The mitotic activity of regenerating liver of female mice in different stages of the estrous cycle

1964 ◽  
Vol 51 (6) ◽  
pp. 142-143 ◽  
Author(s):  
C. Bord�n ◽  
J. M. Echave Llanos
Keyword(s):  
Mitotic Activity ◽  
Estrous Cycle ◽  
Regenerating Liver ◽  
Female Mice

Suppression of estrous cycle of female mice by ovariectomized females

1983 ◽  
Vol 17 (2) ◽  
pp. 233-236 ◽  
Author(s):  
A. Marchlewska-Koj ◽  
M. Kruczek ◽  
E. Toch
Keyword(s):  
Estrous Cycle ◽  
Female Mice

AN IMPROVED METHOD FOR CHROMOSOME PREPARATION FROM MOUSE LIVER

1971 ◽  
Vol 13 (3) ◽  
pp. 612-617 ◽  
Author(s):  
N. C. Sun ◽  
E. H. Y. Chu
Keyword(s):  
Mitotic Activity ◽  
Subcutaneous Injection ◽  
Mouse Liver ◽  
Adult Mouse ◽  
Regenerating Liver ◽  
Improved Method ◽  
Mitotic Chromosomes ◽  
Simple Method ◽  
Cytogenetic Analyses ◽  
Adult Mouse Liver

A simple method for preparing mitotic chromosomes from adult mouse liver is described. The procedure involves the accumulation of metaphases in regenerating liver resulting from CCI4 treatment, followed by Trypsin perfusion, hypotonic pretreatment and fixation of cells, and flame-drying of slides to spread chromosomes. Approximately 2 × 106 intact liver cells can be obtained from a single mouse liver – enough to prepare 50 slides. A peak mitotic activity, with more than 1% of cells in mitosis, was observed 72 hr after subcutaneous injection of 0.1 ml of 45% CCI4 per animal. The distribution of diploid, tetraploid, and octaploid cells in mitosis was about 86, 11, and 3%, respectively. The abundant number of analyzable metaphases in such preparations makes this method valuable for cytogenetic analyses of normally non-proliferating tissue from adult laboratory mammals.

scholarly journals Type 1 5α-reductase may be required for estrous cycle changes in affective behaviors of female mice

2012 ◽  
Vol 226 (2) ◽  
pp. 376-380 ◽  
Author(s):  
Carolyn J. Koonce ◽  
Alicia A. Walf ◽  
Cheryl A. Frye
Keyword(s):  
Estrous Cycle ◽  
Female Mice ◽  
Affective Behaviors

Cardiac contraction, calcium transients, and myofilament calcium sensitivity fluctuate with the estrous cycle in young adult female mice

2014 ◽  
Vol 306 (7) ◽  
pp. H938-H953 ◽  
Author(s):  
Jennifer K. MacDonald ◽  
W. Glen Pyle ◽  
Cristine J. Reitz ◽  
Susan E. Howlett
Keyword(s):  
Estrous Cycle ◽  
Action Potentials ◽  
Ventricular Myocytes ◽  
Fold Increase ◽  
Reproductive Hormones ◽  
Cardiac Contraction ◽  
Estrous Cycles ◽  
Female Mice ◽  
Ec Coupling ◽  
The Impact

This study established conditions to induce regular estrous cycles in female C57BL/6J mice and investigated the impact of the estrous cycle on contractions, Ca2+ transients, and underlying cardiac excitation-contraction (EC)-coupling mechanisms. Daily vaginal smears from group-housed virgin female mice were stained to distinguish estrous stage (proestrus, estrus, metestrus, diestrus). Ventricular myocytes were isolated from anesthetized mice. Contractions and Ca2+ transients were measured simultaneously (4 Hz, 37°C). Interestingly, mice did not exhibit regular cycles unless they were exposed to male pheromones in bedding added to their cages. Field-stimulated myocytes from mice in estrus had larger contractions (∼2-fold increase), larger Ca2+ transients (∼1.11-fold increase), and longer action potentials (>2-fold increase) compared with other stages. Larger contractions and Ca2+ transients were not observed in estrus myocytes voltage-clamped with shorter action potentials. Voltage-clamp experiments also demonstrated that estrous stage had no effect on Ca2+ current, EC-coupling gain, diastolic Ca2+, sarcoplasmic reticulum (SR) Ca2+ content, or fractional release. Although contractions were largest in estrus, myofilament Ca2+ sensitivity was lowest (EC50 values ∼1.15-fold higher) in conjunction with increased phosphorylation of myosin binding protein C in estrus. Contractions were enhanced in ventricular myocytes from mice in estrus because action potential prolongation increased SR Ca2+ release. These findings demonstrate that cyclical changes in reproductive hormones associated with the estrous cycle can influence myocardial electrical and contractile function and modify Ca2+ homeostasis. However, such changes are unlikely to occur in female mice housed in groups under conventional conditions, since these mice do not exhibit regular estrous cycles.

scholarly journals THE INCORPORATION OF FORMATE-C14 INTO THE NUCLEIC ACIDS OF RATS WITH REGENERATING LIVER AND NOVIKOFF HEPATOMA

1959 ◽  
Vol 37 (1) ◽  
pp. 1405-1416 ◽  
Author(s):  
J. C. Nixon ◽  
S. H. Zbarsky
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Mitotic Activity ◽  
Intestinal Mucosa ◽  
Regenerating Liver ◽  
Simultaneous Presence ◽  
Novikoff Hepatoma ◽  
Mitotic Frequency ◽  
Host Tissues

A study was made of the incorporation in vivo of formate-C14 into the purines and thymine of regenerating liver and Novikoff hepatoma in the rat, during the period of maximum mitotic activity of these tissues. The effects of these tissues on one another and on certain host tissues were also studied. The maximum mitotic frequency of Novikoff hepatoma was observed on the 4th day of growth following transplantation. This tumor caused a decrease in formate incorporation into the nucleic acid purines and thymine of the host's spleen and intestinal mucosa but had little effect on liver. The results also indicated that the uptake of formate by the RNA adenine of spleen and intestinal mucosa and the DNA thymine of intestinal mucosa was diminished by the presence of regenerating liver. The simultaneous presence of both regenerating liver and Novikoff hepatoma generally lowered the incorporation of formate-C14 into the nucleic acids of the host spleen and intestinal mucosa. It was observed further that the utilization of formate by the nucleic acids of Novikoff hepatoma and regenerating rat liver was decreased in animals containing both of these rapidly dividing tissues.

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