Enhanced cellulase production from Trichoderma reesei QM 9414 on physically treated wheat straw

1986 ◽  
Vol 24 (3) ◽  
pp. 218-223 ◽  
Author(s):  
C. Acebal ◽  
M. P. Castillon ◽  
P. Estrada ◽  
I. Mata ◽  
E. Costa ◽  
...  
Keyword(s):  
Wheat Straw ◽  
Trichoderma Reesei ◽  
Cellulase Production

Cellulase production by Trichoderma reesei on wheat straw

1985 ◽  
Vol 13 (2) ◽  
pp. 453-455 ◽  
Author(s):  
C. ACEBAL ◽  
M. P. CASTILLON ◽  
P. ESTRADA ◽  
I. MATA ◽  
E. COSTA ◽  
...  
Keyword(s):  
Wheat Straw ◽  
Trichoderma Reesei ◽  
Cellulase Production

Cellulase production and morphology of Trichoderma reesei in different experimental conditions

2018 ◽  
Vol 63 (2) ◽  
pp. 115-129
Author(s):  
Rahela Carpa ◽  
◽  
Alin Cândea ◽  
Alexei Remizovschi ◽  
Lucian Barbu-Tudoran ◽  
...  
Keyword(s):  
Trichoderma Reesei ◽  
Cellulase Production ◽  
Experimental Conditions

scholarly journals cAMP activates calcium signalling via phospholipase C to regulate cellulase production in the filamentous fungus Trichoderma reesei

2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Yumeng Chen ◽  
Xingjia Fan ◽  
Xinqing Zhao ◽  
Yaling Shen ◽  
Xiangyang Xu ◽  
...  
Keyword(s):  
Signal Transduction ◽  
Phospholipase C ◽  
Trichoderma Reesei ◽  
Filamentous Fungus ◽  
Calcium Signalling ◽  
Signal Transduction Pathway ◽  
Cellulase Production ◽  
Signalling Pathway ◽  
Dependent Manner ◽  
Transduction Pathway

Abstract Background The filamentous fungus Trichoderma reesei is one of the best producers of cellulase and has been widely studied for the production of cellulosic ethanol and bio-based products. We previously reported that Mn2+ and N,N-dimethylformamide (DMF) can stimulate cellulase overexpression via Ca2+ bursts and calcium signalling in T. reesei under cellulase-inducing conditions. To further understand the regulatory networks involved in cellulase overexpression in T. reesei, we characterised the Mn2+/DMF-induced calcium signalling pathway involved in the stimulation of cellulase overexpression. Results We found that Mn2+/DMF stimulation significantly increased the intracellular levels of cAMP in an adenylate cyclase (ACY1)-dependent manner. Deletion of acy1 confirmed that cAMP is crucial for the Mn2+/DMF-stimulated cellulase overexpression in T. reesei. We further revealed that cAMP elevation induces a cytosolic Ca2+ burst, thereby initiating the Ca2+ signal transduction pathway in T. reesei, and that cAMP signalling causes the Ca2+ signalling pathway to regulate cellulase production in T. reesei. Furthermore, using a phospholipase C encoding gene plc-e deletion strain, we showed that the plc-e gene is vital for cellulase overexpression in response to stimulation by both Mn2+ and DMF, and that cAMP induces a Ca2+ burst through PLC-E. Conclusions The findings of this study reveal the presence of a signal transduction pathway in which Mn2+/DMF stimulation produces cAMP. Increase in the levels of cAMP activates the calcium signalling pathway via phospholipase C to regulate cellulase overexpression under cellulase-inducing conditions. These findings provide insights into the molecular mechanism of the cAMP–PLC–calcium signalling pathway underlying cellulase expression in T. reesei and highlight the potential applications of signal transduction in the regulation of gene expression in fungi.

The influence of culture conditions on mycelial structure and cellulase production by Trichoderma reesei Rut C-30

2000 ◽  
Vol 26 (5-6) ◽  
pp. 394-401 ◽  
Author(s):  
F.C. Domingues ◽  
J.A. Queiroz ◽  
J.M.S. Cabral ◽  
L.P. Fonseca
Keyword(s):  
Trichoderma Reesei ◽  
Cellulase Production ◽  
Culture Conditions ◽  
C 30

Cellulase production by Trichoderma reesei immobilized on ?-carrageenan

1982 ◽  
Vol 4 (5) ◽  
pp. 287-292 ◽  
Author(s):  
E. M. Frein ◽  
B. S. Montenecourt ◽  
D. E. Eveleigh
Keyword(s):  
Trichoderma Reesei ◽  
Cellulase Production

The Influence of PH and Dissolved Oxygen Tension (DOT) on Mycelium Growth and Cellulase Production by Trichoderma Reesei

2011 ◽  
Vol 236-238 ◽  
pp. 1005-1013
Author(s):  
Zhi Xi Hang ◽  
Qing Long Rao ◽  
Shi Yuan Yu
Keyword(s):  
Dissolved Oxygen ◽  
Trichoderma Reesei ◽  
Oxygen Tension ◽  
Filter Paper ◽  
Mass Concentration ◽  
Cellulase Production ◽  
Mycelium Growth ◽  
Dissolved Oxygen Tension ◽  
Influence Of Ph ◽  
Filter Paper Activity

The influence of pH and dissolved oxygen tension (DOT) on mycelium growth and cellulase production by Trichoderma reesei was studied in this paper. The experiments were carried out with a cellulose of 10 g/l in a 10 L steam sterilizable bioreactor. The results have shown that H+ concentration was highly fluctuated in the growing and metabolizing periods of mycelium, which went against mycelium growth and cellulase production. Controlling pH to 4.8 was favorable to mycelium growth and cellulase production; the maximum mycelium mass concentration was increased from 2.60 g/l to 2.77 g/l; the maximum filter paper activity was raised from 1.87 IU/ml to 2.79 IU/ml. Meanwhile, the growth and metabolism of mycelium demand an appropriate dissolved oxygen tension (DOT). When the velocity of aeration was increased from 0.4 to 0.5vvm to improve the condition of dissolving oxygen, the mycelium mass concentration was increased from 2.77 g/l to 2.98g/l, and the filter paper activity was raised from 2.79 IU/ml to 2.98 IU/ml.

Proteolytic analysis of Trichoderma reesei in celluase-inducing condition reveals a role for trichodermapepsin (TrAsP) in cellulase production

2019 ◽  
Vol 46 (6) ◽  
pp. 831-842 ◽  
Author(s):  
Nayani Dhanushka Daranagama ◽  
Koki Shioya ◽  
Masahiro Yuki ◽  
Haruna Sato ◽  
Yuki Ohtaki ◽  
...  
Keyword(s):  
Trichoderma Reesei ◽  
Cellulase Production
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