Iron supply and hydrogenase activity in green algae

1968 ◽  
Vol 61 (1) ◽  
pp. 77-80 ◽  
Author(s):  
Erich Kessler
2012 ◽  
Vol 512-515 ◽  
pp. 1479-1483 ◽  
Author(s):  
Feng Ping Miao ◽  
Jing Cheng Zuo ◽  
Zheng Yi Liu ◽  
Song Qin

In this study, four strains of Chlorella genus and four strains of Tetraselmis genus were investigated to research their in vitro hydrogenase activities and hydrogen productions. C.sp.-3 showed the second highest in vitro hydrogenase activity with 297.48 nmol H2/(μg Chla h) and the highest volume of H2 production with 10.246 μl/L among all the strains. Although T. sp.-3 exhibited a much low H2 production of 0.298 μl/L, its in vitro hydrogenase activity was the highest with 315.92 nmol H2/(μg Chla h). During the continuous culture of five weeks, the hydrogen production of C. sp.-3 reached the peak at 3rd weeks with 12.46 μl H2 per liter culture, and decreased subsequently. In contrast, that of T. sp.-3 increased slowly and gradually with the culturing time, and was much lower than that of C. sp.-3 at each culture phases. These results showed that hydrogen production was a complex process that was determined not only by strain types but also by other factors, and that both C.sp.-3 and T. sp.-3 in the 8 strains were the most promising ones in hydrogen production and were worthy of further research.


Author(s):  
L. V. Leak

Electron microscopic observations of freeze-fracture replicas of Anabaena cells obtained by the procedures described by Bullivant and Ames (J. Cell Biol., 1966) indicate that the frozen cells are fractured in many different planes. This fracturing or cleaving along various planes allows one to gain a three dimensional relation of the cellular components as a result of such a manipulation. When replicas that are obtained by the freeze-fracture method are observed in the electron microscope, cross fractures of the cell wall and membranes that comprise the photosynthetic lamellae are apparent as demonstrated in Figures 1 & 2.A large portion of the Anabaena cell is composed of undulating layers of cytoplasm that are bounded by unit membranes that comprise the photosynthetic membranes. The adjoining layers of cytoplasm are closely apposed to each other to form the photosynthetic lamellae. Occassionally the adjacent layers of cytoplasm are separated by an interspace that may vary in widths of up to several 100 mu to form intralamellar vesicles.


Author(s):  
A. E. Hotchkiss ◽  
A. T. Hotchkiss ◽  
R. P. Apkarian

Multicellular green algae may be an ancestral form of the vascular plants. These algae exhibit cell wall structure, chlorophyll pigmentation, and physiological processes similar to those of higher plants. The presence of a vascular system which provides water, minerals, and nutrients to remote tissues in higher plants was believed unnecessary for the algae. Among the green algae, the Chaetophorales are complex highly branched forms that might require some means of nutrient transport. The Chaetophorales do possess apical meristematic groups of cells that have growth orientations suggestive of stem and root positions. Branches of Chaetophora incressata were examined by the scanning electron microscope (SEM) for ultrastructural evidence of pro-vascular transport.


1965 ◽  
Vol 48 (2) ◽  
pp. 263-271 ◽  
Author(s):  
Herbert Schriefers ◽  
Gerlinde Scharlau ◽  
Franzis Pohl

ABSTRACT After the administration of anabolic steroids to adult female rats in daily doses of 1 mg per animal for 14 days, the following parameters were investigated: the rate of the Δ4-5α-hydrogenase-catalyzed cortisone reduction in liver slices and microsomal fractions, the adrenal weight and the in vitro corticosterone production rate. Among the steroids tested, only 17α-methyl-testosterone and 17α-ethyl-19-nor-testosterone were effective in lowering significantly cortisone reduction rate by liver slices with concomitant decreases in microsomal Δ4-5α-hydrogenase-activity. Testosterone, 19-nor-testosterone, 17α-ethinyl-19-nor-testosterone, 17α-methyl-17β-hydroxy-androsta-1,4-dien-3-one and 1-methyl-17β-hydroxy-androst-1-en-3-one were ineffective or only slightly effective. Adrenal weight and absolute corticosterone production rate (μg/60 min per animal) were decreased after treatment with 17α-methyl-testosterone, 17α-ethyl-19-nor-testosterone and 1-methyl-17β-hydroxy-androst-1-en-3-one. Corticosterone production was decreased with 17α-ethinyl-19-nor-testosterone in spite of an unchanged adrenal weight. The relative corticosterone production rate (μg/60 min · 100 mg adrenal) was in any cases unaffected. According to these results there exists – with the exception of 17α-ethinyl-19-nor-testosterone – a strict parallelism between corticosteroid turnover and corticosterone production rate: unchanged turnover is correlated with unchanged corticosterone production rate, while a decreased turnover is correlated with decreased adrenal activity. The protein-anabolic effect of certain anabolic steroids may be partly due to an anti-catabolic action of these compounds resulting from a decreased corticosteroid inactivation and production rate. Possible mechanisms by which anabolic steroids may affect corticosteroid-balance are discussed.


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