Effect of an excess of thyroid hormones in rabbits on mitotic activity of the regenerating liver cells

1972 ◽  
Vol 74 (3) ◽  
pp. 1089-1091
Author(s):  
E. F. Panachin ◽  
L. E. Nemirovskii ◽  
V. V. Klimenko
Keyword(s):  
Thyroid Hormones ◽  
Mitotic Activity ◽  
Liver Cells ◽  
Regenerating Liver

The endoplasmic reticulum in regenerating liver cells

1985 ◽  
Vol 240 (1) ◽  
Author(s):  
Lillemor Lewan ◽  
Hadar Emanuelsson
Keyword(s):  
Endoplasmic Reticulum ◽  
Liver Cells ◽  
Regenerating Liver

AN IMPROVED METHOD FOR CHROMOSOME PREPARATION FROM MOUSE LIVER

1971 ◽  
Vol 13 (3) ◽  
pp. 612-617 ◽  
Author(s):  
N. C. Sun ◽  
E. H. Y. Chu
Keyword(s):  
Mitotic Activity ◽  
Subcutaneous Injection ◽  
Mouse Liver ◽  
Adult Mouse ◽  
Regenerating Liver ◽  
Improved Method ◽  
Mitotic Chromosomes ◽  
Simple Method ◽  
Cytogenetic Analyses ◽  
Adult Mouse Liver

A simple method for preparing mitotic chromosomes from adult mouse liver is described. The procedure involves the accumulation of metaphases in regenerating liver resulting from CCI4 treatment, followed by Trypsin perfusion, hypotonic pretreatment and fixation of cells, and flame-drying of slides to spread chromosomes. Approximately 2 × 106 intact liver cells can be obtained from a single mouse liver – enough to prepare 50 slides. A peak mitotic activity, with more than 1% of cells in mitosis, was observed 72 hr after subcutaneous injection of 0.1 ml of 45% CCI4 per animal. The distribution of diploid, tetraploid, and octaploid cells in mitosis was about 86, 11, and 3%, respectively. The abundant number of analyzable metaphases in such preparations makes this method valuable for cytogenetic analyses of normally non-proliferating tissue from adult laboratory mammals.

1α, 25-Dihydroxyvitamin D3 enables regenerating liver cells to make functional ribonucleotide reductase subunits and replicate DNA in thyroparathyroidectomized rats

1985 ◽  
Vol 63 (5) ◽  
pp. 319-324 ◽  
Author(s):  
T. Youdale ◽  
J. F. Whitfield ◽  
R. H. Rixon
Keyword(s):  
Body Weight ◽  
Dna Replication ◽  
Ribonucleotide Reductase ◽  
Intraperitoneal Injection ◽  
Liver Cells ◽  
Regenerating Liver ◽  
Dihydroxyvitamin D3 ◽  
Dihydroxyvitamin D ◽  
Do So

Between 16 and 20 h after partial (70%) hepatectomy (HPX) in normal rats, the remaining liver cells accumulate ribonucleotide reductase subunits, assemble these into active holoenzyme, and initiate DNA replication. These late prereplicative activities did not occur in most of the liver cells remaining after HPX in rats which had been thyroparathyroidectomized (TPTX) 72 h previously. However, one intraperitoneal injection of 400 or 600 ng 1α, 25-dihydroxyvitamin D3/100 g body weight at the time of HPX enabled the remaining liver cells in such TPTX rats to make functional ribonucleotide reductase subunits, assemble these subunits into active CDP-reducing holoenzymes, and replicate their DNA, though they started to do so 4 to 16 h later than in normal animals.

Uptake of Protein by Regenerating Liver Cells

Nature ◽  
1964 ◽  
Vol 204 (4964) ◽  
pp. 1210-1211 ◽  
Author(s):  
T. GHOSE ◽  
S. C. TSO
Keyword(s):  
Liver Cells ◽  
Regenerating Liver

The gene encoding rat insulinlike growth factor-binding protein 1 is rapidly and highly induced in regenerating liver

1991 ◽  
Vol 11 (3) ◽  
pp. 1393-1401 ◽  
Author(s):  
K L Mohn ◽  
A E Melby ◽  
D S Tewari ◽  
T M Laz ◽  
R Taub
Keyword(s):  
Growth Factor ◽  
Binding Protein ◽  
Normal Liver ◽  
Liver Cells ◽  
Immediate Early ◽  
Cdna Libraries ◽  
Regenerating Liver ◽  
Liver Growth ◽  
Insulinlike Growth Factor ◽  
Igf I

The liver is an epithelioid organ that can regenerate following partial hepatectomy. Although it is composed mainly of hepatocytes, it has a complex, multicellular architecture, implying that intercellular communications must exist during regeneration. As in other mitogen-stimulated cells, immediate-early growth response genes induced in the absence of prior protein synthesis are likely to play an important regulatory role in the regenerative process. Through differential screening of regenerating liver cDNA libraries, we found that one of the most highly expressed immediate-early genes in liver regeneration encodes the rat homolog of the low-molecular-weight insulinlike growth factor (IGF)-binding protein (IGFBP-1). This protein has been implicated in enhancing the mitogenic effect of IGF on tissues. IGFBP-1 gene induction is transcriptionally mediated and specific to regenerating liver, as the gene is not expressed in mitogen-stimulated fibroblasts. IGFBP-1 expression has been shown to increase under low-insulin conditions such as diabetes, and the complex regulation of expression is indicated by our finding that insulin treatment of H35 rat hepatoma cells, which induces proliferation, also causes a rapid decrease in transcription and expression of the IGFBP-1 gene. Of note, IGFBP-1 mRNA is abundant in fetal rat liver, implying that it participates in normal liver growth and development. Although regenerating liver cells continue to produce IGF-I, we did not detect IGF-I receptor mRNA during the first 24 h after hepatectomy. However, some IGFBPs may act to enhance the activity of IGF-I independently of IGF-I receptors. Thus, IGF-1 and IGFBPs may interact with hepatocytes or nonparenchymal liver cells, through either IGF-I or novel receptors. In this way, IGFBP-I and IGF-I could act in a paracrine and/or autocrine fashion in maintaining normal liver architecture during regeneration.

scholarly journals THE INCORPORATION OF FORMATE-C14 INTO THE NUCLEIC ACIDS OF RATS WITH REGENERATING LIVER AND NOVIKOFF HEPATOMA

1959 ◽  
Vol 37 (1) ◽  
pp. 1405-1416 ◽  
Author(s):  
J. C. Nixon ◽  
S. H. Zbarsky
Keyword(s):  
Nucleic Acid ◽  
Nucleic Acids ◽  
Mitotic Activity ◽  
Intestinal Mucosa ◽  
Regenerating Liver ◽  
Simultaneous Presence ◽  
Novikoff Hepatoma ◽  
Mitotic Frequency ◽  
Host Tissues

A study was made of the incorporation in vivo of formate-C14 into the purines and thymine of regenerating liver and Novikoff hepatoma in the rat, during the period of maximum mitotic activity of these tissues. The effects of these tissues on one another and on certain host tissues were also studied. The maximum mitotic frequency of Novikoff hepatoma was observed on the 4th day of growth following transplantation. This tumor caused a decrease in formate incorporation into the nucleic acid purines and thymine of the host's spleen and intestinal mucosa but had little effect on liver. The results also indicated that the uptake of formate by the RNA adenine of spleen and intestinal mucosa and the DNA thymine of intestinal mucosa was diminished by the presence of regenerating liver. The simultaneous presence of both regenerating liver and Novikoff hepatoma generally lowered the incorporation of formate-C14 into the nucleic acids of the host spleen and intestinal mucosa. It was observed further that the utilization of formate by the nucleic acids of Novikoff hepatoma and regenerating rat liver was decreased in animals containing both of these rapidly dividing tissues.

Intracellular ribonucleic acid composition in regenerating liver cells

1958 ◽  
Vol 27 ◽  
pp. 395-401 ◽  
Author(s):  
Gaston de Lamirande ◽  
Claude Allard ◽  
Antonio Cantero
Keyword(s):  
Acid Composition ◽  
Ribonucleic Acid ◽  
Liver Cells ◽  
Regenerating Liver
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