Determination of the amount of aromatic hydrocarbons in petroleum fractions by means of gas-liquid chromatography

1965 ◽  
Vol 1 (7) ◽  
pp. 525-528
Author(s):  
R. I. Sidorov ◽  
A. N. Denisenko ◽  
M. P. Ivanova ◽  
L. A. Polyakova ◽  
I. N. Agapova
Keyword(s):  
Liquid Chromatography ◽  
Aromatic Hydrocarbons ◽  
Gas Liquid Chromatography ◽  
Petroleum Fractions

Determination of N-Alkane and Methylnaphthalene Compounds in Shellfish

1973 ◽  
Vol 1973 (1) ◽  
pp. 173-177 ◽  
Author(s):  
J. W. Blaylock ◽  
P. W. O'Keefe ◽  
J. N. Roehm ◽  
R. E. Wildung
Keyword(s):  
Liquid Chromatography ◽  
Column Chromatography ◽  
Aromatic Hydrocarbons ◽  
Internal Standard ◽  
Gas Liquid Chromatography ◽  
Marine Biota ◽  
Tissue Extracts ◽  
Quantitative Estimates ◽  
Petroleum Residues

ABSTRACT During the course of investigations to determine the possible toxicity of petroleum to marine biota, it became evident that quantitative estimates of the petroleum components in water and biota would assist in meaningful interpretation of the results of bioassays. However, published procedures for estimation of n-alkanes in marine biota were largely qualitative, and even less effort had been afforded the measurement of aromatic petroleum residues. A method originally utilized for determination of poly cyclic aromatic hydrocarbons in foods was therefore adapted for the digestion of tissue and extraction of hydrocarbons from shellfish exposed to petroleum during bioassays. Tissue extracts were partitioned into saturate and aromatic fractions by column chromatography. Using gas-liquid chromatography, the n-alkanes of carbon numbers 12 to 19, and the methyl substituted naphthalenes were identified in the saturate and aromatic fractions, respectively. Both groups of compounds were quantitated by reference to an internal standard. The procedure allowed recovery of over 70 percent of n-alkanes and methylnaphthalenes applied to the tissues prior to digestion. Minimum detectable levels for n-alkanes and methylnaphthalenes were approximately 0.08 to 0.15 and 0.03 to 0.04 µg/g of wet tissue, respectively.

GASCHROMATOGRAPHIC DETERMINATION OF STEROIDS IN THE URINE OF PATIENTS WITH CUSHING'S SYNDROME

1971 ◽  
Vol 67 (2) ◽  
pp. 303-315 ◽  
Author(s):  
A. J. Moolenaar ◽  
A. P. van Seters
Keyword(s):  
Liquid Chromatography ◽  
Cushing’S Syndrome ◽  
Normal Subjects ◽  
Diagnostic Value ◽  
Cushing's Syndrome ◽  
Gas Liquid Chromatography ◽  
Obese Subjects

ABSTRACT The 17-oxosteroids were estimated in the urine of 27 patients with Cushing's syndrome by gas-liquid chromatography (G. L. C.). The values of the various steroid fractions are compared with those of normal subjects, patients with thyrotoxicosis and obese subjects. The effect of the age of the patients on the diagnostic value of the invidual 17-oxosteroids and their ratios is discussed.

Simultaneous Determination of Lactulose and Mannitol in Urine of Burn Patients by Gas-Liquid Chromatography

1992 ◽  
Author(s):  
Ronald L. Shippee ◽  
Avery A. Johnson ◽  
William G. Cioffi ◽  
James Lasko ◽  
Thomas E. LeVoyer
Keyword(s):  
Liquid Chromatography ◽  
Simultaneous Determination ◽  
Gas Liquid Chromatography ◽  
Burn Patients

QuEChERS with ultrasound-assisted extraction combined with high-performance liquid chromatography for the determination of 16 polycyclic aromatic hydrocarbons in sediment

2021 ◽  
Author(s):  
Ling Wu ◽  
Qiurong He ◽  
Jing Zhang ◽  
Yongxin Li ◽  
Weiqing Yang ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Aromatic Hydrocarbons ◽  
High Performance ◽  
Ultrasound Assisted Extraction ◽  
Sediment Samples ◽  
Assisted Extraction ◽  
Polycyclic Aromatic ◽  
Ultrasound Assisted

Abstract Background Polycyclic aromatic hydrocarbons (PAHs) have attracted worldwide attention due to their carcinogenic, teratogenic and mutagenic effects, environmental persistence and bioaccumulation characteristics. Therefore, the sensitive, reliable and rapid detection of PAHs in sediment is of great importance. Objective To develop a high-performance liquid chromatography (HPLC) with fluorescence and ultraviolet detection after QuEChERS treatment for simultaneous determination of 16 U.S. Environmental Protection Agency priority PAHs in sediment samples. Methods The samples were ultrasonically extracted with acetone and then the supernatant was purified with a modified QuEChERS method. After centrifugation, the supernatant was injected into the HPLC system for analysis. The separation was accomplished on a ZORBAX Eclipse PAH column (150 × 4.6 mm, 3.5 μm) and the column temperature was set at 30 °C. The flow rate of the mobile phase consisting of water and acetonitrile in gradient elution mode was fixed at 0.9 mL/min. Detection was conducted on an ultraviolet detector and a fluorescence detector simultaneously. The qualitative analysis was based on retention time and the quantification was based on standard curves. Results Under the optimal conditions, this method showed good linearities in the range of 10–200 μg/L with correlation coefficients greater than 0.9993. The method had the limits of detection (LODs) ranging from 0.00108 to 0.314 ng/g. The mean recoveries ranged from 78.4 to 117% with the intra-day and inter-day relative standard deviations (RSDs) of 0.592–10.7 and 1.01–13.0%, respectively. The proposed method was successfully applied to the detection of 16 PAHs in sediment samples collected from the Funan River in Chengdu, China with the total contents of 431 to 2143 ng/g·dw. Conclusions The established method is simple, rapid, environment-friendly and cost- effective. It can be applied to the analysis of 16 PAHs in sediment samples. Highlights A method of QuEChERS with ultrasound-assisted extraction combined with HPLC has been established for the analysis of 16 PAHs in sediment samples and the proposed method has been successfully applied to the analysis PAHs in real sediment samples.

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