Lack of transport of erythropoietin across the human placenta as studied by an in vitro perfusion system

1994 ◽  
Vol 427 (1-2) ◽  
pp. 157-161 ◽  
Author(s):  
Antoine Malek ◽  
Ruth Sager ◽  
Kai -Uwe Eckardt ◽  
Christian Bauer ◽  
Henning Schneider
Keyword(s):  
Human Placenta ◽  
Perfusion System ◽  
In Vitro Perfusion

Effect of liposomal encapsulation of chloramphenicol on its transfer across the human placenta in a dual in vitro perfusion system

1992 ◽  
Vol 88 (1-3) ◽  
pp. 313-317 ◽  
Author(s):  
M.A. Onur ◽  
C.J. Kirby ◽  
A. Isimer ◽  
S. Beksac ◽  
N. Basci ◽  
...  
Keyword(s):  
Human Placenta ◽  
Perfusion System ◽  
In Vitro Perfusion

Placental transfer of steroids: effect of binding to serum albumin and to placenta

1980 ◽  
Vol 238 (3) ◽  
pp. E208-E213 ◽  
Author(s):  
J. Dancis ◽  
V. Jansen ◽  
M. Levitz
Keyword(s):  
Human Placenta ◽  
Transfer Rate ◽  
Placental Transfer ◽  
Perfusion System ◽  
In Vitro Perfusion ◽  
Fetal Circulation ◽  
Transfer Rates ◽  
Effective Factor ◽  
High Concentrations

The transfer rates and placental retention of a series of steroids were measured using an in vitro perfusion system of an isolated cotyledon of human placenta. The steroids were added to the maternal inflow and rates of appearance in maternal and fetal outflows were measured, from which data were calculated the transfer rate and placental retention. With a low concentration of albumin (0.01 g/dl) in the maternal and fetal perfusates, transfer rates of diethylstilbestrol and ethynylestradiol were initially low, with considerable retention of the steroids within the placenta. Transfer rates increased with duration of perfusion. With high concentrations of albumin (1 g/dl), placental retention was greatly reduced and transfer rates very rapidly reached high levels. Albumin in the fetal circulation was the effective factor in increasing transfer rate; maternal albumin reduced it. The results with estrone and progesterone were qualitatively similar but not as striking, posssibly because of the large endogenous concentrations of these two hormones. Placental retention of dexamethasone, a more polar steroid that does not bind to placenta and binds poorly to albumin, was low and there was little difference between transfer from low- and high-protein perfusates.

Apoprotein C effect on triglyceride transport in tandem-perfused rat hind end and liver

1984 ◽  
Vol 247 (3) ◽  
pp. G305-G310
Author(s):  
W. J. Kortz ◽  
J. R. Nashold ◽  
M. R. Greenfield ◽  
H. Hilderman ◽  
S. H. Quarfordt
Keyword(s):  
Fatty Acid ◽  
Free Fatty Acid ◽  
Fatty Acyl ◽  
Liver Fat ◽  
Molar Ratio ◽  
Perfusion System ◽  
In Vitro Perfusion ◽  
Arterial Inflow ◽  
High Concentrations

The metabolism of double-labeled triglyceride in a synthetic emulsion was defined in an in vitro perfusion system of rat hind end and liver described previously [Am. J. Physiol. 245 (Gastrointest. Liver Physiol. 8): G106-G112, 1983]. The metabolism of [3H]glycerol-[14C]triolein was defined in the absence of added apoproteins and with additions of human CII and both CII and CIII. Without apoprotein, a pronounced lipolysis of the triglyceride was recognized by high concentrations of radiolabeled glycerol and free fatty acid in the perfusate. The removal of an aliquot of hind-end venous effluent 5 min after adding the labeled triglyceride emulsion to the arterial inflow demonstrated a brisk lipolysis of the substrate when incubated outside the perfusion system. The addition of CII protein to the emulsion before its introduction into the tandem system eliminated perfusate lipolysis, both within the perfusion system and in incubations of aliquots withdrawn from the system. Intravascular lipolysis was not seen with triglyceride emulsions containing both CII and CIH or when an aliquot of hind-end venous effluent was incubated with triglycerides that had not been exposed to the perfusion system. The intravascular lipolysis observed for the [14C]triglyceride added to the tandem system without apoproteins was associated with relatively greater recoveries of 14C-fatty acyl in liver, fat, and muscle and relatively greater recoveries of 14CO2 than when CII alone or both CII and CIII were added with the triglyceride. The addition of CIII to CII in a 1:1 molar ratio increased the recovery of 14C-fatty acyl in muscle and the recovery as 14CO2.(ABSTRACT TRUNCATED AT 250 WORDS)

Transfer and Uptake of Alfentanil in the Human Placenta During In Vitro Perfusion

1995 ◽  
Vol 39 (4) ◽  
pp. 235
Author(s):  
M. I. ZAKOWSKI ◽  
A. A. HAM ◽  
G. J GRANT
Keyword(s):  
Human Placenta ◽  
In Vitro Perfusion

In vitro Perfusion Studies of the Human Placenta

1976 ◽  
Vol 7 (6) ◽  
pp. 344-357 ◽  
Author(s):  
Philip A. Rice ◽  
Robert E.L. Nesbitt, Jr. ◽  
James E. Rourke
Keyword(s):  
Human Placenta ◽  
In Vitro Perfusion ◽  
Perfusion Studies

An In Vitro Perfusion System to Examine the Responses of Endothelial Cells to Simulated Flow and Inflammatory Stimulation

2009 ◽  
Vol 37 (6) ◽  
pp. 657-669 ◽  
Author(s):  
Natalia Y. Cockcroft ◽  
Oluwatobiloba Oke ◽  
Fiona Cunningham ◽  
Emma Bishop ◽  
Ian M. Fearon ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Perfusion System ◽  
In Vitro Perfusion ◽  
Simulated Flow

scholarly journals Evaluation of Hydrophilic Polymer Embolization From Endovascular Sheath Devices in an In Vitro Perfusion System

2020 ◽  
Vol 72 (5) ◽  
pp. e351-e352
Author(s):  
A. Mordhorst ◽  
G. Yang ◽  
B.Y. Zhang ◽  
S.S. Suri ◽  
J. Kizhakkedathu ◽  
...  
Keyword(s):  
Hydrophilic Polymer ◽  
Perfusion System ◽  
In Vitro Perfusion
Sign in / Sign up

Export Citation Format

Share Document