Use of an extraction-photometric method in the quantitative determination of amino sugars for investigating natural polymers

1986 ◽  
Vol 22 (6) ◽  
pp. 718-719
Author(s):  
T. I. Burtseva ◽  
S. A. Cherkasova ◽  
Yu. S. Ovodov
Keyword(s):  
Quantitative Determination ◽  
Photometric Method ◽  
Amino Sugars ◽  
Natural Polymers

scholarly journals Determination of amino sugars in mixtures containing glucosamine, galactosamine and muramic acid

1968 ◽  
Vol 109 (1) ◽  
pp. 13-18 ◽  
Author(s):  
D. E. S. Stewart-Tull
Keyword(s):  
Cell Wall ◽  
Quantitative Determination ◽  
Column Chromatography ◽  
Bacterial Species ◽  
Colorimetric Method ◽  
Previous Treatment ◽  
Amino Sugars ◽  
Muramic Acid ◽  
Molar Ratios

A colorimetric method is described whereby the direct quantitative determination of glucosamine, galactosamine and muramic acid can be achieved without previous treatment of the cell-wall hydrolysate, for example by column chromatography. Molar ratios of hexosamines in cell-wall preparations, from a number of bacterial species, determined by this method were found to be in general agreement with previously published results.

scholarly journals A new method for the quantitative determination of monosaccharides, amino sugars and N-acetylneuraminic acid and of 6-deoxyhexose (fucose) in the presence of other sugars

1968 ◽  
Vol 110 (3) ◽  
pp. 413-417 ◽  
Author(s):  
Gwyneth M. Brearley ◽  
Jacqueline B. Weiss
Keyword(s):  
Quantitative Determination ◽  
Colorimetric Assay ◽  
Periodate Oxidation ◽  
New Method ◽  
Amino Sugars ◽  
Simple Method ◽  
Acetylneuraminic Acid ◽  
Assay Procedure ◽  
Optimum Ph

1. Monosaccharides, amino sugars and N-acetylneuraminic acid were determined by using an original colorimetric assay procedure, based on the detection of formaldehyde released after periodate oxidation. A range of these compounds was investigated by this method and they were all found to obey Beer's law within the concentration range 0–0·6μmole/ml. 2. A simple method for the determination of 6-deoxyhexose concentration in the presence of other monosaccharides is also described. 3. The optimum pH for the release of formaldehyde from sugars by periodate oxidation was 7·0–7·5. 4. The methods described have considerable advantages over existing assay systems and their particlar value in automatic colorimetry, where the use of concentrated acids is undesirable, is discussed.

Extraction photometric method for the quantitative determination of metamizil in 0.25% solution for injection

1980 ◽  
Vol 14 (5) ◽  
pp. 350-353
Author(s):  
A. A. Bagdasaryan ◽  
L. S. Efimova ◽  
S. A. Minina ◽  
A. S. Bril'
Keyword(s):  
Quantitative Determination ◽  
Photometric Method

Quantitative Determination of Vanillin and Ethyl Vanillin in Flavors by Paper Chromatography

1964 ◽  
Vol 47 (6) ◽  
pp. 1161-1165
Author(s):  
J Fitelson
Keyword(s):  
Quantitative Determination ◽  
Paper Chromatography ◽  
Alkaline Solution ◽  
Chromatographic Separation ◽  
Chromatographic Method ◽  
Ultraviolet Absorption ◽  
Photometric Method ◽  
Absorption Method ◽  
Ethyl Vanillin

Abstract Previous methods for estimating vanillin and ethyl vanillin in mixtures were long and complicated. However, this paper presents a simple chromatographic separation and quantitative determination of these aromatics, using Mitchell equipment for paper chromatography and 8 × 8” papers. Development for 2 hours separates the compounds adequately; they are then extracted and measured by absorbances at 348 mμ in alkaline solution. Recoveries of added vanillin and ethyl vanillin to vanilla extract are excellent. Aside from normal manipulative errors, the only significant inaccuracy is caused by the small amount of natural p-hy-droxybenzaldehyde in vanilla (usually equivalent to about 5% of the vanillin content). The method does not separate p-hydroxybenzaldehyde and vanillin. Total errors in the method do not exceed 0.01%. Comparison of the method with the two AOAC methods for vanillin shows that the paper chromatographic method gives results close to those by the ultraviolet absorption method, in most cases, and significantly lower results than the photometric method, which is known to give erroneously high results.

Quantitative determination of longitudinal and transverse cross-relaxation rates

1999 ◽  
Vol 96 (9/10) ◽  
pp. 1608-1615
Author(s):  
T. E. Malliavin ◽  
H. Desvaux ◽  
M. A. Delsuc
Keyword(s):  
Quantitative Determination ◽  
Cross Relaxation ◽  
Relaxation Rates
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