scholarly journals A molecular theory for two-photon and three-photon fluorescence polarization

2011 ◽  
Vol 134 (9) ◽  
pp. 094503 ◽  
Author(s):  
J. M. Leeder ◽  
D. L. Andrews
Keyword(s):  
Fluorescence Polarization ◽  
Molecular Theory ◽  
Two Photon ◽  
Photon Fluorescence

Two-Photon Fluorescence Polarization Anisotropy Decay on Highly Diluted Solutions by Phase Fluorometry

2001 ◽  
Vol 55 (3) ◽  
pp. 311-317 ◽  
Author(s):  
Francesca Olivini ◽  
Sabrina Beretta ◽  
Giuseppe Chirico
Keyword(s):  
Fluorescence Polarization ◽  
Light Polarization ◽  
Correlation Spectroscopy ◽  
Polarization Anisotropy ◽  
Two Photon ◽  
Protein Size ◽  
High Dilutions ◽  
Fluorescence Polarization Anisotropy ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

We report here the phase-sensitive measurements of two-photon fluorescence polarization anisotropy (FPA) on highly diluted solutions. We first describe the characterization of the response of the two-photon microscope to the light polarization and its test by means of measurements of the FPA on rhodamine 6G as a function of the viscosity. Further, we report the study of the FPA at high dilutions on a globular protein, the beta-lactoglobulin B (BLG) labeled with Alexa 532. The number of molecules (from 0.4 to 17 molecules per excitation volume) is measured by means of fluorescence correlation spectroscopy (FCS). The average rotational and translational diffusion coefficients measured with the FPA and FCS methods are in good agreement with the protein size and do not show a substantial dependence on the protein concentration, despite the very low signal (≌3 times the background) observed for highly diluted solutions.

Lysosomal targeted NIR photosensitizer for photodynamic therapy and two-photon fluorescent imaging

2021 ◽  
Author(s):  
Ruiyuan Liu ◽  
Yuping Zhou ◽  
Di Zhang ◽  
Genghan He ◽  
Chuang Liu ◽  
...  
Keyword(s):  
Photodynamic Therapy ◽  
Fluorescence Imaging ◽  
Near Infrared ◽  
Fluorescent Imaging ◽  
High Fidelity ◽  
Design And Synthesis ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Design and synthesis of near-infrared (NIR) emissive fluorophore for imaging of organelle and photodynamic therapy has received enormous attention. Hence, NIR emissive fluorophore of high-fidelity lysosome targeting, two-photon fluorescence imaging,...

scholarly journals Molecular design strategy to alleviate environmental interference on two-photon fluorescence probes

2021 ◽  
Vol 2 (6) ◽  
pp. 100471
Author(s):  
Xingxing Zhang ◽  
Tianbing Ren ◽  
Qianling Zhang ◽  
Ting Zeng ◽  
Zhe Li ◽  
...  
Keyword(s):  
Molecular Design ◽  
Design Strategy ◽  
Fluorescence Probes ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence ◽  
Environmental Interference

scholarly journals High-speed label-free two-photon fluorescence microscopy of metabolic transients during neuronal activity

2021 ◽  
Vol 118 (8) ◽  
pp. 081104
Author(s):  
Andrew J. Bower ◽  
Carlos Renteria ◽  
Joanne Li ◽  
Marina Marjanovic ◽  
Ronit Barkalifa ◽  
...  
Keyword(s):  
Fluorescence Microscopy ◽  
Neuronal Activity ◽  
High Speed ◽  
Label Free ◽  
Two Photon ◽  
Metabolic Transients ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence

Two-photon fluorescence sensors for imaging NMDA receptors and monitoring release of Zn2+ from the presynaptic terminal

2017 ◽  
Vol 91 ◽  
pp. 770-779 ◽  
Author(s):  
Xiaoqiang Chen ◽  
Chang Su Lim ◽  
Dayoung Lee ◽  
Songyi Lee ◽  
Sang Jun Park ◽  
...  
Keyword(s):  
Nmda Receptors ◽  
Presynaptic Terminal ◽  
Fluorescence Sensors ◽  
Two Photon ◽  
Two Photon Fluorescence ◽  
Photon Fluorescence
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