Influence of Fenoterol on Glucose Utilization by Human Placental Tissue in an in vitro Perfusion System

The metabolism of double-labeled triglyceride in a synthetic emulsion was defined in an in vitro perfusion system of rat hind end and liver described previously [Am. J. Physiol. 245 (Gastrointest. Liver Physiol. 8): G106-G112, 1983]. The metabolism of [3H]glycerol-[14C]triolein was defined in the absence of added apoproteins and with additions of human CII and both CII and CIII. Without apoprotein, a pronounced lipolysis of the triglyceride was recognized by high concentrations of radiolabeled glycerol and free fatty acid in the perfusate. The removal of an aliquot of hind-end venous effluent 5 min after adding the labeled triglyceride emulsion to the arterial inflow demonstrated a brisk lipolysis of the substrate when incubated outside the perfusion system. The addition of CII protein to the emulsion before its introduction into the tandem system eliminated perfusate lipolysis, both within the perfusion system and in incubations of aliquots withdrawn from the system. Intravascular lipolysis was not seen with triglyceride emulsions containing both CII and CIH or when an aliquot of hind-end venous effluent was incubated with triglycerides that had not been exposed to the perfusion system. The intravascular lipolysis observed for the [14C]triglyceride added to the tandem system without apoproteins was associated with relatively greater recoveries of 14C-fatty acyl in liver, fat, and muscle and relatively greater recoveries of 14CO2 than when CII alone or both CII and CIII were added with the triglyceride. The addition of CIII to CII in a 1:1 molar ratio increased the recovery of 14C-fatty acyl in muscle and the recovery as 14CO2.(ABSTRACT TRUNCATED AT 250 WORDS)

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Synthesis and release of placental proteins by in vitro perfused human placental tissue

Placenta ◽

10.1016/s0143-4004(86)80068-6 ◽

1986 ◽

Vol 7 (5) ◽

pp. 460-461

Author(s):

N.A. Bersinger ◽

H. Schneider

Keyword(s):

Placental Tissue ◽

Placental Proteins ◽

Human Placental Tissue

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Effect of liposomal encapsulation of chloramphenicol on its transfer across the human placenta in a dual in vitro perfusion system

International Journal of Pharmaceutics ◽

10.1016/0378-5173(92)90329-z ◽

1992 ◽

Vol 88 (1-3) ◽

pp. 313-317 ◽

Cited By ~ 3

Author(s):

M.A. Onur ◽

C.J. Kirby ◽

A. Isimer ◽

S. Beksac ◽

N. Basci ◽

...

Keyword(s):

Human Placenta ◽

Perfusion System ◽

In Vitro Perfusion

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EFFECT OF IONIC ENVIRONMENT ON THE RELEASE OF HUMAN PLACENTAL LACTOGEN IN VITRO

Journal of Endocrinology ◽

10.1677/joe.0.0690349 ◽

1976 ◽

Vol 69 (3) ◽

pp. 349-358 ◽

Cited By ~ 16

Author(s):

V. J. CHOY ◽

W. B. WATKINS

Keyword(s):

Placental Tissue ◽

Placental Lactogen ◽

Human Placental Lactogen ◽

Short Term ◽

Graded Response ◽

High K ◽

Human Placental Tissue ◽

High Concentrations ◽

Free Media

SUMMARY Short-term incubation of human placental tissue in Krebs–Ringer bicarbonate buffered media with various concentrations of K+ and Ca2+ showed a graded response in human placental lactogen (HPL) release at different Ca2+ concentrations, but no effect at increased K+ concentration. Media with high Ca2+ caused an inhibition of release, while Ca2+-free media caused a stimulation in HPL release. High concentrations of Mg2+ inhibited release minimally, while Ba2+ had no effect. There was no change in HPL release when Na+ concentration was increased. La3+-Locke's solution markedly inhibited release of HPL but the significance of this effect is unknown. These results suggest that Ca2+ is not required for HPL secretion from placental tissue. It seems that HPL secretion in vitro does not follow the usual pattern where a physiological stimulus or high K+ concentration causes inward movement of calcium which couples stimulation to secretion.

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TRANSFORMATION IN VITRO OF [4-14C]DEHYDROEPIANDROSTERONE TO 7-OXYGENATED DERIVATIVES BY NORMAL EARLY HUMAN PLACENTAL TISSUE

Journal of Endocrinology ◽

10.1677/joe.0.0500301 ◽

1971 ◽

Vol 50 (2) ◽

pp. 301-306 ◽

Cited By ~ 10

Author(s):

Y. W. MIRHOM ◽

F. E. SZONTÁGH

Keyword(s):

Placental Tissue ◽

Human Placental Tissue ◽

Early Human ◽

Human Placentae

SUMMARY Samples from three early human placentae were incubated with [4-14C]-dehydroepiandrosterone (DHA). It was found that [4-14C]DHA was transformed into 7-oxygenated derivatives in yields decreasing in the order: 7-oxo-DHA > 7β-hydroxy-DHA > 7α-hydroxy-DHA. These products were only slowly transformed into other derivatives.

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Adrenomedullin gene expression in human placental tIssue and leukocytes: a potential marker of severe tIssue hypoxia in neonates with birth asphyxia

Acta Endocrinologica ◽

10.1530/eje.0.1470711 ◽

2002 ◽

pp. 711-716 ◽

Cited By ~ 24

Author(s):

R Trollmann ◽

E Schoof ◽

E Beinder ◽

D Wenzel ◽

W Rascher ◽

...

Keyword(s):

Gene Expression ◽

Perinatal Asphyxia ◽

Newborn Infants ◽

Birth Asphyxia ◽

Placental Tissue ◽

Tissue Hypoxia ◽

Mrna Levels ◽

Potential Marker ◽

Human Placental Tissue

OBJECTIVE: The aim of the present study was to investigate the role of adrenomedullin (ADM) as a hypoxia-inducible marker of clinically relevant tIssue hypoxia in acute birth asphyxia of term newborn infants. METHODS: For this purpose, ADM mRNA was determined in human placental tIssue of 20 term pregnancies complicated by birth asphyxia (pH and base deficit values, clinical score). In addition, ADM mRNA was measured in leukocytes of the asphyxiated newborn infants during the first 12 h of life (n=12). Controls were available from ten healthy term pregnancies. In vitro, hypoxia-inducible expression of ADM mRNA was evaluated in human choriocarcinoma cells (BeWo) and human leukocytes exposed to hypoxia (1% O(2)) for 1-24 h. mRNA levels were measured by TaqMan real-time PCR. RESULTS: In vitro, ADM mRNA related to porphobilinogen deaminase (PBGD) mRNA levels significantly increased in response to hypoxia within a period of 4 h in leukocytes and 12 h in BeWo cells. In human placental tIssue, significantly higher levels of ADM/PBGD mRNA were present in asphyxiated newborn infants with severe hypoxic-ischemic encephalopathy (HIE) (n=5) compared with patients with mild or no HIE (n=15). Increased levels of ADM/PBGD mRNA levels were found during the first hours of life in leukocytes of neonates with severe HIE compared with controls. CONCLUSIONS: Our results indicate an upregulation of ADM gene expression in human placenta and leukocytes in clinically relevant hypoxic-ischemic birth complications and suggest ADM gene expression as a promising marker for severe complications due to perinatal asphyxia such as HIE.

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