Wheat straw, a potential substrate for cellulase production usingTrichoderma reesei

1993 ◽  
Vol 9 (1) ◽  
pp. 120-121 ◽  
Author(s):  
D. K. Maheswari ◽  
H. Jahan ◽  
J. Paul ◽  
A. Varma
Keyword(s):  
Wheat Straw ◽  
Cellulase Production ◽  
Potential Substrate

Cellulase production by Neurospora crassa on wheat straw

1999 ◽  
Vol 25 (3-5) ◽  
pp. 244-250 ◽  
Author(s):  
M.D Romero ◽  
J Aguado ◽  
L González ◽  
M Ladero
Keyword(s):  
Neurospora Crassa ◽  
Wheat Straw ◽  
Cellulase Production

scholarly journals Production and characterization of cellulase from mushroom (Pleurotus ostreatus) for effective degradation of cellulose

2021 ◽  
Vol 2 (1) ◽  
pp. 135-150
Author(s):  
Beyisa Benti Diro ◽  
Tadessa Daba ◽  
Temam Gemeda Genemo
Keyword(s):  
Solid State ◽  
Wheat Straw ◽  
Solid State Fermentation ◽  
Substrate Concentration ◽  
Pleurotus Ostreatus ◽  
Wood Fiber ◽  
Cellulase Production ◽  
Tree Bark ◽  
Lignocellulosic Substrates ◽  
Optimum Ph

Cellulases are a group of hydrolytic enzymes capable of hydrolyzing the most abundant organic polymer that means cellulose to smaller sugar components including glucose subunits. The aim of this study was to screen cellulase producing oyster mushroom collected from Eucalyptus tree bark to evaluate the in vitro production of cellulase by Pleurotus ostreatus using different lignocellulosic substrates, and to characterize the cellulase produced with respect to changes in pH, temperature, and concentration of substrates. A total of ten mushroom specimens were randomly collected from Eucalyptus tree bark in the premise of Holetta Agricultural Research Center campus. All of the collected mushroom specimens were identified morphologically and biochemically as Pleurotus ostreatus and also screened for their ability to produce cellulase by detecting and measuring zone of hydrolysis on commercial media containing Carbxymethyl Cellulose (CMC) as the sole carbon source. These mushroom specimens were cultivated using both solid state fermentation and submerged fermentation systems supplemented with different lignocellulosic substrates (wheat straw, teff straw, bean straw, wood fiber and Eucalyptus tree bark) to identify the most suitable medium for the production of cellulase. The highest enzyme production was obtained on bean straw and wheat straw which resulted in 0.191 U/ml, 0.868 U/ml and 0.389 U/ml; and 0.216 U/ml, 0.444 U/ml, and 0.245 U/ml of FPase, CMCase, and β-glucosidase in solid state fermentation. The lowest values were, however, obtained in media containing wood fiber in both solid state fermentation and submerged fermentation. Comparison of the lignocellulosic substrates revealed that wheat straw was selected for further growth parameter optimization. The production of cellulase was higher at the 5th day of incubation period, and the optimum pH and incubation temperature required for maximum cellulase production were 4 and 30°C, respectively. Sucrose and Yeast extract at 1% concentration were found to be the most preferred carbon and nitrogen sources for cellulase production by Pleurotus ostreatus. The optimum pH and temperature for cell_free cellulase activity on were found to be 4 and 50°C, respectively. Generally the cellulases produced by Pleurotus ostreatus were stable and active at temperatures ranging from 20-50°C. These characteristics hopefully would make this enzyme potentially attractive in a variety of industrial applications including animal feed treatments. There was a linear relationship between cellulase and its substrate concentration for there was an increase in activity with increase in substrate concentration. The relationship between rate of reaction and substrate concentration depended on the affinity of the enzyme for its substrate. Finally the cellulase was tested for its ability to saccharify agricultural wastes and the results showed the highest release of sugars from wheat straw.

Cellulase Production by Aspergillus fumigatus Cultured on Wheat Straw Waste

2012 ◽  
Vol 11 (3) ◽  
pp. 38-43
Author(s):  
NAZIA MEHBOOB ◽  
JAVAID ASAD ◽  
M Asghar ◽  
M Gulfraz ◽  
Raja Tahir Mahmood
Keyword(s):  
Aspergillus Fumigatus ◽  
Wheat Straw ◽  
Cellulase Production

scholarly journals Lignocelluloytic activities and composition of bacterial community in the camel rumen

2021 ◽  
Vol 7 (3) ◽  
pp. 354-367
Author(s):  
Alaa Emara Rabee ◽  
◽  
Robert Forster ◽  
Ebrahim A Sabra ◽  
◽  
...  
Keyword(s):  
Bacterial Community ◽  
Wheat Straw ◽  
Archaeal Community ◽  
Illumina Miseq ◽  
Cellulase Production ◽  
Poor Quality ◽  
Xylanase Production ◽  
Alfalfa Hay ◽  
Bacterial And Archaeal Communities ◽  
Fiber Fermentation

<abstract> <p>The camel is well-adapted to utilize the poor-quality forages in the harsh desert conditions as the camel rumen sustains fibrolytic microorganisms, mainly bacteria that are capable of breaking down the lignocellulosic biomass efficiently. Exploring the composition of the bacterial community in the rumen of the camel and quantifying their cellulolytic and xylanolytic activities could lead to understanding and improving fiber fermentation and discovering novel sources of cellulases and xylanases. In this study, Illumina MiSeq sequencing of the V4 region on 16S rRNA was applied to identify the bacterial and archaeal communities in the rumen of three camels fed wheat straw and broom corn. Furthermore, rumen samples were inoculated into bacterial media enriched with xylan and different cellulose sources, including filter paper (FP), wheat straw (WS), and alfalfa hay (AH) to assess the ability of rumen bacteria to produce endo-cellulase and endo-xylanase at different fermentation intervals. The results revealed that the phylum Bacteroidetes dominated the bacterial community and <italic>Candidatus Methanomethylophilus</italic> dominated the archaeal community. Also, most of the bacterial community has fibrolytic potential and the dominant bacterial genera were <italic>Prevotella</italic>, <italic>RC9_gut_group</italic>, <italic>Butyrivibrio</italic>, <italic>Ruminococcus</italic>, <italic>Fibrobacteres</italic>, and <italic>Treponema</italic>. The highest xylanase production (884.8 mU/mL) was observed at 7 days. The highest cellulase production (1049.5 mU/mL) was observed when rumen samples were incubated with Alfalfa hay for 7 days.</p> </abstract>

Cellulase production using different streams of wheat grain- and wheat straw-based ethanol processes

2010 ◽  
Vol 38 (7) ◽  
pp. 791-802 ◽  
Author(s):  
Miklós Gyalai-Korpos ◽  
Réka Mangel ◽  
Pablo Alvira ◽  
Dóra Dienes ◽  
Mercedes Ballesteros ◽  
...  
Keyword(s):  
Wheat Straw ◽  
Cellulase Production ◽  
Wheat Grain

Enhanced cellulase production from Trichoderma reesei QM 9414 on physically treated wheat straw

1986 ◽  
Vol 24 (3) ◽  
pp. 218-223 ◽  
Author(s):  
C. Acebal ◽  
M. P. Castillon ◽  
P. Estrada ◽  
I. Mata ◽  
E. Costa ◽  
...  
Keyword(s):  
Wheat Straw ◽  
Trichoderma Reesei ◽  
Cellulase Production
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