Spectrophotometric determination of carbon monoxide

1977 ◽  
Vol 287 (4-5) ◽  
pp. 313-314 ◽  
Author(s):  
T. V. Ramakrishna ◽  
N. Balasubramanian
Keyword(s):  
Carbon Monoxide ◽  
Spectrophotometric Determination

scholarly journals THE SPECTROPHOTOMETRIC DETERMINATION OF CARBON MONOXIDE

1950 ◽  
Vol 183 (1) ◽  
pp. 297-303
Author(s):  
Niels C. Klendshoj ◽  
Milton. Feldstein ◽  
Alice L. Sprague
Keyword(s):  
Carbon Monoxide ◽  
Spectrophotometric Determination

The Spectrophotometric Determination of Carbon Monoxide in Blood

1963 ◽  
pp. 31-38 ◽  
Author(s):  
JOSEPH S. AMENTA ◽  
R.P. MCDONALD ◽  
A. HAINLINE ◽  
D. MCKAY
Keyword(s):  
Carbon Monoxide ◽  
Spectrophotometric Determination

Methemoglobin in blood as determined by double-wavelength spectrophotometry.

1982 ◽  
Vol 28 (3) ◽  
pp. 508-511 ◽  
Author(s):  
M Sakata ◽  
A Yoshida ◽  
M Haga
Keyword(s):  
Carbon Monoxide ◽  
Spectrophotometric Determination ◽  
Potassium Ferricyanide ◽  
Hemoglobin Derivatives ◽  
Theoretical Results

Abstract We describe spectrophotometric determination of methemoglobin (Met-Hb) in blood by absorbance differences at two wavelengths (characteristic of the instrument used) in the 500-600 nm region. Blood is diluted 100- to 200-fold with a solution containing KCN and carbon monoxide to convert hemoglobin to carboxyhemoglobin and Met-Hb to cyanomethemoglobin (CN-Met-Hb). Carboxyhemoglobin has the same absorbance at the two wavelengths used, so that the differences in absorbance reflect only the CN-Met-Hb component. After measurement of absorbance differences, potassium ferricyanide is added to convert all hemoglobin derivatives to CN-Met-Hb; samples are then remeasured at the same wavelengths. The percentage of Met-Hb is determined from the ratio of the absorbance differences. Results agree satisfactorily with currently accepted procedures and with calculated theoretical results for mixtures of oxyhemoglobin, carboxyhemoglobin, and Met-Hb.

A Modification of the Spectrophotometric Determination of Carbon Monoxide in Blood Enabling Direct Determination of Per Cent Hemoglobin Saturation

1959 ◽  
Vol 5 (2) ◽  
pp. 127-134 ◽  
Author(s):  
Hans Berninger ◽  
Roger Smith
Keyword(s):  
Carbon Monoxide ◽  
Spectrophotometric Determination ◽  
Spectrophotometric Method ◽  
Experimental Error ◽  
Direct Determination ◽  
Hemoglobin Saturation

Abstract A modification of the spectrophotometric method for the determination of carbon monoxide in blood has been presented that enables direct determination of per cent hemoglobin saturation with an overall experimental error of 3 per cent.

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