Maximum degree of fluorescence polarization for two-photon-excited complex molecules

1972 ◽  
Vol 17 (3) ◽  
pp. 1138-1140
Author(s):  
E. S. Voropai ◽  
I. I. Zholnerevich ◽  
A. M. Sarzhevskii
Keyword(s):  
Fluorescence Polarization ◽  
Maximum Degree ◽  
Complex Molecules ◽  
Two Photon ◽  
Excited Complex

Investigation of the polarization and absorption spectra of complex molecules with two-photon excitation

1975 ◽  
Vol 23 (2) ◽  
pp. 1079-1082
Author(s):  
E. S. Voropai ◽  
I. A. Dudarev ◽  
A. P. Klishchenko ◽  
A. M. Sarzhevskii
Keyword(s):  
Absorption Spectra ◽  
Complex Molecules ◽  
Two Photon ◽  
Photon Excitation ◽  
Two Photon Excitation

Fluorescence of Excited Complex Molecules in Mercury Photosensitization

1966 ◽  
Vol 45 (6) ◽  
pp. 2322-2323 ◽  
Author(s):  
S. Penzes ◽  
O. P. Strausz ◽  
H. E. Gunning
Keyword(s):  
Complex Molecules ◽  
Excited Complex

Extending the Reach of Immunoassays to Optically Dense Specimens by Using Two-Photon Excited Fluorescence Polarization

2000 ◽  
Vol 72 (22) ◽  
pp. 5748-5752 ◽  
Author(s):  
Gary A. Baker ◽  
Siddharth Pandey ◽  
Frank V. Bright
Keyword(s):  
Fluorescence Polarization ◽  
Two Photon

scholarly journals INFLUENCE OF DIPOLE-DIPOLE INTERACTION ON DYNAMICS OF ENTANGLED GREENBERGER — HORNE — ZEILINGER STATES FOR TWO QUBITS

2017 ◽  
Vol 19 (9.1) ◽  
pp. 112-121
Author(s):  
E.K. Bashkirov ◽  
T.A. Puzyrnaya
Keyword(s):  
Sudden Death ◽  
Maximum Degree ◽  
Dipole Interaction ◽  
Entanglement Sudden Death ◽  
Ghz States ◽  
Two Photon ◽  
Degree Of Entanglement

The influence of dipole-dipole interaction on dynamics of three- and four-particle GHZ states for two Д-type superconducting flux cubits interacting with one or two different electronic LC cavities via two-photon processes has been investigated. The results show that dipole-dipole interaction does not lead to the disappearance of the effect of entanglement sudden death and does not change the maximum degree of entanglement.

Two-Photon Fluorescence Polarization Anisotropy Decay on Highly Diluted Solutions by Phase Fluorometry

2001 ◽  
Vol 55 (3) ◽  
pp. 311-317 ◽  
Author(s):  
Francesca Olivini ◽  
Sabrina Beretta ◽  
Giuseppe Chirico
Keyword(s):  
Fluorescence Polarization ◽  
Light Polarization ◽  
Correlation Spectroscopy ◽  
Polarization Anisotropy ◽  
Two Photon ◽  
Protein Size ◽  
High Dilutions ◽  
Fluorescence Polarization Anisotropy ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

We report here the phase-sensitive measurements of two-photon fluorescence polarization anisotropy (FPA) on highly diluted solutions. We first describe the characterization of the response of the two-photon microscope to the light polarization and its test by means of measurements of the FPA on rhodamine 6G as a function of the viscosity. Further, we report the study of the FPA at high dilutions on a globular protein, the beta-lactoglobulin B (BLG) labeled with Alexa 532. The number of molecules (from 0.4 to 17 molecules per excitation volume) is measured by means of fluorescence correlation spectroscopy (FCS). The average rotational and translational diffusion coefficients measured with the FPA and FCS methods are in good agreement with the protein size and do not show a substantial dependence on the protein concentration, despite the very low signal (≌3 times the background) observed for highly diluted solutions.

Possible advantages of the excitation of stimulated two-photon emission in complex molecules

1985 ◽  
Vol 28 (12) ◽  
pp. 1069-1075
Author(s):  
N. P. Bashlakova ◽  
V. R. Blok ◽  
Yu. G. Khronopulo
Keyword(s):  
Photon Emission ◽  
Complex Molecules ◽  
Two Photon

scholarly journals Two-Photon Excitation in Fluorescence Polarization Receptor-Ligand Binding Assay

2005 ◽  
Vol 10 (4) ◽  
pp. 314-319 ◽  
Author(s):  
Marko E. Tirri ◽  
Roope J. Huttunen ◽  
Juha Toivonen ◽  
Pirkko L. Härkönen ◽  
Juhani T. Soini ◽  
...  
Keyword(s):  
Ligand Binding ◽  
Fluorescence Polarization ◽  
Binding Assay ◽  
Ligand Binding Assay ◽  
Receptor Ligand ◽  
Lead Compounds ◽  
Two Photon ◽  
Photon Excitation ◽  
Homogeneous Assay ◽  
Estrogen Receptor Ligand

Fluorescence polarization is one of the most commonly used homogeneous assay principles in drug discovery for screening of potential lead compounds. In this article, the fluorescence polarization technique is combined with 2-photon excitation of fluorescence. Theoretically, the use of 2-photon excitation of fluorescence increases the volumetric sensitivity and polarization contrast of fluorescence polarization assays. The work in this report demonstrates these predictions for an estrogen receptor ligand binding assay.

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