Chromosome elimination and mutation in tetraploid somatic hybrids ofSolanum tuberosum andSolanum phureja

1989 ◽  
Vol 8 (2) ◽  
pp. 82-85 ◽  
Author(s):  
Laas P. Pijnacker ◽  
Margriet A. Ferwerda ◽  
Klaas J. Puite ◽  
Jan G. Schaart
Keyword(s):  
Somatic Hybrids ◽  
Chromosome Elimination

Identification of alien chromosomes through GISH and RFLP analysis and the potential for establishing potato lines with monosomic additions of tomato chromosomes

Genome ◽  
1997 ◽  
Vol 40 (5) ◽  
pp. 666-673 ◽  
Author(s):  
F. Garriga-Calderé ◽  
D. J. Huigen ◽  
F. Filotico ◽  
E. Jacobsen ◽  
M. S. Ramanna
Keyword(s):  
In Situ Hybridization ◽  
Somatic Hybrids ◽  
Rflp Analysis ◽  
Chromosome Elimination ◽  
Variable Number ◽  
Substitution Lines ◽  
Chromosome Addition ◽  
Tomato Chromosome ◽  
Complete Series

To increase the potential for establishing a complete series of tomato chromosome addition–sbstitution lines in a potato background, six new BC1 progeny were produced. All of them originated from crosses between three different hexaploid potato (+) tomato fusion hybrids. Three different somatic hybrids, viz., C31-17-5, C31-17-24, and C31-17-51, were used as female parents, and four different tetraploids, viz., Katahdin, Frieslander, 6704-1, and AM66.42 were used as male parents. A characterisation of the genomes of the three fusion hybrids and the six BC1 progenies (6739, 2001, 2002, 2003, 2004, and 2005) through genomic in situ hybridization and restriction fragment length polymorphism (RFLP) analysis indicated that there was preferential tomato chromosome elimination in the fusion hybrids. Similar analyses of the six BC1 progeny indicated that a variable number of the alien tomato chromosomes (6–11) were present in individual plants. RFLP analysis using chromosome specific DNA probes indicated that BC1 progenies had retained all 12 tomato chromosomes, albeit in different individual plants. This means that the available BC1 progenies have the potential for establishing a complete series of tomato chromosome addition–substitution lines in a potato background.Key words: protoplast fusion hybrids, Solanum tuberosum, Lycopersicon esculentum, BC1 progeny, in situ hybridization, RFLP analysis.

scholarly journals ASSESSMENT OF THE LIMITATIONS OF SOMATIC HYBIDIZATION

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1164c-1164
Author(s):  
Cheol Hee Lee ◽  
Kee Yoeup Paek ◽  
J. Brian Power ◽  
Edward C. Cocking
Keyword(s):  
Cell Lines ◽  
Somatic Hybrid ◽  
Somatic Hybridization ◽  
Somatic Hybrids ◽  
Genetic Manipulation ◽  
Hybrid Cell ◽  
Chromosome Elimination ◽  
Essential Information ◽  
Somatic Incompatibility ◽  
Biochemical Analyses

This study was designed to assess the general limitations of somatic hybridization as one of the key technologies for genetic manipulation in plants. The limits of somatic hybridization against different taxonomic backgrounds, intraspecific to interfamilial, were also assessed. Protoplast culture studies provided essential information relating to the species cultural and morphogenetic capacity. several #elect Ion strategies for the recovery of somatic hybrid colonies/plants were developed and assessed using various combinations of protoplast sources and species in the genera Petunia, Nicotiana, Salpiglossis and Chrysanthemum. Morphological, cytological and biochemical analyses were performed to confirm the hybridity of plants or cell lines recovered following protoplasm fusion (using 4-5 methods) and selection.The somatic hybrid callus/plants were obtained at intraspecific to interfamilial levels by complementation to chlorophyll proficiency, together with media selection or complementation of nitrate reductase deficient mutants as follows; P. Hybrida var. Monsanto (+) P. hybrida cv. Blue Lace (intraspecific), P. hybrida var. Monsanto (+) P. inflata and P. parviflora (interspecific), P. parviflora (+) N. tabacum (intergeneric), S. sinuata (+) P. hybrida var. Monsanto, P. parodii and N. tabacum (intertribal), and C. morifolium (+) S. sinuata.From this study, it appeared that there were no taxonomic limits to the production and proliferation of somatic hybrid cell lines. However, obtaining morphologically normal hybrid plants met with limited success as the taxonomic relationships became more distant. The regeneration capacity of somatic hybrids seemed to be controlled by both parental species. Somatic incompatibility mechanism was also shown to operate on chromosome elimination. Such chromosome elimination may well be advantageous in plant improvement.

Genetical Studies on Progenies of Somatic Hybrids between Brassica napus and Descurainia Sophia

2009 ◽  
Vol 35 (6) ◽  
pp. 1044-1050
Author(s):  
Ru-Ying XIN ◽  
Rong-Zhan GUAN ◽  
Li-Jun ZHANG ◽  
Shu-Hui JIANG ◽  
Hong-Sheng ZHANG ◽  
...  
Keyword(s):  
Brassica Napus ◽  
Somatic Hybrids ◽  
Descurainia Sophia

scholarly journals Genetic composition of interspecific potato somatic hybrids and autofused 4x plants evaluated by DArT and cytoplasmic DNA markers

2016 ◽  
Vol 35 (6) ◽  
pp. 1345-1358 ◽  
Author(s):  
Paulina Smyda-Dajmund ◽  
Jadwiga Śliwka ◽  
Iwona Wasilewicz-Flis ◽  
Henryka Jakuczun ◽  
Ewa Zimnoch-Guzowska
Keyword(s):  
Dna Markers ◽  
Somatic Hybrids ◽  
Genetic Composition ◽  
Cytoplasmic Dna

Biased chloroplast and mitochondrial transmission in somatic hybrids of Allium ampeloprasum L. and Allium cepa L.

Plant Science ◽  
1998 ◽  
Vol 131 (2) ◽  
pp. 219-228 ◽  
Author(s):  
J Buiteveld ◽  
W Kassies ◽  
R Geels ◽  
M.M van Lookeren Campagne ◽  
E Jacobsen ◽  
...  
Keyword(s):  
Allium Cepa ◽  
Somatic Hybrids ◽  
Allium Ampeloprasum ◽  
Allium Cepa L ◽  
Mitochondrial Transmission
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