Estradiol distribution and penetration in rat skin after topical application, studied by high resolution autoradiography

1990 ◽  
Vol 95 (1) ◽  
pp. 43-54 ◽  
Author(s):  
H. J. Bidmon ◽  
J. D. Pitts ◽  
H. F. Solomon ◽  
J. V. Bondi ◽  
W. E. Stumpf
Keyword(s):  
High Resolution ◽  
Topical Application ◽  
Rat Skin ◽  
High Resolution Autoradiography

Gel-based Microemulsion Design and Evaluation for Topical Application of Rivastigmine

2020 ◽  
Vol 21 (4) ◽  
pp. 298-304
Author(s):  
Chih-Wen Fang ◽  
Ling-Chun Tsai ◽  
Yaw-Syan Fu ◽  
Ting-Yu Cheng ◽  
Pao-Chu Wu
Keyword(s):  
Topical Application ◽  
Skin Irritation ◽  
Topical Administration ◽  
Lag Time ◽  
Enhancement Effect ◽  
Microemulsion Formulation ◽  
Rat Skin ◽  
Glycol Monobutyl Ether ◽  
Loading Amount ◽  
Hydrophilic Gel

Objective: The aim of the present study was to design nanocarriers for the topical application of rivastigmine. Methods: The effect of cosurfactants, hydrophilic gel and loading amount on the permeability of rivastigmine through rat skin was evaluated. Skin irritation tests and stability tests were performed to evaluate the utility of tested formulations. Results: The results showed that the microemulsion formation and characteristics of drug-loaded formulations were related to many parameters of the components. When using microemulsion systems as a vehicle, the permeation rate remarkably increased about 13.2~24.3-fold and the lag time was significantly shortened from 24 h to 4.7 h. Formulations containing a cosurfactant of Diethylene Glycol Monobutyl Ether (DEGBE) showed higher enhancement effect, while increasing the loading dose from 0.5% to 5% further increased the flux about 2.1-fold and shortened the lag time. Conclusion: The drug-loaded experimental formulation did not cause skin irritation and had good stability at 20ºC and 40ºC storage for at least 3 months. The result showed that gel-based microemulsion formulation could be a promising approach for topical administration.

scholarly journals The metabolic transformations of columbinic acid and the effect of topical application of the major metabolites on rat skin.

1985 ◽  
Vol 260 (2) ◽  
pp. 987-992
Author(s):  
W J Elliott ◽  
A R Morrison ◽  
H W Sprecher ◽  
P Needleman
Keyword(s):  
Topical Application ◽  
Rat Skin

Mise en évidence du rôle, dans la régénération des Amphibiens, d'une glycoprotéine sécrétée par la cape apicale: étude cytochimique et autoradiographique en microscopie électronique

Development ◽  
1974 ◽  
Vol 32 (1) ◽  
pp. 133-145
Author(s):  
Par Claude Chapron
Keyword(s):  
High Resolution ◽  
Epithelial Cells ◽  
Limb Regeneration ◽  
Target Cells ◽  
Silver Particles ◽  
Electron Microscopic ◽  
Microscopic Studies ◽  
High Resolution Autoradiography

Evidence for the role of an apical cap glycoprotein in amphibian regeneration: cytochemical and autoradiographic electron-microscopic studies Early during limb regeneration in the newt, an ectodermal apical cap covering a mesodermal blastema is formed. High-resolution autoradiography of these tissues has been carried out after incorporation of [3H]fucose, which is a precursor of glycoproteins. Autoradiography shows that silver particles are located at first on epithelial cells, then on mesenchymatous cells. This observation is consistent with a hypothesis in which the apical cap would elaborate a glycoprotein acting on the blastema. Substructural autoradiography and cytochemistry also show the importance of cellular surfaces for both cells producing glycoprotein and those which are target cells.

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