Fluorescence techniques for probing water penetration into lipid bilayers

1995 ◽  
Vol 5 (1) ◽  
pp. 19-28 ◽  
Author(s):  
Christopher D. Stubbs ◽  
Cojen Ho ◽  
Simon J. Slater
Keyword(s):  
Lipid Bilayers ◽  
Water Penetration ◽  
Fluorescence Techniques

scholarly journals Nature and Size of Ganglioside GM1 Nano-Domains in Lipid Bilayers as Revealed by Advanced Time-Resolved Fluorescence Techniques

2015 ◽  
Vol 108 (2) ◽  
pp. 79a
Author(s):  
Radek Sachl ◽  
Mariana Amaro ◽  
Alena Koukalova ◽  
Gockan Aydogan ◽  
Ilya Mikhalyov ◽  
...  
Keyword(s):  
Lipid Bilayers ◽  
Time Resolved Fluorescence ◽  
Ganglioside Gm1 ◽  
Time Resolved ◽  
Fluorescence Techniques

scholarly journals Lipid peroxidation and water penetration in lipid bilayers: A W-band EPR study

2013 ◽  
Vol 1828 (2) ◽  
pp. 510-517 ◽  
Author(s):  
Elena Conte ◽  
Francesco Maria Megli ◽  
Himanshu Khandelia ◽  
Gunnar Jeschke ◽  
Enrica Bordignon
Keyword(s):  
Lipid Peroxidation ◽  
Lipid Bilayers ◽  
Water Penetration ◽  
W Band

Dynamic structure of lipid bilayers studied by nanosecond fluorescence techniques

Biochemistry ◽  
1977 ◽  
Vol 16 (11) ◽  
pp. 2319-2324 ◽  
Author(s):  
Suguru Kawato ◽  
Kazuhiko Kinosita ◽  
Akira Ikegami
Keyword(s):  
Lipid Bilayers ◽  
Dynamic Structure ◽  
Fluorescence Techniques

Observation of Concanavalin-A receptors on hydrated planar erythrocyte membrane film by in situ electron microscopy

1983 ◽  
Vol 41 ◽  
pp. 608-609
Author(s):  
Neng-Bo He ◽  
S.W. Hui
Keyword(s):  
Lipid Bilayers ◽  
Erythrocyte Membrane ◽  
Lipid Membranes ◽  
Surface Film ◽  
Biological Membranes ◽  
Electron Microscopic Observation ◽  
Electron Microscopic ◽  
Black Lipid Membranes ◽  
Fine Mesh ◽  
Planar Films

Monolayers and planar "black" lipid membranes have been widely used as models for studying the structure and properties of biological membranes. Because of the lack of a suitable method to prepare these membranes for electron microscopic observation, their ultrastructure is so far not well understood. A method of forming molecular bilayers over the holes of fine mesh grids was developed by Hui et al. to study hydrated and unsupported lipid bilayers by electron diffraction, and to image phase separated domains by diffraction contrast. We now adapted the method of Pattus et al. of spreading biological membranes vesicles on the air-water interfaces to reconstitute biological membranes into unsupported planar films for electron microscopic study. hemoglobin-free human erythrocyte membrane stroma was prepared by hemolysis. The membranes were spreaded at 20°C on balanced salt solution in a Langmuir trough until a surface pressure of 20 dyne/cm was reached. The surface film was repeatedly washed by passing to adjacent troughs over shallow partitions (fig. 1).

Effect of Integral Proteins on Frozen Membrane Fracture

1980 ◽  
Vol 38 ◽  
pp. 756-759 ◽  
Author(s):  
S. Kirchanski ◽  
D. Branton
Keyword(s):  
Lipid Bilayers ◽  
Freeze Fracture ◽  
Covalent Bond ◽  
Erythrocyte Membranes ◽  
Glycophorin A ◽  
Experimental Protocol ◽  
Transmembrane Glycoprotein ◽  
Bond Breakage ◽  
Human Erythrocyte Membranes ◽  
Integral Proteins

We have investigated the effect of integral membrane proteins upon the fracturing of frozen lipid bilayers. This investigation has been part of an effort to develop freeze fracture labeling techniques and to assess the possible breakage of covalent protein bonds during the freeze fracture process. We have developed an experimental protocol utilizing lectin affinity columns which should detect small amounts of covalent bond breakage during the fracture of liposomes containing purified (1) glycophorin (a transmembrane glycoprotein of human erythrocyte membranes). To fracture liposomes in bulk, frozen liposomes are ground repeatedly under liquid nitrogen. Failure to detect any significant covalent bond breakage (contrary to (2)) led us to question the effectiveness of our grinding procedure in fracturing and splitting lipid bilayers.

Late Stages of the “Pearling" Instability in Lipid Bilayers

1997 ◽  
Vol 7 (9) ◽  
pp. 1185-1204 ◽  
Author(s):  
J. L. Coveas ◽  
S. T. Milner ◽  
W. B. Russel
Keyword(s):  
Lipid Bilayers ◽  
Late Stages
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