Role of electron-donating cosubstrates in the anaerobic biotransformation of chlorophenoxyacetates to chlorophenols by a bacterial consortium enriched on phenoxyacetate

1993 ◽  
Vol 4 (1) ◽  
pp. 51-57 ◽  
Author(s):  
Susan A. Gibson ◽  
Joseph M. Suflita
Keyword(s):  
Bacterial Consortium ◽  
Anaerobic Biotransformation

scholarly journals Functional Redundancy of Linuron Degradation in Microbial Communities in Agricultural Soil and Biopurification Systems

2016 ◽  
Vol 82 (9) ◽  
pp. 2843-2853 ◽  
Author(s):  
Benjamin Horemans ◽  
Karolien Bers ◽  
Erick Ruiz Romero ◽  
Eva Pose Juan ◽  
Vincent Dunon ◽  
...  
Keyword(s):  
Agricultural Soil ◽  
Bacterial Consortium ◽  
Bacterial Degradation ◽  
Catabolic Gene ◽  
Bacterial Populations ◽  
Content Type ◽  
Respective Contribution ◽  
On Farm ◽  
Biopurification Systems

ABSTRACTThe abundance oflibA, encoding a hydrolase that initiates linuron degradation in the linuron-metabolizingVariovoraxsp. strain SRS16, was previously found to correlate well with linuron mineralization, but not in all tested environments. Recently, an alternative linuron hydrolase, HylA, was identified inVariovoraxsp. strain WDL1, a strain that initiates linuron degradation in a linuron-mineralizing commensal bacterial consortium. The discovery of alternative linuron hydrolases poses questions about the respective contribution and competitive character ofhylA- andlibA-carrying bacteria as well as the role of linuron-mineralizing consortia versus single strains in linuron-exposed settings. Therefore, dynamics ofhylAas well asdcaQas a marker for downstream catabolic functions involved in linuron mineralization, in response to linuron treatment in agricultural soil and on-farm biopurification systems (BPS), were compared with previously reportedlibAdynamics. The results suggest that (i) organisms containing eitherlibAorhylAcontribute simultaneously to linuron biodegradation in the same environment, albeit to various extents, (ii) environmental linuron mineralization depends on multispecies bacterial food webs, and (iii) initiation of linuron mineralization can be governed by currently unidentified enzymes.IMPORTANCEA limited set of different isofunctional catabolic gene functions is known for the bacterial degradation of the phenylurea herbicide linuron, but the role of this redundancy in linuron degradation in environmental settings is not known. In this study, the simultaneous involvement of bacteria carrying one of two isofunctional linuron hydrolysis genes in the degradation of linuron was shown in agricultural soil and on-farm biopurification systems, as was the involvement of other bacterial populations that mineralize the downstream metabolites of linuron hydrolysis. This study illustrates the importance of the synergistic metabolism of pesticides in environmental settings.

Role of identified bacterial consortium in treatment of Quhafa Wastewater Treatment Plant influent in Fayuom, Egypt

2020 ◽  
Vol 192 (3) ◽  
Author(s):  
Salma Ibrahim ◽  
Mohamed Azab El-Liethy ◽  
Khalid Z. Elwakeel ◽  
Mohammed Abd El-Gabbar Hasan ◽  
Ali Mahmoud Al Zanaty ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Wastewater Treatment Plant ◽  
Treatment Plant ◽  
Bacterial Consortium

scholarly journals Synergistic Degradation of Linuron by a Bacterial Consortium and Isolation of a Single Linuron-Degrading Variovorax Strain

2003 ◽  
Vol 69 (3) ◽  
pp. 1532-1541 ◽  
Author(s):  
Winnie Dejonghe ◽  
Ellen Berteloot ◽  
Johan Goris ◽  
Nico Boon ◽  
Katrien Crul ◽  
...  
Keyword(s):  
Denaturing Gradient Gel Electrophoresis ◽  
Enrichment Culture ◽  
Bacterial Community Composition ◽  
Sole Source ◽  
Bacterial Consortium ◽  
The Other ◽  
Rrna Gene ◽  
Comamonas Testosteroni ◽  
Isolation And Identification

ABSTRACT The bacterial community composition of a linuron-degrading enrichment culture and the role of the individual strains in linuron degradation have been determined by a combination of methods, such as denaturing gradient gel electrophoresis of the total 16S rRNA gene pool, isolation and identification of strains, and biodegradation assays. Three strains, Variovorax sp. strain WDL1, Delftia acidovorans WDL34, and Pseudomonas sp. strain WDL5, were isolated directly from the linuron-degrading culture. In addition, subculture of this enrichment culture on potential intermediates in the degradation pathway of linuron (i.e., N,O-dimethylhydroxylamine and 3-chloroaniline) resulted in the isolation of, respectively, Hyphomicrobium sulfonivorans WDL6 and Comamonas testosteroni WDL7. Of these five strains, only Variovorax sp. strain WDL1 was able to use linuron as the sole source of C, N, and energy. WDL1 first converted linuron to 3,4-dichloroaniline (3,4-DCA), which transiently accumulated in the medium but was subsequently degraded. To the best of our knowledge, this is the first report of a strain that degrades linuron further than the aromatic intermediates. Interestingly, the rate of linuron degradation by strain WDL1 was lower than that for the consortium, but was clearly increased when WDL1 was coinoculated with each of the other four strains. D. acidovorans WDL34 and C. testosteroni WDL7 were found to be responsible for degradation of the intermediate 3,4-DCA, and H. sulfonivorans WDL6 was the only strain able to degrade N,O-dimethylhydroxylamine. The role of Pseudomonas sp. strain WDL5 needs to be further elucidated. The degradation of linuron can thus be performed by a single isolate, Variovorax sp. strain WDL1, but is stimulated by a synergistic interaction with the other strains isolated from the same linuron-degrading culture.

Isolation of a bacterial consortium able to degrade the fungicide thiabendazole: the key role of a Sphingomonas phylotype

2017 ◽  
Vol 101 (9) ◽  
pp. 3881-3893 ◽  
Author(s):  
Chiara Perruchon ◽  
Antonis Chatzinotas ◽  
Michalis Omirou ◽  
Sotirios Vasileiadis ◽  
Urania Menkissoglou-Spiroudi ◽  
...  
Keyword(s):  
Bacterial Consortium

scholarly journals Biodecolourisation of Reactive Red 120 as a Sole Carbon Source by a Bacterial Consortium—Toxicity Assessment and Statistical Optimisation

2021 ◽  
Vol 18 (5) ◽  
pp. 2424
Author(s):  
Motharasan Manogaran ◽  
Nur Adeela Yasid ◽  
Ahmad Razi Othman ◽  
Baskaran Gunasekaran ◽  
Mohd Izuan Effendi Halmi ◽  
...  
Keyword(s):  
Carbon Source ◽  
Ammonium Sulphate ◽  
Azo Dye ◽  
Sole Carbon Source ◽  
Bacterial Consortium ◽  
Toxicity Assessment ◽  
Substrate Availability ◽  
Reactive Red 120 ◽  
Significant Attention

The application of microorganisms in azo dye remediation has gained significant attention, leading to various published studies reporting different methods for obtaining the best dye decolouriser. This paper investigates and compares the role of methods and media used in obtaining a bacterial consortium capable of decolourising azo dye as the sole carbon source, which is extremely rare to find. It was demonstrated that a prolonged acclimation under low substrate availability successfully isolated a novel consortium capable of utilising Reactive Red 120 dye as a sole carbon source in aerobic conditions. This consortium, known as JR3, consists of Pseudomonas aeruginosa strain MM01, Enterobacter sp. strain MM05 and Serratia marcescens strain MM06. Decolourised metabolites of consortium JR3 showed an improvement in mung bean’s seed germination and shoot and root length. One-factor-at-time optimisation characterisation showed maximal of 82.9% decolourisation at 0.7 g/L ammonium sulphate, pH 8, 35 °C, and RR120 concentrations of 200 ppm. Decolourisation modelling utilising response surface methodology (RSM) successfully improved decolourisation even more. RSM resulted in maximal decolourisation of 92.79% using 0.645 g/L ammonium sulphate, pH 8.29, 34.5 °C and 200 ppm RR120.

Role of a halothermophilic bacterial consortium for the biodegradation of PAHs and the treatment of petroleum wastewater at extreme conditions

2017 ◽  
Vol 121 ◽  
pp. 44-54 ◽  
Author(s):  
Arulazhagan Pugazhendi ◽  
Huda Qari ◽  
Jalal Mohammad Al-Badry Basahi ◽  
Jean Jacques Godon ◽  
Jeyakumar Dhavamani
Keyword(s):  
Bacterial Consortium ◽  
Extreme Conditions
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