scholarly journals Feulgen reaction study of novel threadlike structures (Bonghan ducts) on the surfaces of mammalian organs

2005 ◽  
Vol 284B (1) ◽  
pp. 35-40 ◽  
Author(s):  
Hak-Soo Shin ◽  
Hyeon-Min Johng ◽  
Byung-Cheon Lee ◽  
Sung-Il Cho ◽  
Kyung-Soon Soh ◽  
...  
Keyword(s):  
Feulgen Reaction ◽  
Reaction Study

Autoradiographic and Feulgen reaction study of the regenerating basal melanocytes of the white guinea-pig

1973 ◽  
Vol 85 (1) ◽  
pp. 37-40 ◽  
Author(s):  
R.C. Shukla ◽  
Madhuri Rastogi ◽  
R.N. Sharma
Keyword(s):  
Guinea Pig ◽  
Feulgen Reaction ◽  
Reaction Study

Localization of DNA in chromatin using ESI and osmium ammine staining

1990 ◽  
Vol 48 (3) ◽  
pp. 116-117
Author(s):  
C.L. Woodcock ◽  
R.A. Horowitz ◽  
D. P. Bazett-Jones ◽  
A.L. Olins
Keyword(s):  
Spectroscopic Imaging ◽  
Energy Losses ◽  
Electron Spectroscopic Imaging ◽  
Feulgen Reaction ◽  
Specific Location ◽  
Eukaryotic Nucleus ◽  
Chromatin Fibers ◽  
Patiria Miniata ◽  
Model Structures

In the eukaryotic nucleus, DNA is packaged into nucleosomes, and the nucleosome chain folded into ‘30nm’ chromatin fibers. A number of different model structures, each with a specific location of nucleosomal and linker DNA have been proposed for the arrangment of nucleosomes within the fiber. We are exploring two strategies for testing the models by localizing DNA within chromatin: electron spectroscopic imaging (ESI) of phosphorus atoms, and osmium ammine (OSAM) staining, a method based on the DNA-specific Feulgen reaction.Sperm were obtained from Patiria miniata (starfish), fixed in 2% GA in 150mM NaCl, 15mM HEPES pH 8.0, and embedded In Lowiciyl K11M at -55C. For OSAM staining, sections 100nm to 150nm thick were treated as described, and stereo pairs recorded at 40,000x and 100KV using a Philips CM10 TEM. (The new osmium ammine-B stain is available from Polysciences Inc). Uranyl-lead (U-Pb) staining was as described. ESI was carried out on unstained, very thin (<30 nm) beveled sections at 80KV using a Zeiss EM902. Images were recorded at 20,000x and 30,000x with median energy losses of 110eV, 120eV and 160eV, and a window of 20eV.

scholarly journals Fasting Blood Glucose and 2-h Postprandial Blood Glucose Predict Hypertension: A Report from the REACTION Study

2021 ◽  
Vol 12 (4) ◽  
pp. 1117-1128
Author(s):  
Yingkui Si ◽  
Anping Wang ◽  
Yunshuang Yang ◽  
Hongzhou Liu ◽  
Shi Gu ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Fasting Blood Glucose ◽  
Postprandial Blood Glucose ◽  
Reaction Study

scholarly journals A Rapid Reaction Study of Anthranilate Hydroxylase

1989 ◽  
Vol 264 (27) ◽  
pp. 16008-16016 ◽  
Author(s):  
J Powlowski ◽  
D Ballou ◽  
V Massey
Keyword(s):  
Reaction Study ◽  
Rapid Reaction

An application of the polarizing microscope to reaction study

1942 ◽  
Vol 19 (7) ◽  
pp. 331 ◽  
Author(s):  
Garrett W. Thiessen ◽  
Lawrence F. Beste
Keyword(s):  
Reaction Study ◽  
Polarizing Microscope

scholarly journals STUDIES ON NUCLEI USING CORRELATED CYTOCHEMICAL, LIGHT, AND ELECTRON MICROSCOPE TECHNIQUES

1956 ◽  
Vol 2 (4) ◽  
pp. 397-406 ◽  
Author(s):  
Montrose J. Moses
Keyword(s):  
Electron Microscope ◽  
Nuclear Envelope ◽  
Light Microscope ◽  
Primary Spermatocyte ◽  
Feulgen Reaction ◽  
Remarkable Feature ◽  
Pas Reaction ◽  
Electron Image ◽  
Careful Comparison ◽  
Positive Nature

In this paper, a procedure for correlating electron microscope and light microscope cytochemical studies using immediately adjacent serial thin and thick sections has been described and discussed. This technique, combined with the Feulgen reaction for DNA, has been of particular value in framing and answering both general and specific questions about the nucleus. The results may be summarized as follows:— Apparent nuclear homogeneity in the electron microscope is not due to loss of DNA as evidenced by positive Feulgen reactions in such nuclei. Arrangement of Feulgen-positive material in chromosomes, heterochromatin, perinuclear and perinucleolar chromatin, etc., is similar to that customarily observed in the light microscope but this is not necessarily reflected in a cursory survey of the electron image. Careful comparison of light and electron images shows that fine differences in structure are associated with chromatin localization. Primary spermatocyte prophase chromosomes of crayfish have been positively identified by their Feulgen-positive nature. Core-like axial structures in such chromosomes have been observed (9) and are described further. A remarkable feature of spermiogenesis in the crayfish is an elaboration of the nuclear envelope of the spermatid accompanying the formation of what becomes a mass of convoluted membranes in the sperm. In the spermatid, perinuclear chromatin follows outpocketings of the nuclear envelope into the cytoplasm. In the early sperm, on the other hand, although the nuclear envelope is continuous with the system of convoluted membranes, the chromatin is distinct from it and is retained in the nucleus proper by some mechanism independent of the nuclear envelope. None of the above observations was apparent from the electron microscope images alone; they were possible only by virtue of the correlated cytochemical and electron microscope study of adjacent sections. The successful use of other cytochemical tests, such as the PAS reaction for certain carbohydrates, in such correlated studies is also described.

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