A combined myosin ATPase and acetylcholinesterase histochemical method for the demonstration of fibre types and their innervation pattern in skeletal muscle

1993 ◽  
Vol 99 (5) ◽  
pp. 369-372 ◽  
Author(s):  
Joan R. Torrella ◽  
Vicente Fouces ◽  
Jesús Palomeque ◽  
Ginés Viscor
Keyword(s):  
Skeletal Muscle ◽  
Myosin Atpase ◽  
Fibre Types ◽  
Histochemical Method ◽  
Innervation Pattern

Histochemical differentiation of skeletal muscle in foetal and newborn mice

Development ◽  
1964 ◽  
Vol 12 (4) ◽  
pp. 759-767
Author(s):  
C. Wirsén ◽  
K. S. Larsson
Keyword(s):  
Skeletal Muscle ◽  
Muscle Development ◽  
Good Deal ◽  
Fibre Types ◽  
Muscle Bundle ◽  
Adult Skeletal Muscle ◽  
Newborn Mice ◽  
Longitudinal Splitting ◽  
Morphological Criteria ◽  
New Generation

In earlier investigations of muscle development, morphological criteria, such as diameter and staining with routine methods, have been used for classifying different fibre types. In human foetal muscle three fibre sizes are seen from the 15th week (Cuajunco, 1942). The largest fibres seem to be the centre of each primary muscle bundle. They were denoted as B fibres by Wohlfart (1937), who considered that they were also functionally different from the smaller ones forming around them. Tello (1922) and Cuajunco (1942) supported the widely held opinion that the smaller fibres are formed from the larger ones by longitudinal splitting. However, Couteaux (1941) claimed that the small fibres belong to a new generation differentiating from interstitial cells. Histochemical studies on foetal muscle are rare. However, during the last years a good deal of work has been carried out on the histochemistry of adult skeletal muscle.

scholarly journals Phenotypic Modulation of Skeletal Muscle Fibers in LPIN1-Deficient Lipodystrophic (fld) Mice

2018 ◽  
Vol 56 (2) ◽  
pp. 322-331
Author(s):  
Rani S. Sellers ◽  
S. Radma Mahmood ◽  
Geoffrey S. Perumal ◽  
Frank P. Macaluso ◽  
Irwin J. Kurland
Keyword(s):  
Electron Microscopy ◽  
Skeletal Muscle ◽  
Fiber Type ◽  
Periodic Acid ◽  
Muscle Fibers ◽  
Myosin Atpase ◽  
Hybrid Fibers ◽  
Periodic Acid Schiff ◽  
Skeletal Muscle Fibers ◽  
Lipin 1

Lipin-1 ( Lpin1)–deficient lipodystrophic mice have scant and immature adipocytes and develop transient fatty liver early in life. Unlike normal mice, these mice cannot rely on stored triglycerides to generate adenosine triphosphate (ATP) from the β-oxidation of fatty acids during periods of fasting. To compensate, these mice store much higher amounts of glycogen in skeletal muscle and liver than wild-type mice in order to support energy needs during periods of fasting. Our studies demonstrated that there are phenotypic changes in skeletal muscle fibers that reflect an adaptation to this unique metabolic situation. The phenotype of skeletal muscle (soleus, gastrocnemius, plantaris, and extensor digitorum longus [EDL]) from Lpin1-/- was evaluated using various methods including immunohistochemistry for myosin heavy chains (Myh) 1, 2, 2a, 2b, and 2x; enzyme histochemistry for myosin ATPase, cytochrome-c oxidase (COX), and succinyl dehydrogenase (SDH); periodic acid–Schiff; and transmission electron microscopy. Fiber-type changes in the soleus muscle of Lpin1-/- mice were prominent and included decreased Myh1 expression with concomitant increases in Myh2 expression and myosin-ATPase activity; this change was associated with an increase in the presence of Myh1/2a or Myh1/2x hybrid fibers. Alterations in mitochondrial enzyme activity (COX and SDH) were apparent in the myofibers in the soleus, gastrocnemius, plantaris, and EDL muscles. Electron microscopy revealed increases in the subsarcolemmal mitochondrial mass in the muscles of Lpin1-/- mice. These data demonstrate that lipin-1 deficiency results in phenotypic fiber-specific modulation of skeletal muscle necessary for compensatory fuel utilization adaptations in lipodystrophy.

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