ◽  
Vol 107 (3) ◽  
pp. 516-528 ◽  
Author(s):  
Danny Chee Furng Wong ◽  
Niki Soo Ching Wong ◽  
John Soo Yang Goh ◽  
Lee May May ◽  
Miranda Gek Sim Yap
Keyword(s):  
Gene Expression ◽  
Fed Batch ◽  
Cho Cell ◽  
Batch Cultures

scholarly journals Metabolic characterization of a CHO cell size increase phase in fed-batch cultures

2017 ◽  
Vol 101 (22) ◽  
pp. 8101-8113 ◽  
Author(s):  
Xiao Pan ◽  
Ciska Dalm ◽  
René H. Wijffels ◽  
Dirk E. Martens
Keyword(s):  
Cell Size ◽  
Size Increase ◽  
Fed Batch ◽  
Cho Cell ◽  
Batch Cultures

scholarly journals Production of Butyrate and Branched Chain Amino Acid Catabolic Byproducts by CHO Cells in Fed-batch Culture Enhances their Specific Productivity

2021 ◽  
Author(s):  
Cameron Harrington ◽  
Taylor Kalomeris ◽  
Michaela Jacobs ◽  
Gregory Hiller ◽  
Bhanu Chandra Mulukutla
Keyword(s):  
Cell Proliferation ◽  
Amino Acid ◽  
Cho Cells ◽  
Lactate Production ◽  
Specific Productivity ◽  
Fed Batch ◽  
Cho Cell ◽  
Batch Cultures ◽  
Branched Chain Amino Acid ◽  
Branched Chain

Chinese hamster ovary (CHO) cells in fed-batch cultures produce several metabolic byproducts derived from amino acid catabolism, some of which accumulate to growth inhibitory levels. Controlling the accumulation of these byproducts has been shown to significantly enhance cell proliferation. Interestingly, some of these byproducts have physiological roles that go beyond inhibition of cell proliferation. In this study, we show that, in CHO cell fed-batch cultures, branched chain amino acid (BCAA) catabolism contributes to the formation of butyrate, a novel byproduct that is also a well-established specific productivity enhancer. Further, the other byproducts of BCAA catabolism, isovalerate and isobutyrate, which accumulate in CHO cell fed-batch cultures also enhance specific productivity. Additionally, the rate of production of these BCAA catabolic byproducts was negatively correlated with glucose uptake and lactate production rates. Limiting glucose supply to suppress glucose uptake and lactate production, like in case of fed-batch cultures employing HiPDOG technology, significantly enhances BCAA catabolic byproduct accumulation resulting in higher specific productivities.

Elucidating apoptotic cell death in cho cell batch & fed-batch cultures

2006 ◽  
pp. 61-66
Author(s):  
Danny C.F. Wong ◽  
C.K.Danny C.F. Wong ◽  
C. K. Heng ◽  
Kathy T. K. Wong ◽  
Peter Morin Nissom ◽  
...  
Keyword(s):  
Cell Death ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Fed Batch ◽  
Cho Cell ◽  
Batch Cultures
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