scholarly journals Effects of trypsin on secretion stimulated by micromolar Ca2+ and phorbol ester in digitonin-permeabilized adrenal chromaffin cells

1988 ◽  
Vol 8 (1) ◽  
pp. 115-128 ◽  
Author(s):  
Ronald W. Holz ◽  
Ruth A. Senter
Keyword(s):  
Phorbol Ester ◽  
Chromaffin Cells ◽  
Adrenal Chromaffin Cells ◽  
Adrenal Chromaffin

Neurofilament Phosphorylation in Cultured Bovine Adrenal Chromaffin Cells Is Stimulated by Phorbol Ester

1989 ◽  
Vol 52 (4) ◽  
pp. 1156-1161 ◽  
Author(s):  
Elias Georges ◽  
Michael H. Lindenbaum ◽  
Michael G. Sacher ◽  
José-María Trifaró ◽  
Walter E. Mushynski
Keyword(s):  
Phorbol Ester ◽  
Chromaffin Cells ◽  
Adrenal Chromaffin Cells ◽  
Neurofilament Phosphorylation ◽  
Adrenal Chromaffin

Biphasic Generation of Diacylglycerol by Angiotensin and Phorbol Ester in Bovine Adrenal Chromaffin Cells

1993 ◽  
Vol 190 (1) ◽  
pp. 181-185 ◽  
Author(s):  
R.K. Tuominen ◽  
M.H. Werner ◽  
H. Ye ◽  
M.K. Mcmillian ◽  
P.M. Hudson ◽  
...  
Keyword(s):  
Phorbol Ester ◽  
Chromaffin Cells ◽  
Adrenal Chromaffin Cells ◽  
Adrenal Chromaffin

Regulations by cyclic nucleotides and phorbol ester of calcium efflux from cultured bovine adrenal chromaffin cells

Life Sciences ◽  
1995 ◽  
Vol 57 (15) ◽  
pp. PL211-PL215 ◽  
Author(s):  
Hitoshi Houchi ◽  
Masaaki Okuno ◽  
Masayuki Shono ◽  
Akira Tokumura ◽  
Motoo Oka
Keyword(s):  
Phorbol Ester ◽  
Cyclic Nucleotides ◽  
Chromaffin Cells ◽  
Adrenal Chromaffin Cells ◽  
Calcium Efflux ◽  
Adrenal Chromaffin

scholarly journals Inhibition of prostaglandin E2-induced phosphoinositide metabolism by phorbol ester in bovine adrenal chromaffin cells

1988 ◽  
Vol 255 (3) ◽  
pp. 957-962 ◽  
Author(s):  
H Yokohama ◽  
M Negishi ◽  
K Sugama ◽  
H Hayashi ◽  
S Ito ◽  
...  
Keyword(s):  
Prostaglandin E2 ◽  
Phorbol Ester ◽  
Chromaffin Cells ◽  
Inositol Phosphates ◽  
Phosphoinositide Metabolism ◽  
Adrenal Chromaffin Cells ◽  
Gtp Binding Protein ◽  
Intact Cells ◽  
Gtp Binding ◽  
Adrenal Chromaffin

In bovine adrenal chromaffin cells, prostaglandin E2 (PGE2) stimulates the formation of inositol phosphates and Ca2+ mobilization through its specific receptor [Yokohama, Tanaka, Ito, Negishi, Hayashi & Hayaishi (1988) J. Biol. Chem. 263, 1119-1122]. Here we show that PGE2-induced phosphoinositide metabolism was blocked by pretreatment with 12-O-tetradecanoylphorbol 13-acetate (TPA). Using intact cells, we also examined the inhibitory effect of TPA on the individual steps of the activation process of phosphoinositide metabolism. The inhibition was observed within 1 min and complete by 10 min after addition of 1 microM-TPA, and half-maximal inhibition by TPA occurred at 20 nM. TPA prevented Ca2+ mobilization induced by PGE2, but not by the Ca2+ ionophore ionomycin. The inactive phorbol ester 4 alpha-phorbol 12,13-didecanoate did not inhibit the formation of inositol phosphates and Ca2+ mobilization by PGE2. TPA treatment affected neither the high-affinity binding of [3H]PGE2 to intact cells and membrane fractions nor the ability of guanosine 5′-[gamma-thio]triphosphate to decrease the binding in membrane fractions. TPA also abolished phosphoinositide metabolism induced by muscarinic-receptor activation. NaF plus AlCl3 and ionomycin caused the accumulation of inositol phosphates, probably by directly activating a GTP-binding protein(s) and phospholipase C respectively; neither accumulation was inhibited by TPA treatment. These results suggest that protein kinase C serves as a feedback regulator for PGE2-induced phosphoinositide metabolism. The site of action of TPA appears to be distal to the coupling of the receptor to GTP-binding protein, but on a component(s) specific to the agonist-induced phosphoinositide metabolism.

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