Studies of the feulgen reaction with the aid of DNA incorporated cellulose films

1961 ◽  
Vol 2 (4) ◽  
pp. 283-297 ◽  
Author(s):  
J. -P. Persijn ◽  
P. Duijn
Keyword(s):  
Feulgen Reaction ◽  
Cellulose Films

Localization of DNA in chromatin using ESI and osmium ammine staining

1990 ◽  
Vol 48 (3) ◽  
pp. 116-117
Author(s):  
C.L. Woodcock ◽  
R.A. Horowitz ◽  
D. P. Bazett-Jones ◽  
A.L. Olins
Keyword(s):  
Spectroscopic Imaging ◽  
Energy Losses ◽  
Electron Spectroscopic Imaging ◽  
Feulgen Reaction ◽  
Specific Location ◽  
Eukaryotic Nucleus ◽  
Chromatin Fibers ◽  
Patiria Miniata ◽  
Model Structures

In the eukaryotic nucleus, DNA is packaged into nucleosomes, and the nucleosome chain folded into ‘30nm’ chromatin fibers. A number of different model structures, each with a specific location of nucleosomal and linker DNA have been proposed for the arrangment of nucleosomes within the fiber. We are exploring two strategies for testing the models by localizing DNA within chromatin: electron spectroscopic imaging (ESI) of phosphorus atoms, and osmium ammine (OSAM) staining, a method based on the DNA-specific Feulgen reaction.Sperm were obtained from Patiria miniata (starfish), fixed in 2% GA in 150mM NaCl, 15mM HEPES pH 8.0, and embedded In Lowiciyl K11M at -55C. For OSAM staining, sections 100nm to 150nm thick were treated as described, and stereo pairs recorded at 40,000x and 100KV using a Philips CM10 TEM. (The new osmium ammine-B stain is available from Polysciences Inc). Uranyl-lead (U-Pb) staining was as described. ESI was carried out on unstained, very thin (<30 nm) beveled sections at 80KV using a Zeiss EM902. Images were recorded at 20,000x and 30,000x with median energy losses of 110eV, 120eV and 160eV, and a window of 20eV.

Low permeable hydrophobic nanofibrilated cellulose films modified by dipping and heating processing technique

Cellulose ◽  
2021 ◽  
Author(s):  
Gustavo de Souza ◽  
Mohamed Naceur Belgacem ◽  
Alessandro Gandini ◽  
Antonio José Felix Carvalho
Keyword(s):  
Processing Technique ◽  
Cellulose Films

scholarly journals Bacterial cellulose films with ZnO nanoparticles and propolis extracts: Synergistic antimicrobial effect

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Alexandra Mocanu ◽  
Gabriela Isopencu ◽  
Cristina Busuioc ◽  
Oana-Maria Popa ◽  
Paul Dietrich ◽  
...  
Keyword(s):  
Synergistic Effect ◽  
Bacterial Cellulose ◽  
Food Packaging ◽  
Antimicrobial Effect ◽  
Gas Chromatography Mass Spectrometry ◽  
Trolox Equivalent Antioxidant Capacity ◽  
Zno Nps ◽  
X Ray ◽  
Cellulose Films ◽  
Trolox Equivalent

AbstractThis study aimed to obtain possible materials for future antimicrobial food packaging applications based on biodegradable bacterial cellulose (BC). BC is a fermentation product obtained by Gluconacetobacter xylinum using food or agricultural wastes as substrate. In this work we investigated the synergistic effect of zinc oxide nanoparticles (ZnO NPs) and propolis extracts deposited on BC. ZnO NPs were generated in the presence of ultrasounds directly on the surface of BC films. The BC-ZnO composites were further impregnated with ethanolic propolis extracts (EEP) with different concentrations.The composition of raw propolis and EEP were previously determined by gas-chromatography mass-spectrometry (GC-MS), while the antioxidant activity was evaluated by TEAC (Trolox equivalent antioxidant capacity). The analysis methods performed on BC-ZnO composites such as scanning electron microscopy (SEM), thermo-gravimetrically analysis (TGA), and energy-dispersive X-ray spectroscopy (EDX) proved that ZnO NPs were formed and embedded in the whole structure of BC films. The BC-ZnO-propolis films were characterized by SEM and X-ray photon spectroscopy (XPS) in order to investigate the surface modifications. The antimicrobial synergistic effect of the BC-ZnO-propolis films were evaluated against Escherichia coli, Bacillus subtilis, and Candida albicans. The experimental results revealed that BC-ZnO had no influence on Gram-negative and eukaryotic cells.

scholarly journals Feulgen reaction study of novel threadlike structures (Bonghan ducts) on the surfaces of mammalian organs

2005 ◽  
Vol 284B (1) ◽  
pp. 35-40 ◽  
Author(s):  
Hak-Soo Shin ◽  
Hyeon-Min Johng ◽  
Byung-Cheon Lee ◽  
Sung-Il Cho ◽  
Kyung-Soon Soh ◽  
...  
Keyword(s):  
Feulgen Reaction ◽  
Reaction Study

scholarly journals Effect of hot calendering on physical properties and water vapor transfer resistance of bacterial cellulose films

2016 ◽  
Vol 51 (21) ◽  
pp. 9562-9572 ◽  
Author(s):  
V. L. D. Costa ◽  
A. P. Costa ◽  
M. E. Amaral ◽  
C. Oliveira ◽  
M. Gama ◽  
...  
Keyword(s):  
Water Vapor ◽  
Physical Properties ◽  
Bacterial Cellulose ◽  
Transfer Resistance ◽  
Cellulose Films ◽  
Vapor Transfer ◽  
Water Vapor Transfer

The effect of chemical composition on microfibrillar cellulose films from wood pulps: water interactions and physical properties for packaging applications

Cellulose ◽  
2010 ◽  
Vol 17 (4) ◽  
pp. 835-848 ◽  
Author(s):  
Kelley L. Spence ◽  
Richard A. Venditti ◽  
Orlando J. Rojas ◽  
Youssef Habibi ◽  
Joel J. Pawlak
Keyword(s):  
Chemical Composition ◽  
Physical Properties ◽  
Cellulose Films ◽  
Wood Pulps ◽  
Microfibrillar Cellulose
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